Myosin light chain kinase (MYLK) (NM_053027) Human Untagged Clone
CAT#: SC323650
MYLK (untagged)-Kinase deficient mutant (K1493M) of Human myosin light chain kinase (MYLK), transcript variant 3A
"NM_053027" in other vectors (2)
SC323650 is the updated version of SC120194.
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | MYLK |
Synonyms | AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK1; smMLCK |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_053027, the custom clone sequence may differ by one or more nucleotides
ATGGGGGATGTGAAGCTGGTTGCCTCGTCACACATTTCCAAAACCTCCCTCAGTGTGGATCCCTCAAGAG TTGACTCCATGCCCCTGACAGAGGCCCCTGCTTTCATTTTGCCCCCTCGGAACCTCTGCATCAAAGAAGG AGCCACCGCCAAGTTCGAAGGGCGGGTCCGGGGTTACCCAGAGCCCCAGGTGACATGGCACAGAAACGGG CAACCCATCACCAGCGGGGGCCGCTTCCTGCTGGATTGCGGCATCCGGGGGACTTTCAGCCTTGTGATTC ATGCTGTCCATGAGGAGGACAGGGGAAAGTATACCTGTGAAGCCACCAATGGCAGTGGTGCTCGCCAGGT GACAGTGGAGTTGACAGTAGAAGGAAGTTTTGCGAAGCAGCTTGGTCAGCCTGTTGTTTCCAAAACCTTA GGGGATAGATTTTCAGCTCCAGCAGTGGAGACCCGTCCTAGCATCTGGGGGGAGTGCCCACCAAAGTTTG CTACCAAGCTGGGCCGAGTTGTGGTCAAAGAAGGACAGATGGGACGATTCTCCTGCAAGATCACTGGCCG GCCCCAACCGCAGGTCACCTGGCTCAAGGGAAATGTTCCACTGCAGCCGAGTGCCCGTGTGTCTGTGTCT GAGAAGAACGGCATGCAGGTTCTGGAAATCCATGGAGTCAACCAAGATGACGTGGGAGTGTACACGTGCC TGGTGGTGAACGGGTCGGGGAAGGCCTCGATGTCAGCTGAACTTTCCATCCAAGGTTTGGACAGTGCCAA TAGGTCATTTGTGAGAGAAACAAAAGCCACCAATTCAGATGTCAGGAAAGAGGTGACCAATGTAATCTCA AAGGAGTCGAAGCTGGACAGTCTGGAGGCTGCAGCCAAAAGCAAGAACTGCTCCAGCCCCCAGAGAGGTG GCTCCCCACCCTGGGCTGCAAACAGCCAGCCTCAGCCCCCAAGGGAGTCCAAGCTGGAGTCATGCAAGGA CTCGCCCAGAACGGCCCCGCAGACCCCGGTCCTTCAGAAGACTTCCAGCTCCATCACCCTGCAGGCCGCA AGAGTTCAGCCGGAACCAAGAGCACCAGGCCTGGGGGTCCTATCACCTTCTGGAGAAGAGAGGAAGAGGC CAGCTCCTCCCCGTCCAGCCACCTTCCCCACCAGGCAGCCTGGCCTGGGGAGCCAAGATGTTGTGAGCAA GGCTGCTAACAGGAGAATCCCCATGGAGGGCCAGAGGGATTCAGCATTCCCCAAATTTGAGAGCAAGCCC CAAAGCCAGGAGGTCAAGGAAAATCAAACTGTCAAGTTCAGATGTGAAGTTTCCGGGATTCCAAAGCCTG AAGTGGCCTGGTTCCTGGAAGGCACCCCCGTGAGGAGACAGGAAGGCAGCATTGAGGTTTATGAAGATGC TGGCTCCCATTACCTCTGCCTGCTGAAAGCCCGGACCAGGGACAGTGGGACATACAGCTGCACTGCTTCC AACGCCCAAGGCCAGCTGTCCTGTAGCTGGACCCTCCAAGTGGAAAGGCTTGCCGTGATGGAGGTGGCCC CCTCCTTCTCCAGTGTCCTGAAGGACTGCGCTGTTATTGAGGGCCAGGATTTTGTGCTGCAGTGCTCCGT ACGGGGGACCCCAGTGCCCCGGATCACTTGGCTGCTGAATGGGCAGCCCATCCAGTACGCTCGCTCCACC TGCGAGGCCGGCGTGGCTGAGCTCCACATCCAGGATGCCCTGCCGGAGGACCATGGCACCTACACCTGCC TAGCTGAGAATGCCTTGGGGCAGGTGTCCTGCAGCGCCTGGGTCACCGTCCATGAAAAGAAGAGTAGCAG GAAGAGTGAGTACCTTCTGCCTGTGGCTCCCAGCAAGCCCACTGCACCCATCTTCCTGCAGGGCCTCTCT GATCTCAAAGTCATGGATGGAAGCCAGGTCACTATGACTGTCCAAGTGTCAGGGAATCCACCCCCTGAAG