ALPK1 (NM_025144) Human Untagged Clone

CAT#: SC323654

ALPK1 (untagged)-Kinase deficient mutant (K1067M) of Human alpha-kinase 1 (ALPK1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALPK1
• Synonyms: 8430410J10Rik; LAK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323654 sequence for NM_025144 edited (data generated by NextGen Sequencing)
  ATGAATAATCAAAAAGTGGTAGCTGTGCTACTGCAAGAGTGCAAGCAAGTGCTGGATCAG
  CTCTTGTTGGAAGCGCCAGATGTGTCGGAAGAGGACAAGAGCGAGGACCAGCGCTGCAGA
  GCTTTACTCCCCAGCGAGTTAAGGACCCTGATCCAGGAGGCAAAGGAAATGAAGTGGCCC
  TTCGTGCCTGAAAAGTGGCAGTACAAACAAGCCGTGGGCCCAGAGGACAAAACAAACCTG
  AAGGATGTGATTGGCGCCGGGTTGCAGCAGTTACTGGCGTCCCTGAGGGCCTCCATCCTC
  GCTCGGGACTGTGCGGCTGCGGCGGCTATTGTGTTCTTGGTGGACCGGTTCCTGTATGGG
  CTCGACGTCTCTGGAAAACTTCTGCAGGTCGCCAAAGGTCTCCACAAGTTGCAGCCAGCC
  ACGCCAATTGCCCCGCAGGTGGTTATTCGCCAAGCCCGAATCTCCGTGAACTCAGGAAAA
  CTTTTAAAAGCAGAGTATATTCTGAGCAGTCTAATAAGCAACAATGGAGCAACGGGTACC
  TGGCTGTACAGAAATGAAAGTGACAAGGTCCTGGTGCAGTCGGTCTGTATACAGATCAGA
  GGGCAGATTCTGCAAAAGCTGGGGATGTGGTACGAAGCAGCAGAGTTAATATGGGCCTCC
  ATTGTAGGATATTTGGCACTTCCTCAGCCGGATAAAAAGGGCCTCTCCACGTCGCTAGGT
  ATACTGGCAGACATCTTTGTTTCCATGAGCAAGAACGATTATGAAAAGTTTAAAAACAAT
  CCACAAATTAATTTGAGCCTGCTGAAGGAGTTTGACCACCATTTGCTGTCCGCTGCAGAA
  GCCTGCAAGCTGGCAGCTGCCTTCAGTGCCTATACGCCGCTCTTCGTGCTCACAGCTGTG
  AATATCCGTGGCACGTGTTTATTGTCCTACAGTAGTTCAAATGACTGTCCTCCAGAATTG
  AAAAACTTACATCTGTGTGAAGCCAAAGAGGCCTTTGAGATTGGCCTCCTCACCAAGAGA
  GATGATGAGCCTGTTACTGGAAAACAGGAGCTTCACAGCTTTGTCAAAGCTGCTTTCGGT
  CTCACCACAGTGCACAGAAGGCTCCATGGGGAGACAGGGACGGTCCATGCAGCAAGTCAG
  CTCTGTAAGGAAGCAATGGGGAAGCTGTACAATTTCAGCACTTCCTCCAGAAGTCAGGAC
  AGAGAAGCTCTGTCTCAAGAAGTTATGTCTGTGATTGCCCAGGTGAAGGAACATTTACAA
  GTTCAAAGCTTCTCAAATGTAGATGACAGATCTTATGTTCCCGAGAGTTTCGAGTGCAGG
  TTGGATAAACTTATCTTGCATGGGCAAGGGGATTTCCAAAAAATCCTTGACACCTATTCA
  CAGCACCATACTTCGGTGTGTGAAGTATTTGAAAGTGATTGTGGAAACAACAAAAATGAA
  CAGAAAGATGCAAAAACAGGAGTCTGCATCACTGCTCTAAAAACAGAAATAAAAAACATA
  GATACTGTGAGTACTACTCAAGAAAAGCCACATTGTCAAAGAGACACAGGAATATCTTCC
  TCCCTAATGGGTAAGAATGTTCAGAGGGAACTCAGAAGGGGAGGAAGGAGAAACTGGACC
  CATTCTGATGCATTTCGAGTCTCCTTGGATCAAGATGTGGAGACTGAGACTGAGCCATCG
