PRKX (NM_005044) Human Untagged Clone

CAT#: SC323612

PRKX (untagged)-Kinase deficient mutant (K78M) of Human protein kinase, X-linked (PRKX)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKX
• Synonyms: PKX1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323612 sequence for NM_005044 edited (data generated by NextGen Sequencing)
  ATGGAGGCGCCCGGGCTGGCCCAGGCGGCCGCGGCGGAGAGCGACTCCCGCAAGGTGGCG
  GAGGAGACCCCCGACGGGGCGCCCGCGCTCTGCCCCAGCCCTGAGGCGCTGTCGCCGGAG
  CCGCCTGTGTACAGCCTGCAGGACTTTGACACGCTGGCCACCGTGGGCACTGGGACTTTC
  GGGCGGGTGCACCTGGTGAAGGAGAAGACAGCCAAGCATTTCTTCGCCCTCATGGTGATG
  AGCATTCCCGACGTCATCCGCCTAAAGCAGGAGCAACACGTACACAATGAGAAGTCTGTC
  CTGAAGGAAGTCAGCCACCCGTTCCTCATCAGGCTGTTCTGGACGTGGCATGACGAGCGC
  TTCCTCTACATGCTCATGGAGTACGTGCCGGGCGGCGAGCTCTTCAGCTACCTGCGCAAC
  CGGGGGCGCTTCTCCAGCACCACGGGGCTCTTCTACTCTGCAGAGATCATCTGTGCCATC
  GAGTACCTGCACTCCAAAGAGATCGTCTACAGGGACTTGAAGCCAGAGAACATCCTGCTG
  GATAGGGATGGCCACATTAAGCTCACGGACTTTGGGTTCGCCAAGAAGCTGGTAGACAGG
  ACTTGGACCCTCTGTGGAACACCCGAGTACCTAGCCCCCGAAGTCATTCAGAGCAAGGGC
  CACGGAAGGGCCGTGGACTGGTGGGCCCTCGGCATCCTGATATTCGAGATGCTTTCGGGG
  TTTCCTCCGTTTTTTGATGACAACCCGTTTGGCATTTATCAGAAAATTCTTGCAGGCAAA
  ATAGATTTCCCCAGACATTTGGATTTCCATGTAAAAGACCTCATTAAGAAACTGCTCGTG
  GTTGACAGAACAAGGCGATTAGGAAACATGAAGAACGGGGCGAATGATGTGAAGCATCAT
  CGGTGGTTCCGCTCCGTGGACTGGGAAGCTGTTCCGCAGAGAAAACTGAAGCCTCCCATC
  GTGCCCAAGATAGCTGGTGACGGCGACACTTCCAACTTCGAAACTTACCCTGAGAATGAC
  TGGGACACAGCCGCGCCCGTGCCGCAGAAGGATTTAGAAATCTTCAAGAATTTCTGA
  
  Clone variation with respect to NM_005044.4
  177 g=>t;233 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_005044 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCCAACAGCGCGCA
  CGCGGCTACCGAGCTGGAGGAGGCGGCGGGCGCGAGACCCGGAATGCGCAGGGCCCCCGCCTCGCCCCCC
  CCAGCCCGGGCCGCGGCCCCCGCCTTCCCCGCAGTCGTCCCGCACTCGGTGCCCGCCCCCCGAGGCCGGC
  GGCTGCTCCCACTCGGGGCCGTTGCTGCTTTGTGCCGTGAGCGCCGCCCAGCCATTGTCCCCGTCGCTTC
  CGTCAGCCCGCGCCGGAACCGCGCACCAGGAAGGCCAAGAACCGCCCCATGGGGGAGGCCCTCGGTGGAT
  GCGGCGGCGGGGCGGCCTTCGAGAGGCTGCTTCCGGGGAGGCCGGGTTCCCCAAGCCCTCGGGCTTGGCC
  GGGGGCCCGGGACTGGGATGGCGTCCCCAGGGGGGGCCCCGGGCTGGCCCAGGGGGGCCGGGGGGGGAGA
  CCAACCCCCGCAGGGGGGGGGAAGGAAACCCCAAAGGGGGCCCCCGCCCTTGTGCCCAACCCGAGGGGCC
  TTTCCCGGGACCCCCCTGGGCCCCCCGGAAAATTTGACCCCGGGGCCCGTGGGGCATTGGACTTTTGGGG
  GGGGTCCCGCGTAGGAAAAAACCCCCGTTTTTCCCCCCTTAGGGAGAAAAAAGAAG
  
  Kinase domain sequence

  >SC323612 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TMKKGCMCGTGGCMTGGGACTTTCGGGCGGGTGCACCTGGTGAAGGAGAAGACAGCCAAGCATTTCTTCG
  CCCTCATGGTGATGAGCATTCCCGACGTCATCCGCCTAAAGCAGGAGCAACACGTACACAATGAGAAGTC
  TGTCCTGAAGGAAGTCAGCCACCCGTTCCTCATCAGGCTGTTCTGGACGTGGCATGACGAGCGCTTCCTC
  TACATGCTCATGGAGTACGTGCCGGGCGGCGAGCTCTTCAGCTAC
  
• Restriction Sites: Please inquire
• ACCN: NM_005044
• ORF Size: 1077 bp
• Insert Size: 2710
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_005044.1, NP_005035.1
• RefSeq Size: 6034
• RefSeq ORF: 1077
• Locus ID: 5613
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway
• Gene Summary: This gene encodes a serine threonine protein kinase that has similarity to the catalytic subunit of cyclic AMP dependent protein kinases. The encoded protein is developmentally regulated and may be involved in renal epithelial morphogenesis. This protein may also be involved in macrophage and granulocyte maturation. Abnormal recombination between this gene and a related pseudogene on chromosome Y is a frequent cause of sex reversal disorder in XX males and XY females. Pseudogenes of this gene are found on chromosomes X, 15 and Y. [provided by RefSeq, Feb 2010]