PRKACG (NM_002732) Human Untagged Clone

CAT#: SC323393

PRKACG (untagged)-Kinase deficient mutant (K73M) of Human protein kinase, cAMP-dependent, catalytic, gamma (PRKACG)


  "NM_002732" in other vectors (7)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PRKACG
Synonyms BDPLT19; KAPG; PKACg
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323393 sequence for NM_002732 edited (data generated by NextGen Sequencing)
ATGGGCAACGCCCCCGCCAAGAAGGACACCGAGCAGGAGGAGAGCGTGAACGAGTTCCTA
GCCAAAGCCAGAGGAGATTTCCTCTACAGATGGGGAAACCCCGCTCAAAACACCGCCAGC
TCGGATCAGTTCGAACGGCTCAGGACGCTGGGCATGGGCTCCTTCGGGCGGGTGATGCTG
GTGAGGCACCAGGAGACCGGCGGCCACTACGCCATGATGATCCTCAACAAGCAGAAGGTG
GTGAAGATGAAGCAGGTCGAGCACATACTGAACGAGAAGCGCATCCTGCAGGCGATCGAC
TTTCCGTTCCTCGTCAAACTCCAGTTCTCCTTTAAGGACAACTCCTACCTGTACCTGGTG
ATGGAGTACGTGCCGGGTGGGGAGATGTTCTCCCGCCTACAGCGCGTCGGAAGGTTTAGC
GAGCCCCATGCCTGTTTCTATGCCGCCCAGGTCGTCCTGGCCGTCCAGTACCTACACTCG
CTCGACCTCATCCACCGCGACCTGAAGCCCGAGAATCTCCTCATCGACCAGCAGGGCTAC
CTGCAGGTGACGGACTTCGGTTTCGCCAAGCGCGTGAAGGGCCGCACTTGGACCTTGTGC
GGGACCCCAGAGTACCTGGCCCCCGAGATCATCCTGAGCAAAGGCTACAACAAGGCCGTG
GACTGGTGGGCCCTAGGGGTGCTCATCTATGAGATGGCCGTGGGCTTCCCACCCTTCTAC
GCCGACCAGCCCATCCAGATCTACGAGAAGATCGTCTCTGGGAGGGTGCGGTTTCCCTCC
AAACTCAGCTCTGACCTCAAGCATCTGCTGCGGAGCCTGCTGCAGGTGGACCTCACCAAG
CGCTTCGGAAACCTCAGGAACGGGGTTGGCGACATCAAGAACCACAAGTGGTTCGCCACA
ACCAGCTGGATCGCCATCTATGAGAAGAAGGTGGAAGCTCCCTTCATCCCGAAGTACACA
GGCCCTGGGGATGCCAGTAACTTTGACGACTACGAGGAGGAAGAGCTCCGGATCTCCATC
AATGAGAAGTGTGCCAAGGAGTTTTCTGAGTTTTAG

Clone variation with respect to NM_002732.3
218 a=>t;318 g=>a
>OriGene 5' read for mutant NM_002732 unedited
CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGATGCGTGAGGTCTATATAAGCAGAGCTCGTTTAG
TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCCCTGCCGCC
GCCACCGCCATGGGCAACGCCCCCGCCAAGAAGGACACCGAGCAGGAGGAGAGCGTGAACGAGTTCCTAG
CCAAAGCCAGAGGAGATTTCCTCTACAGATGGGGAAACCCCGCTCAAAACACCGCCAGCTCGGATCAGTT
CGAACGGCTCAGGACGCTGGGCATGGGCTCCTTCGGGCGGGTGATGCTGGTGAGGCACCAGGAGACCGGC
GGCCACTACGCCATGATGATCCTCAACAAGCAGACGTGGTGAAGATGAAGCAGTCGAGCACATACTGACG
AGAGCGCATCCTGCAGGCGATCGACTTTCCGTTCCTCGTCAACTCCAGTTCTCCTTTAGGACACTCCTAC
CTGTACCTGTGATGAGTACGTGCTGGTGGGAGATGTCTCCGCTACAGCGCGTCGAGGTTACGAGCCATGC
TGTTCATGCCGCCAGTCGTCTGCGTCAGTACTACATCGCTCGACCTATCACCGGGACTGGAGCCGAAATC
TCTATCGACCACAGGCCTACCTGGAGGTGAACGGACTCGGTTTCCAGCCGTGAAGGCCGACTTGGACCTT
GGCGGAACAGATACCTGCCTGATATCTCTGACTAGGTCAACACGGGCTGACGTGACCATAGGGCTCTATT
AGAATGCCTGCTTCACCTACGCAACGCACATCTGAGATGTTCTGAGGACTGCGTTCTCACCTCTGATCAC
TGAGCGCAATCTGAAGAGA
>SC323393 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSACGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCCCTGCCGCCGCCACCGCC
ATGGGCAACGCCCCCGCCAAGAAGGACACCGAGCAGGAGGAGAGCGTGAACGAGTTCCTAGCCAAAGCCA
GAGGAGATTTCCTCTACAGATGGGGAAACCCCGCTCAAAACACCG
Restriction Sites Please inquire     
ACCN NM_002732
Insert Size 2100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002732.2, NP_002723.2
RefSeq Size 1560 bp
RefSeq ORF 1056 bp
Locus ID 5568
Cytogenetics 9q21.11
Domains pkinase, S_TK_X, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway
Gene Summary 'Cyclic AMP-dependent protein kinase (PKA) consists of two catalytic subunits and a regulatory subunit dimer. This gene encodes the gamma form of its catalytic subunit. The gene is intronless and is thought to be a retrotransposon derived from the gene for the alpha form of the PKA catalytic subunit. [provided by RefSeq, Jul 2008]'

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