MEK5 (MAP2K5) (NM_145161) Human Untagged Clone

CAT#: SC323547

MAP2K5 (untagged)-Kinase deficient mutant (K195M) of Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant C

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP2K5
• Synonyms: dual specificity mitogen-activated protein kinase kinase 5; HsT17454; MAPK/ERK kinase 5; MAP kinase kinase MEK5b; MAPKK5; MEK5; MEK5, MAPKK5, PRKMK5, HsT17454; mitogen-activated protein kinase kinase 5; PRKMK5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_145161, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGTGGCTAGCCCTTGGCCCCTTTCCTGCCATGGAGAACCAGGTGCTGGTAATTCGCATCAAGATCC
  CAAATAGTGGCGCGGTGGACTGGACAGTGCACTCCGGGCCGCAGTTACTCTTCAGGGATGTGCTGGATGT
  GATAGGCCAGGTTCTGCCTGAAGCAACAACTACAGCATTTGAATATGAAGATGAAGATGGTGATCGAATT
  ACAGTGAGAAGTGATGAGGAAATGAAGGCAATGCTGTCATATTATTATTCCACAGTAATGGAACAGCAAG
  TAAATGGACAGTTAATAGAGCCTCTGCAGATATTTCCAAGAGCCTGCAAGCCTCCTGGGGAACGGAACAT
  ACATGGCCTGAAGGTGAATACTCGGGCCGGACCCTCTCAACACAGCAGCCCAGCAGTCTCAGATTCACTT
  CCAAGCAATAGCTTAAAGAAGTCTTCTGCTGAACTGAAAAAAATACTAGCCAATGGCCAGATGAATGAAC
  AAGACATACGATATCGGGACACTCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCATATCATGTCCC
  GAGTGGGAAAATATTAGCTGTAAAGGTCATACTACTAGATATTACACTGGAACTTCAGAAGCAAATTATG
  TCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATTTTATGGAGCATTTTTTGTAG
  AAAACAGGATTTCAATATGTACAGAATTCATGGATGGGGGATCTTTGGATGTATATAGGAAAATGCCAGA
  ACATGTCCTTGGAAGAATTGCAGTAGCAGTTGTTAAAGGCCTTACTTATTTGTGGAGTTTAAAGATTTTA
  CATAGAGACGTGAAGCCCTCCAATATGCTAGTAAACACAAGAGGACAGGTTAAGCTGTGTGATTTTGGAG
  TTAGCACTCAGCTGGTGAATTCTATAGCCAAGACGTATGTTGGAACAAATGCTTATATGGCGCCTGAAAG
  GATTTCAGGGGAGCAGTATGGAATTCATTCTGATGTCTGGAGCTTAGGAATCTCTTTTATGGAGATTCAG
  AAAAACCAGGGATCTTTAATGCCTCTCCAGCTTCTGCAGTGCATTGTTGATGAGGATTCGCCCGTCCTTC
  CAGTTGGAGAGTTCTCGGAGCCATTTGTACATTTCATCACTCAGTGTATGCGAAAACAGCCAAAAGAAAG
  GCCAGCACCTGAAGAATTGATGGGCCACCCGTTCATCGTGCAGTTCAATGATGGAAATGCCGCCGTGGTG
  TCCATGTGGGTGTGCCGGGCGCTGGAGGAGAGGCGGAGCCTGGCTTCCCTGCCCTCGCCTTCACCTCTGT
  CAGCAGGTGGCCTTGCCTGGGGAGCCCCATGTGTGGCCCACCCCACCAGGCCATCCCCATACCTTCTGGT
  TTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_145161 unedited
  ACGCCCGTCTCAGCAATGGGCGGTAGGCGCTGTACGGTGGGAGGATCTATATAAGCAGAGCTCGTTTAGT
  GAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGACCTTCAC
  CATAGCGTTCGCTCAACTCCAGAACCTTCCGACCTCCGCTAGTTCCTGCGGGCCTTTGCCCGCTTCCCGG
  TGCACCCTCCCCGGGAGACACCTCAGACCCCCGACAGCCTGGGCAGGCTCGGTGCCTGCGGGTGCGTTCC
  TGATCACCCCTCCCCTCTTCCCTCCCCCCTCATCCCTCCATTCCCCTTGTTTTTCACCCCTCTGTCCTCT
  GCCCGTCACTCCCCCTTGGTCACCTCTTTGGAGCCCCCCTTCCTACCCAGCGGCAAGTGGGGTTTTCCCA
  TACCCCCAGAAGGTGGAGCCTTCTTTTAACCTGGTATTGCTGGTGGCTTAGCCCTGGGCCCCTTTCTGGC
  ATTGGGAAAAACAAGTTGCTGTTATTTGGCATCAGGATCCCAATAGTGGGCCGTTGAACTGACCGTTGCA
  TTCCGGCCGCGAGTATTCTCTTCAGGGAGTGCTGGATGTGTGATAGCCCAGTTTTTGCCGAAACACACCT
  CAACCGCATTTAAATATAGATAAGATAGTGATATCATTTACTGTGAATGTGAGAGATGAGGGCAGTGTGT
  CATATATTCTCCACTATGTGCACACGTTAGTGACTTATATAACTCTCTGATATTCCAGACGCGACCCTCG
  GAAGACAACATAGCGCAGGAGTATCGCGCGATCTTAACAGCACAGCTGGTATCTACGACTATAAACTCTC
  TGTACGAAAATCACTCGCGCGTATGTACCACCATTGAACCTGATGACGCAGACCTACAACATGCTGGGAT
  TACTGGTGACACACAT
  
  Kinase domain sequence

  >SC323547 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GCAGATGATGACAGACATACGATATCGGGACACTCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCA
  TATCATGTCCCGAGTGGGAAAATATTAGCTGTAATGGTCATACTACTAGATATTACACTGGAACTTCAGA
  AGCAAATTATGTCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATTTTATGGAGC
  ATTTTTTGTAGAAAACAGGATTTCAATATGTACAGAATTCGGCAC
  
• Restriction Sites: Please inquire
• ACCN: NM_145161
• Insert Size: 5500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145161.1, NP_660144.1
• RefSeq Size: 2229 bp
• RefSeq ORF: 2229 bp
• Locus ID: 5607
• Cytogenetics: 15q23
• Domains: PB1, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, MAPK signaling pathway, Neurotrophin signaling pathway
• Gene Summary: 'The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011]'
  Transcript Variant: This variant (C) encodes the longest isform (C).