MATK (NM_139355) Human Untagged Clone

CAT#: SC323486

MATK (untagged)-Kinase deficient mutant (K263M) of Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MATK
• Synonyms: CHK; CTK; HHYLTK; HYL; HYLTK; Lsk
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323486 sequence for NM_139355 edited (data generated by NextGen Sequencing)
  ATGGCGGGGCGAGGCTCTCTGGTTTCCTGGCGGGCATTTCACGGCTGTGATTCTGCTGAG
  GAACTTCCCCGGGTGAGCCCCCGCTTCCTCCGAGCCTGGCACCCCCCTCCCGTCTCAGCC
  AGGATGCCAACGAGGCGCTGGGCCCCGGGCACCCAGTGTATCACCAAATGCGAGCACACC
  CGCCCCAAGCCAGGGGAGCTGGCCTTCCGCAAGGGCGACGTGGTCACCATCCTGGAGGCC
  TGCGAGAACAAGAGCTGGTACCGCGTCAAGCACCACACCAGTGGACAGGAGGGGCTGCTG
  GCAGCTGGGGCGCTGCGGGAGCGGGAGGCCCTCTCCGCAGACCCCAAGCTCAGCCTCATG
  CCGTGGTTCCACGGGAAGATCTCGGGCCAGGAGGCTGTCCAGCAGCTGCAGCCTCCCGAG
  GATGGGCTGTTCCTGGTGCGGGAGTCCGCGCGCCACCCCGGCGACTACGTCCTGTGCGTG
  AGCTTTGGCCGCGACGTCATCCACTACCGCGTGCTGCACCGCGACGGCCACCTCACAATC
  GATGAGGCCGTGTTCTTCTGCAACCTCATGGACATGGTGGAGCATTACAGCAAGGACAAG
  GGCGCTATCTGCACCAAGCTGGTGAGACCAAAGCGGAAACACGGGACCAAGTCGGCCGAG
  GAGGAGCTGGCCAGGGCGGGCTGGTTACTGAACCTGCAGCATTTGACATTGGGAGCACAG
  ATCGGAGAGGGAGAGTTTGGAGCTGTCCTGCAGGGTGAGTACCTGGGGCAAAAGGTGGCC
  GTGAAGAATATCAAGTGTGATGTGACAGCCCAGGCCTTCCTGGACGAGACGGCCGTCATG
  ACGAAGATGCAACACGAGAACCTGGTGCGTCTCCTGGGCGTGATCCTGCACCAGGGGCTG
  TACATTGTCATGGAGCACGTGAGCAAGGGCAACCTGGTGAACTTTCTGCGGACCCGGGGT
  CGAGCCCTCGTGAACACCGCTCAGCTCCTGCAGTTTTCTCTGCACGTGGCCGAGGGCATG
  GAGTACCTGGAGAGCAAGAAGCTTGTGCACCGCGACCTGGCCGCCCGCAACATCCTGGTC
  TCAGAGGACCTGGTGGCCAAGGTCAGCGACTTTGGCCTGGCCAAAGCCGAGCGGAAGGGG
  CTAGACTCAAGCCGGCTGCCCGTCAAGTGGACGGCGCCCGAGGCTCTCAAACACGGGAAG
  TTCACCAGCAAGTCGGATGTCTGGAGTTTTGGGGTGCTGCTCTGGGAGGTCTTCTCATAT
  GGACGGGCTCCGTACCCTAAAATGTCACTGAAAGAGGTGTCGGAGGCCGTGGAGAAGGGG
  TACCGCATGGAACCCCCCGAGGGCTGTCCAGGCCCCGTGCACGTCCTCATGAGCAGCTGC
  TGGGAGGCAGAGCCCGCCCGCCGGCCACCCTTCCGCAAACTGGCCGAGAAGCTGGCCCGG
  GAGCTACGCAGTGCAGGTGCCCCAGCCTCCGTCTCAGGGCAGGACGCCGACGGCTCCACC
  TCGCCCCGAAGCCAGGAGCCCTGA
  
  Clone variation with respect to NM_139355.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_139355 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCCGGCGGCGGCG
  GCGGCGCGCGGAGGAGACCGCAGTGCGGCCGGCGCTAGGACCCGCGGGGGCCTCCCAGGCCGCGGCGCCT
  CGCGCTTTCCCCCACTCCCCGACCCTTCTTCGCCCCCAAAATGAGGAAACGGAGCAACTCGCTCCAAGTT
  GTGCAGCCGGGACCGCCTCGGGGTGTGCAGCCGGGCTCGCGGAGGCCCTCCCTGGGGGCGGGCGCCGGGG
  CGCGGCTCGGGGGCCGCCCCCCTGAGCCAAAAACCAGGAAAAACCAGCCTCCGGTCCAGTGGGCACCCCA
  CCTCCCCTACCCTCCGGTGCAACCCGCCTGGCCGGTGGCAGGCCTTTCCCACCGTCCCCAACGGTAACCA
  CTTGTCAAGTTGGCCTTTCCCCGCCTCATTTTCCTTCGGGGCCCAATGCCGGGCCCAACCTCTGGGTTTC
  CGGGGGGCATTACCGGTGGGAATTTCGCGGGAAACTTCCCGGGGAGCCCCGCTTTCTCCAAACCGGACCC
  CCCCCCCGTTAAACGATACCACAAAGCCGGGGCCCGGCCCCCCAGTGTACCCATACGCACCCCTCCCAAA
  CGAGAGAGGCGCCTCAAGGGGCAGTGCAACCTTAGAGCGGAGAGACGAGCGAGCGACTGCCCACCCCC
  
  Kinase domain sequence

  >SC323486 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CAMGGGCTGGTMTGACTGCAGCATTTGACATTGGGAGCACAGATCGGAGAGGGAGAGTTTGGAGCTGTCC
  TGCAGGGTGAGTACCTGGGGCAAAAGGTGGCCGTGATGAATATCAAGTGTGATGTGACAGCCCAGGCCTT
  CCTGGACGAGACGGCCGTCATGACGAAGATGCAACACGAGAACCTGGTGCGTCTCCTGGGCGTGATCCTG
  CACCAGGGGCTGTACATTGTCATGGAGCACGTGAGCAAGGGCAAC
  
• Restriction Sites: Please inquire
• ACCN: NM_139355
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139355.1, NP_647612.1
• RefSeq Size: 2183 bp
• RefSeq ORF: 1524 bp
• Locus ID: 4145
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Gene Summary: 'The protein encoded by this gene has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site. This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the predominant isoform a.