  GACTACAGCAATGATGAGGGAGCTGTTTTCAACAAGTCTCTGAGTGGCAGCCAGACTTCC
  AGTGCTTGGAGCAACTTATCAGGGTTTAGTTCCTCTGCAAGCTGGGAGGAAGTGAATTAT
  CACGTTGACGACAGGTCAGCCAGAAAAGAGCCTGGCAAAGAACATCTGGTGGACACTCAG
  TGTTCCACTGCCTTGTCTGAGGAGCTAGAGAATGACAGGGAAGGCAGAGCTATGCATTCA
  TTGCGTTCACAGCTTCATGATCTCTCTCTTCAGGAACCCAACAATGACAATTTGGAGCCT
  TCTCAAAATCAGCCACAGCAACAGATGCCCTTGACACCCTTCTCGCCTCATAATACCCCA
  GGCATTTTCTTGGCCCCTGGTGCAGGGCTTCTAGAAGGAGCTCCAGAAGGTATCCAGGAA
  GTCAGAAACATGGGACCCAGAAATACTTCTGCTCACTCCAGACCCTCATATCGTTCTGCT
  TCTTGGTCTTCTGATTCTGGTAGGCCCAAGAATATAGGCACACATCCTTCAGTCCAAAAA
  GAAGAAGCCTTTGAAATAATTGTTGAGTTTCCAGAAACCAACTGCGATGTCAAAGACAGG
  CAGGGGAAAGAGCAGGGAGAAGAAATTAGTGAAAGAGGCGCAGGCCCTACATTTAAAGCT
  AGTCCCTCCTGGGTTGACCCAGAAGGAGAAACAGCAGAAAGCACTGAAGATGCACCCTTA
  GACTTTCACAGGGTCCTGCACAATTCTCTGGGAAACATTTCCATGCTGCCATGTAGCTCC
  TTCACCCCTAATTGGCCTGTTCAAAATCCTGACTCCAGAAAAAGTGGTGGCCCAGTCGCA
  GAGCAGGGCATCGACCCTGATGCCTCCACAGTGGATGAGGAGGGGCAACTGCTCGACAGC
  ACGGATGTTCCCTGCACAAATGGGCACGGCTCTCATAGACTGTGCATTCTGAGACAGCCG
  CCTGGTCAGAGGGCGGAGACCCCCAATTCCTCTGTAAGCGGTAACATCCTCTTCCCTGTC
  CTCAGCGAGGACTGCACTACCACAGAGGAAGGAAATCAGCCTGGAAACATGCTAAACTGC
  AGCCAGAACTCCAGCTCATCCTCAGTGTGGTGGCTGAAATCACCTGCATTTTCCAGTGGT
  TCTTCTGAGGGGGACAGCCCTTGGTCCTATCTGAATTCCAGTGGGAGTTCTTGGGTTTCA
  TTGCCGGGAAAGATGAGGAAAGAGATCCTTGAGGCTCGCACCTTGCAACCTGATGACTTT
  GAAAAGCTGTTGGCAGGAGTGAGGCATGATTGGCTGTTTCAGAGACTAGAGAATACGGGG
  GTTTTTAAGCCCAGTCAACTCCACCGAGCACATAGTGCTCTTTTGTTAAAATATTCAAAA
  AAATCTGAACTGTGGACGGCCCAGGAAACTATTGTCTATTTGGGGGACTACTTGACTGTG
  AAGAAAAAAGGCAGACAAAGAAATGCTTTTTGGGTTCATCATCTTCATCAAGAAGAAATT
  CTGGGGAGGTATGTTGGGAAAGACTATAAGGAGCAGAAGGGGCTCTGGCACCACTTCACT
  GATGTGGAGCGGCAGATGACCGCACAGCACTATGTGACAGAATTTAACAAGAGACTCTAT
  GAACAAAACATTCCCACCCAGATATTCTACATCCCATCCACAATACTACTGATTTTAGAG
  GACAAGACAATAAAGGGATGTATCAGTGTGGAGCCTTACATACTGGGAGAATTTGTAAAA
  TTGTCAAATAACACGAAAGTGGTGAAAACAGAATACAAAGCCACAGAATATGGCTTGGCC
  TATGGCCATTTTTCTTATGAGTTTTCTAATCATAGAGATGTTGTGGTCGATTTACAAGGT
  TGGGTAACCGGTAATGGAAAAGGACTCATCTACCTCACAGATCCCCAGATTCACTCCGTT
  GATCAGAAAGTTTTCACTACCAATTTTGGAAAGAGAGGAATTTTTTACTTCTTTAATAAC
  CAGCATGTGGAATGTAATGAAATCTGCCATCGTCTTTCTTTGACTAGACCTTCAATGGAG
  AAACCATGCACATAG
  
