MAK (NM_005906) Human Untagged Clone

CAT#: SC323564

MAK (untagged)-Kinase deficient mutant (K33M) of Human male germ cell-associated kinase (MAK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAK
• Synonyms: RP62
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323564 sequence for NM_005906 edited (data generated by NextGen Sequencing)
  ATGAACCGATACACAACCATGAGACAGTTGGGGGACGGCACGTATGGGAGTGTGCTTATG
  GGCAAGAGTAATGAATCCGGGGAGCTGGTGGCCATCAAAAGGATGAAGAGAAAGTTCTAT
  TCTTGGGATGAATGCATGAACTTGAGAGAAGTTAAGTCTCTGAAGAAACTTAATCATGCC
  AATGTTATTAAATTGAAAGAAGTTATCAGAGAAAATGACCATCTTTATTTTATATTTGAA
  TATATGAAAGAAAACCTCTATCAATTAATGAAAGACAGAAACAAGTTGTTCCCTGAATCA
  GTCATCAGAAATATTATGTATCAAATATTGCAAGGGCTGGCTTTTATCCATAAACATGGC
  TTTTTTCATAGGGACATGAAACCAGAAAACTTGCTTTGTATGGGTCCAGAGCTTGTGAAA
  ATTGCTGATTTTGGACTTGCAAGAGAATTAAGGTCACAGCCACCATACACTGATTATGTA
  TCTACCAGATGGTATCGTGCCCCTGAAGTTTTACTGAGATCTTCAGTTTATAGTTCTCCC
  ATTGATGTGTGGGCTGTTGGAAGTATCATGGCTGAACTCTATATGTTAAGGCCACTTTTC
  CCAGGGACAAGTGAGGTCGATGAAATCTTTAAAATTTGCCAAGTTTTAGGGACTCCCAAA
  AAAAGTGACTGGCCAGAAGGATACCAGCTGGCATCCTCTATGAACTTCCGTTTTCCCCAG
  TGTGTTCCTATAAACTTAAAAACTCTTATTCCCAATGCCAGTAATGAAGCTATTCAGCTC
  ATGACCGAAATGTTGAATTGGGATCCAAAGAAACGACCGACAGCAAGCCAGGCATTGAAA
  CACCCATATTTTCAAGTTGGTCAGGTATTAGGCCCTTCGTCAAATCATCTGGAATCAAAA
  CAGTCTTTAAATAAGCAGCTGCAACCATTAGAATCAAAGCCATCTTTAGTTGAGGTAGAG
  CCTAAGCCTCTGCCGGATATAATCGATCAGGTTGTTGGACAACCCCAGCCAAAAACTAGC
  CAGCAGCCACTGCAGCCCATTCAGCCGCCACAGAACCTGAGCGTCCAGCAACCTCCAAAG
  CAACAGAGTCAGGAGAAACCGCCACAAACGCTATTCCCGAGCATCGTCAAAAACATGCCA
  ACTAAGCCAAATGGCACACTGAGTCATAAAAGTGGTAGGAGGCGTTGGGGTCAGACTATC
  TTCAAGTCTGGAGATAGCTGGGAAGAGTTGGAGGACTATGATTTCGGAGCCTCCCATTCC
  AAGAAGCCAAGCATGGGTGTTTTTAAAGAAAAAAGGAAAAAAGATTCTCCATTTCGGCTT
  CCAGAGCCAGTACCCTCAGGCTCCAACCACTCGACAGGGGAAAACAAGAGCTTACCTGCT
  GTTACTTCCCTAAAATCTGATTCCGAATTGTCAACTGCTCCAACCTCTAAACAGTACTAC
  TTGAAACAATCAAGATATCTTCCAGGTGTGAATCCCAAGAAGGTGTCCTTGATAGCCAGT
  GGAAAGGAAATAAACCCCCACACTTGGAGCAACCAGTTATTCCCCAAGTCACTGGGACCC
  GTTGGGGCAGAACTTGCTTTCAAAAGGAGCAATGCAGGAAATCTTGGAAGTTATGCTACT
  TACAATCAGTCAGGATATATTCCTTCCTTTCTCAAAAAAGAAGTGCAGTCAGCTGGCCAG
  AGGATCCACTTAGCACCTCTCAATGCAACGGCTTCAGAATATACCTGGAACACAAAAACT
  GGTCGGGGGCAGTTTTCAGGACGTACTTATAATCCTACAGCAAAAAACCTAAATATTGTG
  AACCGTGCACAGCCCATTCCCTCAGTGCACGGGAGGACAGACTGGGTGGCCAAGTATGGA
  GGCCACCGGTAG
  
  Clone variation with respect to NM_005906.4
  1830 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_005906 unedited
  ACCGCTCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTAATCCCCAGCGG
  GCTGCAGGGCCACCACCCTCTGTCCGACCCAGCCGGAGCCCGACCTGCGAGGCTGGCCGGGCATGTCCCC
  TCGGCGCCCTGTGCTACCAGGGCCAATCTCCGCCGGGAGACCGGTCCCTGACGCCGGGTGTTTATAAGAA
  TGGAAGTGTTGTTTCCTTGCCCGATTCCTTCATGCTATATCTCATGAACCTCTGTAATCTTGGGGGAGAG
  ACTATATTTAATGATGACAAACCTGTCACCAGTGTAGCACCAACAGTGGGGAGGCCAAAACCAAAAAAAA
  TTAAGAACCGTTCAATTTATATTCAACAAGGAAGTCTTTTCATCACCAACTTCGGCTGCATTATTTTTCC
  GAATAACCCGACCCCAACCAGGAAACAGTTGGGGGGACGGCCCGTTGGGAAGTGCCTTAGGGCCAAGAGT
  AAGGATACCGGGGACGGGGGGCACCGGGGGTAGAAAAAAATTTCATTTCTTGGGATGAGTGTGAGACCTG
  AGAAATTTATCCTCAAAAAACTACTGCCCAGTGTTATATTTTGAAATTTTCAAAAAGAGCACTCTTTTTA
  TTTTAATTTTGAAAAAACCCTTCATTTTTAAGAGAAAACCTTTGCCCGACATCTCCAAATATTTTAAATG
  CGGGCGCGGCTATCACAAGCGTTTTAGAGAGACCCAATCTGTTGGTGACAAGTTATACTCGTGTCGTGCG
  AAA
  
  Kinase domain sequence

  >SC323564 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CCATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTAATCCCCAGCGGGCTGCA
  GGGCCACCACCCTCTGTCCGACCCAGCCGGAGCCCGACCTGCGAGGCTGGCCGGGCATGTCCCCTCGGCG
  CCCTGTGCTACCAGGGCCAATCTCCGCCGGGAGACCGGTCCCTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_005906
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005906.3, NP_005897.1
• RefSeq Size: 3830 bp
• RefSeq ORF: 1872 bp
• Locus ID: 4117
• Cytogenetics: 6p24.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) encodes isoform 1.