TCATCTGGCTGCACAATGGGAATGAGATCCAAGAGTCAGAGGACTTCCACTTTGAACAGAGAGGAACTCA GCACAGCCTTTGTATCCAGGAAGTGTTCCCGGAGGACACGGGCACGTACACCTGCGAGGCCTGGAACAGC GCTGGAGAGGTCCGCACCCAGGCCGTGCTCACGGTACAAGAGCCTCACGATGGCACCCAGCCCTGGTTCA TCAGTAAGCCTCGCTCAGTGACAGCCTCCCTGGGCCAGAGTGTCCTCATCTCCTGCGCCATAGCTGGTGA CCCCTTTCCTACCGTGCACTGGCTCAGAGATGGCAAAGCCCTCTGCAAAGACACTGGCCACTTCGAGGTG CTTCAGAATGAGGACGTGTTCACCCTGGTTCTAAAGAAGGTGCAGCCCTGGCATGCCGGCCAGTATGAGA TCCTGCTCAAGAACCGGGTTGGCGAATGCAGTTGCCAGGTGTCACTGATGCTACAGAACAGCTCTGCCAG AGCCCTTCCACGGGGGAGGGAGCCTGCCAGCTGCGAGGACCTCTGTGGTGGAGGAGTTGGTGCTGATGGT GGTGGTAGTGACCGCTATGGGTCCCTGAGGCCTGGCTGGCCAGCAAGAGGGCAGGGTTGGCTAGAGGAGG AAGACGGCGAGGACGTGCGAGGGGTGCTGAAGAGGCGCGTGGAGACGAGGCAGCACACTGAGGAGGCGAT CCGCCAGCAGGAGGTGGAGCAGCTGGACTTCCGAGACCTCCTGGGGAAGAAGGTGAGTACAAAGACCCTA TCGGAAGACGACCTGAAGGAGATCCCAGCCGAGCAGATGGATTTCCGTGCCAACCTGCAGCGGCAAGTGA AGCCAAAGACTGTGTCTGAGGAAGAGAGGAAGGTGCACAGCCCCCAGCAGGTCGATTTTCGCTCTGTCCT GGCCAAGAAGGGGACTTCCAAGACCCCCGTGCCTGAGAAGGTGCCACCGCCAAAACCTGCCACCCCGGAT TTTCGCTCAGTGCTGGGTGGCAAGAAGAAATTACCAGCAGAGAATGGCAGCAGCAGTGCCGAGACCCTGA ATGCCAAGGCAGTGGAGAGTTCCAAGCCCCTGAGCAATGCACAGCCTTCAGGGCCCTTGAAACCCGTGGG CAACGCCAAGCCTGCTGAGACCCTGAAGCCAATGGGCAACGCCAAGCCTGCCGAGACCCTGAAGCCCATG GGCAATGCCAAGCCTGATGAGAACCTGAAATCCGCTAGCAAAGAAGAACTCAAGAAAGACGTTAAGAATG ATGTGAACTGCAAGAGAGGCCATGCAGGGACCACAGATAATGAAAAGAGATCAGAGAGCCAGGGGACAGC CCCAGCCTTCAAGCAGAAGCTGCAAGATGTTCATGTGGCAGAGGGCAAGAAGCTGCTGCTCCAGTGCCAG GTGTCTTCTGACCCCCCAGCCACCATCATCTGGACGCTGAACGGAAAGACCCTCAAGACCACCAAGTTCA TCATCCTCTCCCAGGAAGGCTCACTCTGCTCCGTCTCCATCGAGAAGGCACTGCCTGAGGACAGAGGCTT ATACAAGTGTGTAGCCAAGAATGACGCTGGCCAGGCGGAGTGCTCCTGCCAAGTCACCGTGGATGATGCT CCAGCCAGTGAGAACACCAAGGCCCCAGAGATGAAATCCCGGAGGCCCAAGAGCTCTCTTCCTCCCGTGC TAGGAACTGAGAGTGATGCGACTGTGAAAAAGAAACCTGCCCCCAAGACACCTCCGAAGGCAGCAATGCC CCCTCAGATCATCCAGTTCCCTGAGGACCAGAAGGTACGCGCAGGAGAGTCAGTGGAGCTGTTTGGCAAA GTGACAGGCACTCAGCCCATCACCTGTACCTGGATGAAGTTCCGAAAGCAGATCCAGGAAAGCGAGCACA TGAAGGTGGAGAACAGCGAGAATGGCAGCAAGCTCACCATCCTGGCCGCGCGCCAGGAGCACTGCGGCTG CTACACACTGCTGGTGGAGAACAAGCTGGGCAGCAGGCAGGCCCAGGTCAACCTCACTGTCGTGGATAAG CCAGACCCCCCAGCTGGCACACCTTGTGCCTCTGACATTCGGAGCTCCTCACTGACCCTGTCCTGGTATG GCTCCTCATATGATGGGGGCAGTGCTGTACAGTCCTACAGCATCGAGATCTGGGACTCAGCCAACAAGAC GTGGAAGGAACTAGCCACATGCCGCAGCACCTCTTTCAACGTCCAGGACCTGCTGCCTGACCACGAATAT AAGTTCCGTGTACGTGCAATCAACGTGTATGGAACCAGTGAGCCAAGCCAGGAGTCTGAACTCACAACGG TAGGAGAGAAACCTGAAGAGCCGAAGGATGAAGTGGAGGTGTCAGATGATGATGAGAAGGAGCCCGAGGT TGATTACCGGACAGTGACAATCAATACTGAACAAAAAGTATCTGACTTCTACGACATTGAGGAGAGATTA GGATCTGGGAAATTTGGACAGGTCTTTCGACTTGTAGAAAAGAAAACTCGAAAAGTCTGGGCAGGGAAGT