MEK5 (MAP2K5) (NM_002757) Human Untagged Clone

CAT#: SC323639

MAP2K5 (untagged)-Kinase deficient mutant (K195M) of Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant B

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP2K5
• Synonyms: HsT17454; MAPKK5; MEK5; PRKMK5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002757, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGTGGCTAGCCCTTGGCCCCTTTCCTGCCATGGAGAACCAGGTGCTGGTAATTCGCATCAAGATCC
  CAAATAGTGGCGCGGTGGACTGGACAGTGCACTCCGGGCCGCAGTTACTCTTCAGGGATGTGCTGGATGT
  GATAGGCCAGGTTCTGCCTGAAGCAACAACTACAGCATTTGAATATGAAGATGAAGATGGTGATCGAATT
  ACAGTGAGAAGTGATGAGGAAATGAAGGCAATGCTGTCATATTATTATTCCACAGTAATGGAACAGCAAG
  TAAATGGACAGTTAATAGAGCCTCTGCAGATATTTCCAAGAGCCTGCAAGCCTCCTGGGGAACGGAACAT
  ACATGGCCTGAAGGTGAATACTCGGGCCGGACCCTCTCAACACAGCAGCCCAGCAGTCTCAGATTCACTT
  CCAAGCAATAGCTTAAAGAAGTCTTCTGCTGAACTGAAAAAAATACTAGCCAATGGCCAGATGAATGAAC
  AAGACATACGATATCGGGACACTCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCATATCATGTCCC
  GAGTGGGAAAATATTAGCTGTAAAGGTCATACTACTAGATATTACACTGGAACTTCAGAAGCAAATTATG
  TCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATTTTATGGAGCATTTTTTGTAG
  AAAACAGGATTTCAATATGTACAGAATTCATGGATGGGGGATCTTTGGATGTATATAGGAAAATGCCAGA
  ACATGTCCTTGGAAGAATTGCAGTAGCAGTTGTTAAAGGCCTTACTTATTTGTGGAGTTTAAAGATTTTA
  CATAGAGACGTGAAGCCCTCCAATATGCTAGTAAACACAAGAGGACAGGTTAAGCTGTGTGATTTTGGAG
  TTAGCACTCAGCTGGTGAATTCTATAGCCAAGACGTATGTTGGAACAAATGCTTATATGGCGCCTGAAAG
  GATTTCAGGGGAGCAGTATGGAATTCATTCTGATGTCTGGAGCTTAGGAATCTCTTTTATGGAGATTCAG
  AAAAACCAGGGATCTTTAATGCCTCTCCAGCTTCTGCAGTGCATTGTTGATGAGGATTCGCCCGTCCTTC
  CAGTTGGAGAGTTCTCGGAGCCATTTGTACATTTCATCACTCAGTGTATGCGAAAACAGCCAAAAGAAAG
  GCCAGCACCTGAAGAATTGATGGGCCACCCGTTCATCGTGCAGTTCAATGATGGAAATGCCGCCGTGGTG
  TCCATGTGGGTGTGCCGGGCGCTGGAGGAGAGGCGGAGCCAGCAGGGGCCCCCGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002757 unedited
  ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCGCCGCCGCCGC
  AGCCGCCGCCGGTCCGCGCGGCCTCGGGTGGCCGGAGCTCAGCCTGCGCGCGCCGCGCCCTGTGTCTCCG
  GGTGGGGCAGAAGACTCGCCCCTTGAACCTCCCGCGGGGACTCTCCGTGGTGTGGCGGCCCTGGGGCTCT
  TTCTTAATAGCCCCGGACTGAGTCCCCCTCCAGTCGAGGACCCTCTCCCTAGTCCCACTGACGAACGGGT
  GTGGACAACCTGTGCCCGCTGTGTATTCTCCCCCCAACCCGAGTTCCTTGCCCTGTGCTTGCCCTCCCTA
  TATACCCCACCCCGGGCGGGCAGAGAACTTTTCACCCATACCCGTTTGCGCTTCACCCTCCCGAAACTTT
  TCGGAACCCTCCCGCTAGTTTCCCTGCCGGGCCCTTTTGCCCCGTTTTCCCGGGGGCCCCCCTCCCCGGG
  GAGAAACACCCTAGAACCCCCGACAAGACCTGGGGACGGGCCGGGGGCCCCGGGGGGGTCCTTTCCTGAA
  TCACCCCTTCCCTCTTTTCCTTTCCCCCTTAATCCCCTTTCCCTGGTTTTTTCCCCCTTTTGCCCTGGGG
  CCGGCCCCCCCCCTTTTGCCCCCTTTGGGGACCCCCTCTTAAAACCGGGGGCCAGGGGGGGTTTTCCCTA
  TCCCCCCCGAAGTTGGGCCCCCCTTTAAACCGTGATGGGGCTGGCGGAGCCCCTTGGTGCCCTCTTTTTC
  CCCCCCGGGAGACAACACATGGTGGGGTGGTTATCTCTCCACGAATACACACAATAATTGTGCGCCGCGG
  AGCAGATCAGAGTCAGATCACGCGGGCGCACAATATCTTCTCTGAGAGTGCTGCTGATGTGTATTGACCA
  CGATCTTTGCGTGCTACACACCATCACCATTGTATGTATGGATGAGAACGAGATGGCGCTACCTAGTCCG
  TAAGGCGATCTGCATGATT
  
  Kinase domain sequence

  >SC323639 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GMGATGATGACAAGACTACGATATCGGGACACTCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCAT
  ATCATGTCCCGAGTGGGAAAATATTAGCTGTAATGGTCATACTACTAGATATTACACTGGAACTTCAGAA
  GCAAATTATGTCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATTTTATGGAGCA
  TTTTTTGTAGAAAACAGGATTTCAATATGTACAGAATTCATGGAT
  
• Restriction Sites: Please inquire
• ACCN: NM_002757
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002757.2, NP_002748.1
• RefSeq Size: 2334 bp
• RefSeq ORF: 1317 bp
• Locus ID: 5607
• Cytogenetics: 15q23
• Domains: PB1, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, MAPK signaling pathway, Neurotrophin signaling pathway
• Gene Summary: 'The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (B) has the same N- and C-termini but is shorter compared to isoform A.