PRKACG (NM_002732) Human Untagged Clone

CAT#: SC323393

PRKACG (untagged)-Kinase deficient mutant (K73M) of Human protein kinase, cAMP-dependent, catalytic, gamma (PRKACG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKACG
• Synonyms: BDPLT19; KAPG; PKACg
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323393 sequence for NM_002732 edited (data generated by NextGen Sequencing)
  ATGGGCAACGCCCCCGCCAAGAAGGACACCGAGCAGGAGGAGAGCGTGAACGAGTTCCTA
  GCCAAAGCCAGAGGAGATTTCCTCTACAGATGGGGAAACCCCGCTCAAAACACCGCCAGC
  TCGGATCAGTTCGAACGGCTCAGGACGCTGGGCATGGGCTCCTTCGGGCGGGTGATGCTG
  GTGAGGCACCAGGAGACCGGCGGCCACTACGCCATGATGATCCTCAACAAGCAGAAGGTG
  GTGAAGATGAAGCAGGTCGAGCACATACTGAACGAGAAGCGCATCCTGCAGGCGATCGAC
  TTTCCGTTCCTCGTCAAACTCCAGTTCTCCTTTAAGGACAACTCCTACCTGTACCTGGTG
  ATGGAGTACGTGCCGGGTGGGGAGATGTTCTCCCGCCTACAGCGCGTCGGAAGGTTTAGC
  GAGCCCCATGCCTGTTTCTATGCCGCCCAGGTCGTCCTGGCCGTCCAGTACCTACACTCG
  CTCGACCTCATCCACCGCGACCTGAAGCCCGAGAATCTCCTCATCGACCAGCAGGGCTAC
  CTGCAGGTGACGGACTTCGGTTTCGCCAAGCGCGTGAAGGGCCGCACTTGGACCTTGTGC
  GGGACCCCAGAGTACCTGGCCCCCGAGATCATCCTGAGCAAAGGCTACAACAAGGCCGTG
  GACTGGTGGGCCCTAGGGGTGCTCATCTATGAGATGGCCGTGGGCTTCCCACCCTTCTAC
  GCCGACCAGCCCATCCAGATCTACGAGAAGATCGTCTCTGGGAGGGTGCGGTTTCCCTCC
  AAACTCAGCTCTGACCTCAAGCATCTGCTGCGGAGCCTGCTGCAGGTGGACCTCACCAAG
  CGCTTCGGAAACCTCAGGAACGGGGTTGGCGACATCAAGAACCACAAGTGGTTCGCCACA
  ACCAGCTGGATCGCCATCTATGAGAAGAAGGTGGAAGCTCCCTTCATCCCGAAGTACACA
  GGCCCTGGGGATGCCAGTAACTTTGACGACTACGAGGAGGAAGAGCTCCGGATCTCCATC
  AATGAGAAGTGTGCCAAGGAGTTTTCTGAGTTTTAG
  
  Clone variation with respect to NM_002732.3
  218 a=>t;318 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002732 unedited
  CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGATGCGTGAGGTCTATATAAGCAGAGCTCGTTTAG
  TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCCCTGCCGCC
  GCCACCGCCATGGGCAACGCCCCCGCCAAGAAGGACACCGAGCAGGAGGAGAGCGTGAACGAGTTCCTAG
  CCAAAGCCAGAGGAGATTTCCTCTACAGATGGGGAAACCCCGCTCAAAACACCGCCAGCTCGGATCAGTT
  CGAACGGCTCAGGACGCTGGGCATGGGCTCCTTCGGGCGGGTGATGCTGGTGAGGCACCAGGAGACCGGC
  GGCCACTACGCCATGATGATCCTCAACAAGCAGACGTGGTGAAGATGAAGCAGTCGAGCACATACTGACG
  AGAGCGCATCCTGCAGGCGATCGACTTTCCGTTCCTCGTCAACTCCAGTTCTCCTTTAGGACACTCCTAC
  CTGTACCTGTGATGAGTACGTGCTGGTGGGAGATGTCTCCGCTACAGCGCGTCGAGGTTACGAGCCATGC
  TGTTCATGCCGCCAGTCGTCTGCGTCAGTACTACATCGCTCGACCTATCACCGGGACTGGAGCCGAAATC
  TCTATCGACCACAGGCCTACCTGGAGGTGAACGGACTCGGTTTCCAGCCGTGAAGGCCGACTTGGACCTT
  GGCGGAACAGATACCTGCCTGATATCTCTGACTAGGTCAACACGGGCTGACGTGACCATAGGGCTCTATT
  AGAATGCCTGCTTCACCTACGCAACGCACATCTGAGATGTTCTGAGGACTGCGTTCTCACCTCTGATCAC
  TGAGCGCAATCTGAAGAGA
  
  Kinase domain sequence

  >SC323393 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSACGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCCCTGCCGCCGCCACCGCC
  ATGGGCAACGCCCCCGCCAAGAAGGACACCGAGCAGGAGGAGAGCGTGAACGAGTTCCTAGCCAAAGCCA
  GAGGAGATTTCCTCTACAGATGGGGAAACCCCGCTCAAAACACCG
  
• Restriction Sites: Please inquire
• ACCN: NM_002732
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002732.2, NP_002723.2
• RefSeq Size: 1560 bp
• RefSeq ORF: 1056 bp
• Locus ID: 5568
• Cytogenetics: 9q21.11
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway
• Gene Summary: 'Cyclic AMP-dependent protein kinase (PKA) consists of two catalytic subunits and a regulatory subunit dimer. This gene encodes the gamma form of its catalytic subunit. The gene is intronless and is thought to be a retrotransposon derived from the gene for the alpha form of the PKA catalytic subunit. [provided by RefSeq, Jul 2008]'