DYRK4 (NM_003845) Human Untagged Clone

CAT#: SC323557

DYRK4 (untagged)-Kinase deficient mutant (K133M) of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DYRK4
• Synonyms: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003845, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGGCCTCAGAGCTCAAGGCTTCAGAAATACCTTTCCACCCTAGCATTAAAACCCAGGATCCCAAGG
  CAGAGGAGAAGTCACCAAAGAAGCAAAAGGTGACTCTGACAGCGGCAGAGGCCCTAAAGCTTTTTAAGAA
  CCAGCTGTCTCCATATGAACAAAGTGAAATCCTGGGCTACGCGGAGCTGTGGTTCCTGGGTCTTGAAGCC
  AAGAAGCTCGACACGGCTCCTGAGAAATTTAGCAAGACGAGTTTTGATGATGAGCATGGCTTCTATCTGA
  AGGTCCTGCATGATCACATTGCCTACCGCTATGAAGTTCTGGAGACAATCGGGAAGGGGTCCTTTGGACA
  GGTGGCCAAGTGCTTGGATCACAAAAACAATGAGCTGGTGGCCCTGAAAATCATCAGGAACAAGAAGAGG
  TTTCACCAGCAGGCCCTGATGGAGCTGAAGATCCTGGAAGCTCTCAGAAAGAAGGACAAAGACAACACCT
  ACAATGTGGTGCATATGAAGGACTTTTTCTACTTTCGCAATCACTTCTGCATCACCTTTGAGCTCCTGGG
  AATCAACTTGTATGAGTTGATGAAGAATAACAACTTTCAAGGCTTCAGTCTGTCCATAGTTCGGCGCTTC
  ACTCTCTCTGTTTTGAAGTGCTTGCAGATGCTTTCGGTAGAGAAAATCATTCACTGTGATCTCAAGCCCG
  AAAATATAGTGCTATACCAAAAGGGCCAAGCCTCTGTTAAAGTCATTGACTTTGGATCAAGCTGTTATGA
  ACACCAGAAAGTATACACGTACATCCAAAGCCGGTTCTACCGATCCCCAGAAGTGATCCTGGGCCACCCC
  TACGACGTGGCCATTGACATGTGGAGCCTGGGCTGCATCACGGCGGAGTTGTACACGGGCTACCCCCTGT
  TCCCCGGGGAGAATGAGGTGGAGCAGCTGGCCTGCATCATGGAGGTGCTGGGTCTGCCGCCAGCCGGCTT
  CATTCAGACAGCCTCCAGGAGACAGACATTCTTTGATTCCAAAGGTTTTCCTAAAAATATAACCAACAAC
  AGGGGGAAAAAAAGATACCCAGATTCCAAGGACCTCACGATGGTGCTGAAAACCTATGACACCAGCTTCC
  TGGACTTTCTCAGAAGGTGTTTGGTATGGGAACCTTCTCTTCGCATGACCCCGGACCAGGCCCTCAAGCA
  TGCTTGGATTCATCAGTCTCGGAACCTCAAGCCACAGCCCAGGCCCCAGACCCTGAGGAAATCCAATTCC
  TTTTTCCCCTCTGAGACAAGGAAGGACAAGGTTCAAGGCTGTCATCACTCGAGCAGAAAAGCAGATGAGA
  TCACCAAAGAGACTACAGAGAAAACAAAAGATAGCCCCACGAAGCATGTTCAGCATTCAGGTGATCAGCA
  GGACTGTCTCCAGCACGGAGCTGACACTGTTCAGCTGCCTCAACTGGTAGACGCTCCCAAGAAGTCAGAG
  GCAGCTGTCGGGGCGGAGGTGTCCATGACCTCCCCAGGACAGAGCAAAAACTTCTCCCTCAAGAACACAA
  ACGTTTTACCCCCTATTGTATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003845 unedited
  CCGCCGTTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCATAACTTCGTATAGCATACATTATACGAA
  GCTTATGGATTCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAGCGTGAGCTGTTGAAAGCCT
  GCAGCTAAACACCAGTGTTACTTCACTCCCCTTTGTGGACACCAAGGGGAAGAAGAATACGGTAAGCTTC
  CCACACATTAGCAAGAAAGTCCTGCTGAAGTCATCCCCTGCTGTATCAGGAGAATCAAGCTCACAATCAG
  ATGCCCGGCCTCAGAGCTCAAGGCTTCAGAAAATACCTTTCCACCCTAGCATTAAAACCCCAGGATCCCC
  AGGCAGAAGGAAAAGTCACCAAAGAGCCAAAGTTGACTCTGACACCGGCGAAGGCCCTAAAGCTTTTAAG
  AACCAGCTGTTTCCTTATGACAAAGTTGAATCCTGGGGCTACCCGGAGCTGTGGGTCCTGGGGCCTGGAC
  CAAGAAGTCGAACCGGTCTCGGAAAATTTACCAGAGCAGTTTGAGAGGAGACCTTGTTTCTACTGAAAGG
  TTCCATGGATCAATGCCCTACGATTGAAGTCCTAGACACTCCGGAAGGGTCCTTGACCGTGTCGCAAGCT
  CTGTTCACACACTATGCCGTGTGCCTTGATATACGAGACAAGAGTTACCCGCGCGTGAGCTAGATATCGG
  ACCCCAGAGACACAACCCATGGTGCTATAGAGATTCTCTCCCCTTGCCCCTGTCCGGACCTGTATAGAGA
  AACCTAGCGCTCGCATTTGCACTCTCGGTGATGCCGCACGGGGAAACCGGGGGG
  
  Kinase domain sequence

  >SC323557 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TMKATCATTGCCTACCGCTATGAGTTCTGGAGACAATCGGGAAGGGGTCCTTTGGACAGGTGGCCAAGTG
  CTTGGATCACAAAAACAATGAGCTGGTGGCCCTGATGATCATCAGGAACAAGAAGAGGTTTCACCAGCAG
  GCCCTGATGGAGCTGAAGATCCTGGAAGCTCTCAGAAAGAAGGACAAAGACAACACCTACAATGTGGTGC
  ATATGAAGGACTTTTTCTACTTTCGCAATCACTTCTGCATCACCT
  
• Restriction Sites: Please inquire
• ACCN: NM_003845
• ORF Size: 1563 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_003845.1, NP_003836.1
• RefSeq Size: 1860
• RefSeq ORF: 1563
• Locus ID: 8798
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes an enzyme that belongs to a conserved family of serine/threonine protein kinases. Members of this dual specificity kinase family are thought to function in the regulation of cell differentiation and proliferation, survival, and in development. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).