MEKK3 (MAP3K3) (NM_002401) Human Untagged Clone

CAT#: SC323554

MAP3K3 (untagged)-Kinase deficient mutant (K422M) of Human mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP3K3
• Synonyms: MAPKKK3; MEKK3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323554 sequence for NM_002401 edited (data generated by NextGen Sequencing)
  ATGGACGAACAGGAGGCATTGAACTCAATCATGAACGATCTGGTGGCCCTCCAGATGAAC
  CGACGTCACCGGATGCCTGGATATGAGACCATGAAGAACAAAGACACAGGTCACTCAAAT
  AGGCAGAGTGACGTCAGAATCAAGTTCGAGCACAACGGGGAGAGGCGAATTATAGCGTTC
  AGCCGGCCTGTGAAATATGAAGATGTGGAGCACAAGGTGACAACAGTATTTGGACAACCT
  CTTGATCTACATTACATGAACAATGAGCTCTCCATCCTGCTGAAAAACCAAGATGATCTT
  GATAAAGCAATTGACATTTTAGATAGAAGCTCAAGCATGAAAAGCCTTAGGATATTGCTG
  TTGTCCCAGGACAGAAACCATAACAGTTCCTCTCCCCACTCTGGGGTGTCCAGACAGGTG
  CGGATCAAGGCTTCCCAGTCCGCAGGGGATATAAATACTATCTACCAGCCCCCCGAGCCC
  AGAAGCAGGCACCTCTCTGTCAGCTCCCAGAACCCTGGCCGAAGCTCACCTCCCCCTGGC
  TATGTTCCTGAGCGGCAGCAGCACATTGCCCGGCAGGGGTCCTACACCAGCATCAACAGT
  GAGGGGGAGTTCATCCCAGAGACCAGCGAGCAGTGCATGCTGGATCCCCTGAGCAGTGCA
  GAAAATTCCTTGTCTGGAAGCTGCCAATCCTTGGACAGGTCAGCAGACAGCCCATCCTTC
  CGGAAATCACGAATGTCCCGTGCCCAGAGCTTCCCTGACAACAGACAGGAATACTCAGAT
  CGGGAAACTCAGCTTTATGACAAAGGGGTCAAAGGTGGAACCTACCCCCGGCGCTACCAC
  GTGTCTGTGCACCACAAGGACTACAGTGATGGCAGAAGAACATTTCCCCGAATACGGCGT
  CATCAAGGCAACTTGTTCACCCTGGTGCCCTCCAGCCGCTCCCTGAGCACAAATGGCGAG
  AACATGGGTCTGGCTGTGCAATACCTGGACCCCCGTGGGCGCCTGCGGAGTGCGGACAGC
  GAGAATGCCCTCTCTGTGCAGGAGAGGAATGTGCCAACCAAGTCTCCCAGTGCCCCCATC
  AACTGGCGCCGGGGAAAGCTCCTGGGCCAGGGTGCCTTCGGCAGGGTCTATTTGTGCTAT
  GACGTGGACACGGGACGTGAACTTGCTTCCATGCAGGTCCAATTTGATCCAGACAGTCCT
  GAGACAAGCAAGGAGGTGAGTGCTCTGGAGTGCGAGATCCAGTTGCTAAAGAACTTGCAG
  CATGAGCGCATCGTGCAGTACTATGGCTGTCTGCGGGACCGCGCTGAGAAGACCCTGACC
  ATCTTCATGGAGTACATGCCAGGGGGCTCGGTGAAAGACCAGTTGAAGGCTTACGGTGCT
  CTGACAGAGAGCGTGACCCGAAAGTACACGCGGCAGATCCTGGAGGGCATGTCCTACCTG
  CACAGCAACATGATTGTTCACCGGGACATTAAGGGAGCCAACATCCTCCGAGACTCTGCT
  GGGAATGTAAAGCTGGGGGACTTTGGGGCCAGCAAACGCCTGCAGACGATCTGTATGTCG
  GGGACGGGCATGCGCTCCGTCACTGGCACACCCTACTGGATGAGCCCTGAGGTGATCAGC
  GGCGAGGGCTATGGAAGGAAAGCAGACGTGTGGAGCCTGGGCTGCACTGTGGTGGAGATG
  CTGACAGAGAAACCACCGTGGGCAGAGTATGAAGCTATGGCCGCCATCTTCAAGATTGCC
  ACCCAGCCCACCAATCCTCAGCTGCCCTCCCACATCTCTGAACATGTCCGGGACTTCCTG
  AGGCGCATTTTTGTGGAGGCTCGCCAGAGACCTTCAGCTGAGGAGCTGCTCACACACCAC
  TTTGCACAGCTCATGTACTGA
  
