CDKL2 (NM_003948) Human Untagged Clone

CAT#: SC323614

CDKL2 (untagged)-Kinase deficient mutant (K33M) of Human cyclin-dependent kinase-like 2 (CDC2-related kinase) (CDKL2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDKL2
• Synonyms: KKIAMRE; P56
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323614 sequence for NM_003948 edited (data generated by NextGen Sequencing)
  ATGGAAAAATATGAAAACCTGGGTTTGGTTGGAGAAGGGAGTTATGGAATGGTGATGAAG
  TGTAGGAATAAAGATACTGGAAGAATTGTGGCCATAATGAAGTTCTTAGAAAGTGACGAT
  GACAAAATGGTTAAAAAGATTGCAATGCGAGAAATCAAGTTACTAAAGCAACTTAGGCAT
  GAAAACTTGGTGAATCTCTTGGAAGTGTGTAAGAAAAAAAAACGATGGTACCTAGTCTTT
  GAATTTGTTGACCACACAATTCTTGATGACTTGGAGCTCTTTCCAAATGGACTAGACTAC
  CAAGTAGTTCAAAAGTATTTGTTTCAGATTATTAATGGAATTGGATTTTGTCACAGTCAC
  AATATCATACACAGAGATATAAAGCCAGAGAATATATTAGTCTCCCAGTCTGGCGTTGTC
  AAGCTATGCGATTTTGGATTTGCGCGAACATTGGCAGCTCCTGGGGAGGTTTATACTGAT
  TATGTGGCAACCCGATGGTACAGAGCTCCAGAACTATTGGTTGGTGATGTCAAGTATGGC
  AAGGCTGTTGATGTGTGGGCCATTGGTTGTCTGGTAACTGAAATGTTCATGGGGGAACCC
  CTATTTCCTGGAGATTCTGATATTGATCAGCTATATCATATTATGATGTGTTTAGGTAAT
  CTAATTCCAAGGCATCAGGAGCTTTTTAATAAAAATCCTGTGTTTGCTGGAGTAAGGTTG
  CCTGAAATCAAGGAAAGAGAACCTCTTGAAAGACGCTATCCTAAGCTCTCTGAAGTGGTG
  ATAGATTTAGCAAAGAAATGCTTACATATTGACCCCGACAAAAGACCCTTCTGTGCTGAG
  CTCCTACACCATGATTTCTTTCAAATGGATGGATTTGCTGAGAGGTTTTCCCAAGAACTA
  CAGTTAAAAGTACAGAAAGATGCCAGAAATGTTTCTTTATCTAAAAAATCCCAAAACAGA
  AAGAAGGAAAAAGAAAAAGATGATTCCTTAGTTGAAGAAAGAAAAACACTTGTGGTACAG
  GATACCAATGCTGATCCCAAAATTAAGGATTATAAACTATTTAAAATAAAAGGCTCAAAA
  ATTGATGGAGAAAAAGCTGAAAAAGGCAATAGAGCTTCAAATGCCAGCTGTCTCCATGAC
  AGTAGGACAAGCCACAACAAAATAGTGCCTTCAACAAGCCTCAAAGACTGCAGCAATGTC
  AGCGTGGACCACACAAGGAATCCAAGCGTGGCAATTCCCCCACTTACACACAATCTTTCT
  GCAGTTGCTCCCAGCATTAATTCTGGAATGGGGACTGAGACTATACCAATTCAGGGTTAC
  AGAGTGGATGAGAAAACTAAGAAGTGTTCTATTCCATTTGTTAAACCGAACAGACATTCC
  CCATCAGGCATTTATAACATTAATGTGACCACATTAGTATCAGGACCTCCCCTGTCAGAT
  GATTCAGGGGCTGATTTGCCTCAAATGGAACACCAGCACTGA
  
  Clone variation with respect to NM_003948.3
  98 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003948 unedited
  CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCAGGCTCCAGGT
  ACTGGGCGCCTTACGAGCTGGGAGGTGGTGCCTCTCACCCAGCTAATTGCTCTCTAGCCCTTGGCCTTCA
  CAGGTGTTGGTGCCTGCCGTGAACGCATTCTGACCTGGGCCGTATCTGTCTCCCAAGACTTTGTGCCTAT
  GGTTGGGGACAGAGTGAGGTCGTTGCCTTGACGACGACAGCATGCGGCCCGTGGTCCTCCTAAGTGTGAG
  CTTGCGGCGGACCGAGGCCCACCTGCCTCCCTGCCTGCTTCGCCCTGGACTCGTGACTGCGTCCGCAGAG
  AATCACAACAGCGCTGGATTTGCTAGTTTGCTAGGCAGCATCTTTGGACCTGCGAACCATATGCATTTCA
  CCTCAAATTTGTTTCCAAGTTGAAAACCTTTGGGTCTTTCTATGCGAACGGATTGAAGAACCGCAAAAGT
  TCTACGGACTTTAAATAAATGGAAAAATTATGAAAACCTGGGTAGTGGAAAATGAGTTATGATGGTGAAT
  GAATGTGTAGAATAAAGATCCTGGAAGATTGGGCCTATATGGAGTTCTAAAATGTGCCAATAGCACAACG
  GGTTAAAGAATGCATGCCGAAAATCCAGTTTCATAGCCACTTAGGCTGGAACCTTGTGGAATCTCTTGGA
  ACGTTGAAAAAAAAACCAATGGCTCATGCCTGGAATTGTGTACCACCATCTTGAGACTGGGACCCTTCCA
  TGAGCATCAGTCACCGAAGTTCAGACTGTCGACAATTATAGAGCGTAGTTGCTGACGCACCTACACGAAA
  TGACCGAGAATCGTATTTCACTTGCGCTATATCGACACTTGATTGTACA
  
  Kinase domain sequence

  >SC323614 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GAAACCTGMGGTTTGGTTGGAGAAGGGAGTTATGGAATGGTGATGAAGTGTAGGAATAAAGATMCTYGAA
  GAATTGTGGCCATAATGAAGTTCTTAGAAAGTGACGATGACAAAATGGTTAAAAAGATTGCAATGCGAGA
  AATCAAGTTACTAAAGCAACTTAGGCATGAAAACTTGGTGAATCTCTTGGAAGTGTGTAAGAAAAAAAAA
  CGATGGTACCTAGTCTTTGAATTTGTTGACCACACAATTCTTGAT
  
• Restriction Sites: Please inquire
• ACCN: NM_003948
• ORF Size: 1482 bp
• Insert Size: 4700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_003948.2, NP_003939.1
• RefSeq Size: 1993
• RefSeq ORF: 1482
• Locus ID: 8999
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the cytoplasm, with lower levels in the nucleus. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.