  Clone variation with respect to NM_025144.3
  1694 g=>a;1925 a=>g;2109 t=>c;2196 g=>a;2582 t=>c;3252 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_025144 unedited
  ACCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG
  AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCTCATTCCTCAAA
  GATGCTGCAAATTCTAATAAATTCCCAGGTAAGGAGAGGTTGGCCTGGCTTTTTTTGTTTGTTTGTTTGT
  TTGTTTTGTTTTGTTTTGTTTTTGTTTTTTCAGACAGGGTCTAACTCTATTGTCCTGGATGGAGTGCAGT
  GGCACAATCATAATTCACTGCGACCTCAAACTCTTGGACACAAGAAATCCTCCCTCTCAGCATCCTGAGT
  AGCTAAGACTATAGGTAATTGATCACCCTAGACCCAGGGACACCCAATTCATCGTAATCATCATGAATAA
  TCAAAAAGTGGTAGCTGTGCTACTGCAAGAGTGCAAGCAAGTGCTGGATCAGCTCTTGTTGGAAGCGCCA
  GATGTGTCGGAGAGGACAAGAGCGAGGACCAGCGCTGCAGAGCTTTACTCCCAGCGAGTTAAGGACCCTG
  ATCCAGGAGCAAGGAAATGAGTGGCCCTTCGTGCTTGAAAGTGCAGTACAAACAGCGGTGGCCAAAGGAC
  AAACAAACTGAGATGTGATTGCGCGGTTGCAGCAGTTACTGAATTAGGTGCAAAGAGTTATTGCTCAGGA
  AGACAAACAGTATGGTGAATTGACTTACGCTTTCTTATCCACTCAATTTCCATCGGAGCACAATGGAAAC
  TTGAAAACAGGCTCTAGGCCTCACTTCCTTGACTGGCGGTCGGCATTGTTCTGGAACGTTCTGTAGCTCA
  CTCTGACTTCAGGTCAGTTCCATTGGCACCCACGCAATTGGCAGT
  
  Kinase domain sequence

  >SC323654 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CMKTGAGAAAYGCAGACAAAGAAATGCTTTTTGGGTTCATCATCTTCATCAAGAAGAAATTCTGGGGAGG
  TATGTTGGGATGGACTATAAGGAGCAGAAGGGGCTCTGGCACCACTTCACTGATGTGGAGCGGCAGATGA
  CCGCACAGCACTATGTGACAGAATTTAACAAGAGACTCTATGAACAAAACATTCCCACCCAGATATTCTA
  CATCCCATCCACAATACTACTGATTTTAGAGGACAAGACAATAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_025144
• ORF Size: 3735 bp
• Insert Size: 5100
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_025144.2, NP_079420.2
• RefSeq Size: 4281
• RefSeq ORF: 3735
• Locus ID: 80216
• Domains: MHCK_EF2_kinase
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes an alpha kinase. Mice which were homozygous for disrupted copies of this gene exhibited coordination defects (PMID: 21208416). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein (isoform 1).