TCTTCAAGGCATATTCAGCAAAAGAGAAAGAGAATATCCGGCAGGAGATTAGCATCATGAACTGCCTCCA CCACCCTAAGCTGGTCCAGTGTGTGGATGCCTTTGAAGAAAAGGCCAACATCGTCATGGTCCTGGAGATC GTGTCAGGAGGGGAGCTGTTTGAGCGCATCATTGACGAGGACTTTGAGCTGACGGAGCGTGAGTGCATCA AGTACATGCGGCAGATCTCGGAGGGAGTGGAGTACATCCACAAGCAGGGCATCGTGCACCTGGACCTCAA GCCGGAGAACATCATGTGTGTCAACAAGACGGGCACCAGGATCAAGCTCATCGACTTTGGTCTGGCCAGG AGGCTGGAGAATGCGGGGTCTCTGAAGGTCCTCTTTGGCACCCCAGAATTTGTGGCTCCTGAAGTGATCA ACTATGAGCCCATCGGCTACGCCACAGACATGTGGAGCATCGGGGTCATCTGCTACATCCTAAACCGCCT GGACTGCACGCAGTGCCTTCAGCATCCATGGCTAATGAAAGATACCAAGAACATGGAGGCCAAGAAACTC TCCAAGGACCGGATGAAGAAGTACATGGCAAGAAGGAAATGGCAGAAAACGGGCAATGCTGTGAGAGCCA TTGGAAGACTGTCCTCTATGGCAATGATCTCAGGGCTCAGTGGCAGGAAATCCTCAACAGGGTCACCAAC CAGCCCGCTCAATGCAGAAAAACTAGAATCTGAAGAAGATGTGTCCCAAGCTTTCCTTGAGGCTGTTGCT GAGGAAAAGCCTCATGTAAAACCCTATTTCTCTAAGACCATTCGCGATTTAGAAGTTGTGGAGGGAAGTG CTGCTAGATTTGACTGCAAGATTGAAGGATACCCAGACCCCGAGGTTGTCTGGTTCAAAGATGACCAGTC AATCAGGGAGTCCCGCCACTTCCAGATAGACTACGATGAGGACGGGAACTGCTCTTTAATTATTAGTGAT GTTTGCGGGGATGACGATGCCAAGTACACCTGCAAGGCTGTCAACAGTCTTGGAGAAGCCACCTGCACAG CAGAGCTCATTGTGGAAACGATGGAGGAAGGTGAAGGGGAAGGGGAAGAGGAAGAAGAGTGA >OriGene 5' read for mutant NM_053027 unedited
ACCGCCCGTTGAGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGATGTGAAGCT GGTTGCCTCGTCGCACATTTCCAAAACCTCCCTCAGTGTGGATCACTCAAGAGTTGACTCCATGCCCCTG ACAGAGGCCCCTGCTTTCATTTTGCCCCCTCGGAACCTCTGCATCAAAGAAGGAGCCACCCGCCAAGTTC GAAGGGCGGGTCCGGGGTTCCCAGAACCCCAGGTGACATGGCACAAAACCGGGAACCCGGGGGGGGTGTG TGGGCCGCTTCCGGTGGATGGCGCCTCCGGGGGACTTAACCTTTGATTTTTGGGGGTGGGGAG >SC323650 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GAAAGTWCTGACTTCTACGACATTGAGGAGAGATTAGGATCTGGGAAATTTGGACAGGTCTTTCGACTTG TAGAAAAGAAAACTCGAAAAGTCTGGGCAGGGATGTTCTTCAAGGCATATTCAGCAAAAGAGAAAGAGAA TATCCGGCAGGAGATTAGCATCATGAACTGCCTCCACCACCCTAAGCTGGTCCAGTGTGTGGATGCCTTT GAAGAAAAGGCCAACATCGTCATGGTCCTGGAGATCGTGTCAGGA |
Restriction Sites | Please inquire |
ACCN | NM_053027 |
Insert Size | 5600 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053027.2, NP_444255.2 |
RefSeq Size | 7699 bp |
RefSeq ORF | 5592 bp |
Locus ID | 4638 |
Cytogenetics | 3q21.1 |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | Calcium signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Vascular smooth muscle contraction |
Gene Summary | 'This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008]' Transcript Variant: This variant (3A) does not utilize exon 30, compared to variant 1, resulting in a shorter protein (isoform 3A), compared to isoform 1. |
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