  Clone variation with respect to NM_002401.3
  1172 a=>t;1787 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002401 unedited
  CGTCCGCCATAACAACGGGCGGAGGCGAGAACGGTGGAGGTAAATAAGCAGAGCACGAAAAGCGAACCGT
  CAGAACAAAGAAATACGACACACAAAAGGGCGGCCGCGAATACGGCACCAGGCCCCCCGCGCGGAGCCAG
  GCCCGCTGCCGTCCCCGCCGCCCGGGCCCCCGGCATGCAGCCCCGGCTGGGAGGTGACACTCACGGACCT
  TAGCCACCGCCGCCGCCATCGCCACCATGGACGAACAGGAGGCATTGAACTCAATCATGAACGATCTGGT
  GGCCCTCCAGATGAACCGACGTCACCGGATGCCTGGATATGAGACCATGAAGAACAAAGACACGGTCACT
  CAAATAGGCAGAGTGACGTCAGAATCAAGTTCGAGCACAACGGGGAGAGCGAATATAGCGTTAGCCGGCC
  TGTAAAAATGAAATGTGAGCACAAGGTACAAATTATTGGACAACTTCGAATCTCAAAACATAACAGGAGT
  CTCAACCTCTAAAAAAAAAAATCTGGATAGCAATAATTTTAATAAACCTCAGCTTGAAAACCCTTAGAAT
  TTGGTTGTTTCCCAGGAAAAAACCCTAAAATTTCCTCCCCCCCCTTGGGGGGTTCCAAAACGGGGGCGAT
  CAGGTTTCCCTTCCCCCGGGAATAAAATTATTTTTCCCCCCCCCCCCCCCAAAAAAAGGGCCTTTTTTTT
  TATCCCAAAACCCCGGCCAGAAGACACCCCCCCGTGTATTTTTTTTTAGCGACACCCTTTTGCGCGAGGT
  GCCCTCCAACTCTCTCTGGGGGGGAATTCCCAACAACCAGCC
  
  Kinase domain sequence

  >SC323554 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CTCAGTGCCCCATCACTGGCGCCGGGGAAGCTCCTGGGCCAGGGTGCCTTCGGCAGGGTCTATTTGTGCT
  ATGACGTGGACACGGGACGTGAACTTGCTTCCATGCAGGTCCAATTTGATCCAGACAGTCCTGAGACAAG
  CAAGGAGGTGAGTGCTCTGGAGTGCGAGATCCAGTTGCTAAAGAACTTGCAGCATGAGCGCATCGTGCAG
  TACTATGGCTGTCTGCGGGACCGCGCTGAGAAGACCCTGACCATC
  
• Restriction Sites: Please inquire
• ACCN: NM_002401
• ORF Size: 1881 bp
• Insert Size: 3460
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_002401.3, NP_002392.2
• RefSeq Size: 4750
• RefSeq ORF: 1881
• Locus ID: 4215
• Domains: PB1, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway
• Gene Summary: This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. The resulting isoform (2) lacks an internal region, as compared to isoform 1.