YANK2 (STK32B) (NM_018401) Human Untagged Clone

CAT#: SC323410

STK32B (untagged)-Kinase deficient mutant (K52M) of Human serine/threonine kinase 32B (STK32B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STK32B
• Synonyms: HSA250839; STK32; STKG6; YANK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323410 sequence for NM_018401 edited (data generated by NextGen Sequencing)
  ATGGGCGGGAACCACTCCCACAAGCCCCCCGTGTTTGACGAGAATGAGGAAGTCAACTTT
  GACCATTTTCAGATTCTGCGGGCCATTGGTAAAGGGAGTTTTGGAAAGGTATGCATCGTG
  CAGAAGCGAGACACTAAGAAAATGTATGCAATGATGTACATGAACAAGCAGAAGTGCATC
  GAGAGGGATGAGGTTCGGAATGTTTTCCGGGAGCTGCAGATCATGCAAGGGCTGGAGCAC
  CCCTTCCTGGTCAATCTGTGGTACTCCTTCCAGGATGAGGAGGACATGTTCATGGTGGTG
  GACCTGCTCCTGGGAGGCGACCTGCGCTACCATCTGCAGCAGAATGTGCATTTCACAGAG
  GGGACTGTGAAACTCTACATCTGTGAGCTGGCACTGGCCCTGGAGTATCTTCAGAGGTAC
  CACATCATCCACAGAGACATCAAGCCAGACAATATCCTGCTGGATGAACACGGACATGTT
  CACATTACAGACTTCAACATAGCGACGGTAGTGAAAGGAGCAGAAAGGGCTTCCTCCATG
  GCTGGCACCAAGCCCTACATGGCTCCAGAAGTATTCCAGGTGTACATGGACAGAGGCCCC
  GGATACTCGTACCCTGTCGACTGGTGGTCCCTGGGCATCACAGCCTATGAGCTGCTGCGG
  GGCTGGAGGCCGTACGAAATCCACTCGGTCACGCCCATCGATGAAATCCTCAACATGTTC
  AAGGTGGAGCGTGTCCACTACTCCTCCACGTGGTGCAAGGGGATGGTGGCCCTGCTGAGG
  AAGCTCCTGACCAAGGATCCTGAGAGCCGCGTGTCCAGCCTTCATGACATACAGAGCGTG
  CCCTACTTGGCCGACATGAACTGGGACGCGGTGTTCAAGAAGGCACTGATGCCCGGCTTT
  GTGCCCAATAAAGGGAGGTTGAACTGCGATCCCACATTTGAGCTTGAAGAGATGATTCTA
  GAATCCAAGCCACTTCACAAAAAGAAGAAGCGATTGGCAAAGAACAGATCCAGGGATGGC
  ACAAAGGACAGCTGCCCGCTGAATGGACACCTGCAGCACTGTTTGGAGACTGTCCGGGAG
  GAATTCATCATATTCAACAGAGAGAAGCTCAGGAGGCAGCAGGGACAGGGCAGCCAGCTC
  TTGGACACCGACAGCCGAGGGGGAGGCCAGGCCCAAAGCAAGCTCCAGGACGGGTGCAAC
  AACAACCTCCTCACCCACACCTGCACCCGTGGCTGCAGCAGCTGA
  
  Clone variation with respect to NM_018401.1
  155 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_018401 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGGGACGCCGTCC
  CCGCCCCTGCACGGTGCTCGGCCCCCTCGGGCTCCGCGCGCGGCTACAACCCGGACTGGGCGCGCCCCCG
  GCATCCCGCATCTCTGCGCGCGTCCCACATCCCGCATCCGGCATCCCAGCGGCCGGGCATGTAGCAGCGG
  CAGCAACGGCGGAATATGGGCGGGAACCACTCCCACAAGCCCCCCCGTGTTTGACGAGAATGAGGAAGTC
  AACTTTGACCATTTTCAGATTCTGCGGGCCATTGGTAAAGGGAGTTTTGGAAAGGTATGCATCGTGCAGA
  AGCGAGACACTAAGAAAATGTATGCAATGATGTACATGACAAGCAGAGTGCATCGAGAGGGATGAGTTCG
  GATGTTTTCCGGAGCTGCAGATCATGCAGGCTGAGCCACCCCTCCTGTCATCCTGTGTACTCTCAGATGA
  GAGCCATTGTCCATGGTGGTGGAACTTGCTCTGGAGGCCGACTGGCGCTAACATCTGCAAGCGAAAGGTG
  CCATGCACAAGGGAACGTGTAACTCTCACTCGTGTAGACTCTGACACTTGGCCCTTGGAAGTTATCTCCT
  CAAGGTTACCATCTCCTCAAGAGCACTCTAAGGCCGAGCACAATCTGTCGTGATGAGACGGCACTGTGTC
  ATTACGACGTCTTCACTATCGACCGTGGTGTAAGAGCCAAAGGGCTCTCTCATAGTGTGAGACCAAGCGC
  TCATGGGTCTCAGATCTACGGGTTCTTGTGACAAGCGGATCTGAACTGCACTGTGTCCTGGTCATCATTA
  CGCTGCAGCGTGAGAGCTACTGATC
  
  Kinase domain sequence

  >SC323410 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CCTTGMGCATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAG
  AATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGGGACGCCGTCCCCGCCCC
  TGCACGGTGCTCGGCCCCCTCGGGCTCCGCGCGCGGCTACAACCCGGACTGGGCGCGCCCCCGGCATCCC
  GCATCTCTGCGCGCGTCCCACATCCCGCATCCGGCATCCCAGCGG
  
• Restriction Sites: Please inquire
• ACCN: NM_018401
• ORF Size: 1245 bp
• Insert Size: 3900
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_018401.1, NP_060871.1
• RefSeq Size: 3224
• RefSeq ORF: 1245
• Locus ID: 55351
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a serine-threonine protein kinase. Serine-threonine kinases transfer phosphate molecules to the oxygen atoms of serine and threonine. A genomic deletion affecting this gene has been associated with Ellis-van Creveld syndrome, an autosomal recessive skeletal dysplasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]