CLK1 (NM_004071) Human Untagged Clone

CAT#: SC323440

CLK1 (untagged)-Kinase deficient mutant (K191M) of Human CDC-like kinase 1 (CLK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLK1
• Synonyms: CLK; CLK/STY; STY
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323440 sequence for NM_004071 edited (data generated by NextGen Sequencing)
  ATGAGACACTCAAAGAGAACTTACTGTCCTGATTGGGATGACAAGGATTGGGATTATGGA
  AAATGGAGGAGCAGCAGCAGTCATAAAAGAAGGAAGAGATCACATAGCAGTGCCCAGGAG
  AACAAGCGCTGCAAATACAATCACTCTAAAATGTGTGATAGCCATTATTTGGAAAGCAGG
  TCTATAAATGAGAAAGATTATCATAGTCGACGCTACATTGATGAGTACAGAAATGACTAC
  ACTCAAGGATGTGAACCTGGACATCGCCAAAGAGACCATGAAAGCCGGTATCAGAACCAT
  AGTAGCAAGTCTTCTGGTAGAAGTGGAAGAAGTAGTTATAAAAGCAAACACAGGATTCAC
  CACAGTACTTCACATCGTCGTTCACATGGGNAGAGTCACCGAAGGAAAAGAACCAGGAGT
  GTAGAGGATGATGAGGAGGGTCACCTGATCTGTCAGAGTGGAGACGTACTAAGTGCAAGA
  NATGAAATTGTTGATACTTTAGGTGAAGGAGCTTTTGGAAAAGTTGTGGAGTGCATCGAT
  CATAAAGCGGGAGGTAGACATGTAGCAGTAATGATAGTTAAAAATGTGGATAGATACTGT
  GAAGCTGCTCGCTCAGAAATACAAGTTCTGGAACATCTGAATACAACAGACCCCAACAGT
  ACTTTCCGCTGTGTCCAGATGTTGGAATGGTTTGAGCATCATGGTCACATTTGCATTGTT
  TTTGAACTATTGGGACTTAGTACTTACGACTTCATTAAAGAAAATGGGTTTCTACCATTT
  CGACTGGATCATATCAGAAAGATGGCATATCAGATATGCAAGTCTGTGAATTTTTTGCAC
  AGTAATAAGTTGACTCACACAGACTTAAAGCCTGAAAACATCTTATTTGTGCAGTCTGAC
  TACACAGAGGCGTATAATCCCAAAATAAAACGTGATGAACGCACCTTAATAAATCCAGAT
  ATTAAAGTTGTAGACTTTGGTAGTGCAACATATGATGACGAACATCACAGTACATTGGTA
  TCTACAAGACATTATAGAGCACCTGAAGTTATTTTAGCCCTAGGGTGGTCCCAACCATGT
  GATGTCTGGAGCATAGGATGCATTCTTATTGAATACTATCTTGGGTTTACCGTATTTCCA
  ACACACGATAGTAAGGAGCATTTAGCAATGATGGAAAGGATTCTTGGACCTCTACCAAAA
  CATATGATACAGAAAACCAGGAAACGTAAATATTTTCACCACGATCGATTAGACTGGGAT
  GAACACAGTTCTGCCGGCAGATATGTTTCAAGACGCTGTAAACCTCTGAAGGAATTTATG
  CTTTCTCAAGATGTTGAACATGAGCGTCTCTTTGACCTCATTCAGAAAATGTTGGAGTAT
  GATCCAGCCAAAAGAATTACTCTCAGAGAAGCCTTAAAGCATCCTTTCTTTGACCTTCTG
  AAGAAAAGTATATAG
  
  Clone variation with respect to NM_004071.3
  391 a=>n;481 t=>n;572 a=>t;573 a=>g;768 t=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004071 unedited
  CGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACC
  GTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGACGCAGTCAGCTG
  CGTGATTCCCGTGATTGCGTTACAAGCTTTGTCTCCTTCGACTTGGAGTCTTTGTCCAGGACGATGAGAC
  ACTCAAAGAGAACTTACTGTCCTGATTGGGATGACAAGGATTGGGATTATGGAAAATGGAGGAGCAGCAG
  CAGTCATAAAAGAAGGAAGAGATCACATAGCAGTGCGCCAGGAGAACAAGCGCTGCAAATACAATCACTC
  TAAAATGTGTGATAGCCATTATTTGGAAAGCAGGTCTATAAATGAGAAAAGATTATCATAGTCGACGCTA
  CATTGATGGAGTACAGAAATGACTCACTCAGGGATGGTGACCCTGGGACATCGCCAAGAAGACAATGAAA
  CCCGTTATCAAACCCATGGTAGCAGTTCTTTCGGGTAAAGTGGGAGAAGGTGGTTATAAAGCCAAAACCA
  GGATTACCCAGGACCTTACCACCGGTGTTTCATTGGGGAAAGACCGTTTTAAAACATTTGGAATTTATTC
  CATGGGAACGGGGAACTTGATTTGTGTGATATATTAAAAGTTTTAAAAGATCTCTCACTTTTACCGGGAG
  ATTTTTGTGTGTTGCCTGGGAGGAATTCCAGAAAAGACACATTGTGTCAATATATATGTGTGGCTCATGC
  ACCCGGAGATGAGGGTATAGTGTATTTGAAGTTGAAATTTCCAAGTCTCACGGTTGTGTGCTCCGAACTG
  CTATGTTCTAGTACCCAGTGACCGACGCTCTGGTACTCTAAGATGTCGTCTGCGCATAGTATATC
  
  Kinase domain sequence

  >SC323440 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TGAMGTMTAGTGCAGATGTWAGAATATTTTTCACACTTATTAACTTTTCAGATAACATAATCTATATATA
  GATTAAGCTTTCAGGGATTTGGAAATCTTTTTTTCTTTCTCTTTTTTGTTTTTGTTTTATTTTTCCATTT
  CTTTTGGTGGGGGGGATTGTATTTTTGCTTTCTTTAGAAATGTAATGTTTGTTATATAGAACTTCCAGAA
  CAGTAATCAAATTAATGAAATTAGACCTAATAATTATGTTTTTTG
  
• Restriction Sites: Please inquire
• ACCN: NM_004071
• ORF Size: 1455 bp
• Insert Size: 2000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_004071.2, NP_004062.2
• RefSeq Size: 1887
• RefSeq ORF: 1455
• Locus ID: 1195
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]
  Transcript Variant: This variant (1) encodes the shorter isoform (1).