CAMK2D (NM_172127) Human Untagged Clone

CAT#: SC323389

CAMK2D (untagged)-Kinase deficient mutant (K43M) of Human calcium/calmodulin-dependent protein kinase II delta (CAMK2D), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAMK2D
• Synonyms: CAMKD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_172127, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTTCGACCACAACCTGCACCAGGTTCACGGACGAGTATCAGCTTTTCGAGGAGCTTGGAAAGGGGG
  CATTCTCAGTGGTGAGAAGATGTATGAAAATTCCTACTGGACAAGAATATGCTGCCAAAATTATCAACAC
  CAAAAAGCTTTCTGCTAGGGATCATCAGAAACTAGAAAGAGAAGCTAGAATCTGCCGTCTTTTGAAGCAC
  CCTAATATTGTGCGACTTCATGATAGCATATCAGAAGAGGGCTTTCACTACTTGGTGTTTGATTTAGTTA
  CTGGAGGTGAACTGTTTGAAGACATAGTGGCAAGAGAATACTACAGTGAAGCTGATGCCAGTCATTGTAT
  ACAGCAGATTCTAGAAAGTGTTAATCATTGTCACCTAAATGGCATAGTTCACAGGGACCTGAAGCCTGAG
  AATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCTTAGCCATAGAAG
  TTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGATATCTTTCTCCAGAAGTTTTACG
  TAAAGATCCTTATGGAAAGCCAGTGGATATGTGGGCATGTGGTGTCATTCTCTATATTCTACTTGTGGGG
  TATCCACCCTTCTGGGATGAAGACCAACACAGACTCTATCAGCAGATCAAGGCTGGAGCTTATGATTTTC
  CATCACCAGAATGGGACACGGTGACTCCTGAAGCCAAAGACCTCATCAATAAAATGCTTACTATCAACCC
  TGCCAAACGCATCACAGCCTCAGAGGCACTGAAGCACCCATGGATCTGTCAACGTTCTACTGTTGCTTCC
  ATGATGCACAGACAGGAGACTGTAGACTGCTTGAAGAAATTTAATGCTAGAAGAAAACTAAAGGGTGCCA
  TCTTGACAACTATGCTGGCTACAAGGAATTTCTCAGCAGCCAAGAGTTTGTTGAAGAAACCAGATGGAGT
  AAAGGAGTCAACTGAGAGTTCAAATACAACAATTGAGGATGAAGATGTGAAAGCACGAAAGCAAGAGATT
  ATCAAAGTCACTGAACAACTGATCGAAGCTATCAACAATGGGGACTTTGAAGCCTACACAAAAATCTGTG
  ACCCAGGCCTTACTGCTTTTGAACCTGAAGCTTTGGGTAATTTAGTGGAAGGGATGGATTTTCACCGATT
  CTACTTTGAAAATGCTTTGTCCAAAAGCAATAAACCAATCCACACTATTATTCTAAACCCTCATGTACAT
  CTGGTAGGGGATGATGCCGCCTGCATAGCATATATTAGGCTCACACAGTACATGGATGGCAGTGGAATGC
  CAAAGACAATGCAGTCAGAAGAGACTCGTGTGTGGCACCGCCGGGATGGAAAGTGGCAGAATGTTCATTT
  TCATCGCTCGGGGTCACCAACAGTACCCATCAAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_172127 unedited
  ACGCCCGTATCAGCAAATGGGCGGTAGGCGCTGTACGGCTGTGGAGGTCTATATAAGCAGAGCTCGTTTA
  GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATGCCGCGCGTCT
  GCTCGCTTCTGGTCCGTCGCGCTCCCAGCCAGGGCACAGCCCGGACCGAGGATGGCTTCGACCACAACCT
  GCACCAGGTTCACGGACGAGTATCAGCTTTTCGAGGAGCTTGGAAAGGGGGCATTCTCAGTGGTGAGAAG
  ATGTATGAAAATTCCTACTGGACAAGAATATGCTGCCATGATTATCAACACCAAAAAGCTTTCTTGCGTA
  GGTGCCATCAGAAACTTAGAAAAGAGAAGCTCTGAGATTCTGTGCCCGTCCTTTTGAAACCACCCCTAAA
  TATTGGGGCGAGACTGTCATGGAAAAGCATAATCAAAAAAAGGGCTTTTCACTAACTGGGGGGTTGGATT
  TAATTAACCGGGGAGGTGGACCTGGTTGGAAAAACTAATGGGCAGAGAAAAAAACATCAATGGAAGCCGT
  GAGCCCGTTTCATTGGATCACACCCGAAATTCGAAAAATGTTTAATACTTGGTACCCTCTAATGGCTAAA
  TGTTCACGGGGACCCAGAACCCGTGAAAATTTGCCTTTACTAGACAAAATCCAGGGGAACCCCCTGGAAA
  ATTGGGCAAAATTTTGGCTTTACCCAAAAAATTTTAAGGGGGACCCCCCGCGGTGGGGTTGTTGTTTTTG
  GGGCCCCCCGGGAATTTTTTTCCCCCAAATTTTTCTTCTAGATCTCCATTGAGAACCCCCGGGGATATTG
  GGGGCCTGGGGGCCATCTCTCTCATCTCCCTCTGGGGGGGAGGCCCCCCCCTCTCGGGGGGGGAGAACCC
  CAACTCATCCTACAATCTATGGCGGCGGAGACATATTTTTTTCTCCACCCCCAAGAGGCGAGCGGTGAGT
  ACTCTAGCGCACCAAACACATCCTTCAAAGAGTGGTCTTTATCATCTGCTAGAGACGAGCACACCAGCTA
  GACAGACAGCGAGACCAATGCCGTGTAGTACGCCGTTGTCATTCTACTC
  
  Kinase domain sequence

  >SC323389 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CKCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATGCCGCGCGTCTGCTCGCTTCT
  GGTCCGTCGCGCTCCCAGCCAGGGCACAGCCCGGACCGAGGATGGCTTCGACCACAACCTGCACCAGGTT
  CACGGACGAGTATCAGCTTTTCGAGGAGCTTGGAAAGGGGGCATT
  
• Restriction Sites: Please inquire
• ACCN: NM_172127
• Insert Size: 4100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172127.1, NP_742125.1
• RefSeq Size: 4098 bp
• RefSeq ORF: 1437 bp
• Locus ID: 817
• Cytogenetics: 4q26
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, ErbB signaling pathway, Glioma, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Wnt signaling pathway
• Gene Summary: 'The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns.[provided by RefSeq, Nov 2008]'
  Transcript Variant: This variant (1) has multiple differences in the 3' coding region, compared to variant 3. The resulting isoform (1) has a shorter C-terminus, compared to isoform 3. Isoform 1 is encoded by transcript variants 1 and 4. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The extend of this RefSeq trasncript is supported by transcript alignments.