MEK5 (MAP2K5) (NM_002757) Human Untagged Clone

CAT#: SC323639

MAP2K5 (untagged)-Kinase deficient mutant (K195M) of Human mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant B


  "NM_002757" in other vectors (6)

Reconstitution Protocol

USD 740.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MAP2K5
Synonyms HsT17454; MAPKK5; MEK5; PRKMK5
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_002757, the custom clone sequence may differ by one or more nucleotides


ATGCTGTGGCTAGCCCTTGGCCCCTTTCCTGCCATGGAGAACCAGGTGCTGGTAATTCGCATCAAGATCC
CAAATAGTGGCGCGGTGGACTGGACAGTGCACTCCGGGCCGCAGTTACTCTTCAGGGATGTGCTGGATGT
GATAGGCCAGGTTCTGCCTGAAGCAACAACTACAGCATTTGAATATGAAGATGAAGATGGTGATCGAATT
ACAGTGAGAAGTGATGAGGAAATGAAGGCAATGCTGTCATATTATTATTCCACAGTAATGGAACAGCAAG
TAAATGGACAGTTAATAGAGCCTCTGCAGATATTTCCAAGAGCCTGCAAGCCTCCTGGGGAACGGAACAT
ACATGGCCTGAAGGTGAATACTCGGGCCGGACCCTCTCAACACAGCAGCCCAGCAGTCTCAGATTCACTT
CCAAGCAATAGCTTAAAGAAGTCTTCTGCTGAACTGAAAAAAATACTAGCCAATGGCCAGATGAATGAAC
AAGACATACGATATCGGGACACTCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCATATCATGTCCC
GAGTGGGAAAATATTAGCTGTAAAGGTCATACTACTAGATATTACACTGGAACTTCAGAAGCAAATTATG
TCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATTTTATGGAGCATTTTTTGTAG
AAAACAGGATTTCAATATGTACAGAATTCATGGATGGGGGATCTTTGGATGTATATAGGAAAATGCCAGA
ACATGTCCTTGGAAGAATTGCAGTAGCAGTTGTTAAAGGCCTTACTTATTTGTGGAGTTTAAAGATTTTA
CATAGAGACGTGAAGCCCTCCAATATGCTAGTAAACACAAGAGGACAGGTTAAGCTGTGTGATTTTGGAG
TTAGCACTCAGCTGGTGAATTCTATAGCCAAGACGTATGTTGGAACAAATGCTTATATGGCGCCTGAAAG
GATTTCAGGGGAGCAGTATGGAATTCATTCTGATGTCTGGAGCTTAGGAATCTCTTTTATGGAGATTCAG
AAAAACCAGGGATCTTTAATGCCTCTCCAGCTTCTGCAGTGCATTGTTGATGAGGATTCGCCCGTCCTTC
CAGTTGGAGAGTTCTCGGAGCCATTTGTACATTTCATCACTCAGTGTATGCGAAAACAGCCAAAAGAAAG
GCCAGCACCTGAAGAATTGATGGGCCACCCGTTCATCGTGCAGTTCAATGATGGAAATGCCGCCGTGGTG
TCCATGTGGGTGTGCCGGGCGCTGGAGGAGAGGCGGAGCCAGCAGGGGCCCCCGTGA


>OriGene 5' read for mutant NM_002757 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCGCCGCCGCCGC
AGCCGCCGCCGGTCCGCGCGGCCTCGGGTGGCCGGAGCTCAGCCTGCGCGCGCCGCGCCCTGTGTCTCCG
GGTGGGGCAGAAGACTCGCCCCTTGAACCTCCCGCGGGGACTCTCCGTGGTGTGGCGGCCCTGGGGCTCT
TTCTTAATAGCCCCGGACTGAGTCCCCCTCCAGTCGAGGACCCTCTCCCTAGTCCCACTGACGAACGGGT
GTGGACAACCTGTGCCCGCTGTGTATTCTCCCCCCAACCCGAGTTCCTTGCCCTGTGCTTGCCCTCCCTA
TATACCCCACCCCGGGCGGGCAGAGAACTTTTCACCCATACCCGTTTGCGCTTCACCCTCCCGAAACTTT
TCGGAACCCTCCCGCTAGTTTCCCTGCCGGGCCCTTTTGCCCCGTTTTCCCGGGGGCCCCCCTCCCCGGG
GAGAAACACCCTAGAACCCCCGACAAGACCTGGGGACGGGCCGGGGGCCCCGGGGGGGTCCTTTCCTGAA
TCACCCCTTCCCTCTTTTCCTTTCCCCCTTAATCCCCTTTCCCTGGTTTTTTCCCCCTTTTGCCCTGGGG
CCGGCCCCCCCCCTTTTGCCCCCTTTGGGGACCCCCTCTTAAAACCGGGGGCCAGGGGGGGTTTTCCCTA
TCCCCCCCGAAGTTGGGCCCCCCTTTAAACCGTGATGGGGCTGGCGGAGCCCCTTGGTGCCCTCTTTTTC
CCCCCCGGGAGACAACACATGGTGGGGTGGTTATCTCTCCACGAATACACACAATAATTGTGCGCCGCGG
AGCAGATCAGAGTCAGATCACGCGGGCGCACAATATCTTCTCTGAGAGTGCTGCTGATGTGTATTGACCA
CGATCTTTGCGTGCTACACACCATCACCATTGTATGTATGGATGAGAACGAGATGGCGCTACCTAGTCCG
TAAGGCGATCTGCATGATT
>SC323639 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GMGATGATGACAAGACTACGATATCGGGACACTCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCAT
ATCATGTCCCGAGTGGGAAAATATTAGCTGTAATGGTCATACTACTAGATATTACACTGGAACTTCAGAA
GCAAATTATGTCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATTTTATGGAGCA
TTTTTTGTAGAAAACAGGATTTCAATATGTACAGAATTCATGGAT
Restriction Sites Please inquire     
ACCN NM_002757
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002757.2, NP_002748.1
RefSeq Size 2334 bp
RefSeq ORF 1317 bp
Locus ID 5607
Cytogenetics 15q23
Domains PB1, pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Gap junction, MAPK signaling pathway, Neurotrophin signaling pathway
Gene Summary 'The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011]'
Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (B) has the same N- and C-termini but is shorter compared to isoform A.

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