ULK3 (NM_001099436) Human Untagged Clone

CAT#: SC323667

ULK3 (untagged)-Kinase deficient mutant (K87M) of Human unc-51-like kinase 3 (C. elegans) (ULK3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ULK3
• Synonyms: DKFZp434C131; FLJ90566
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323667 sequence for NM_001099436 edited (data generated by NextGen Sequencing)
  ATGGCGGGGCCCGGCTGGGGTCCCCCGCGCCTGGACGGCTTCATCCTCACCGAGCGCCTG
  GGCAGCGGCACGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGACACTCGTGAAGTG
  GTAGCCATAATGTGTGTAGCCAAGAAAAGTCTGAACAAGGCATCGGTGGAGAACCTCCTC
  ACGGAGATTGAGATCCTCAAGGGCATTCGACATCCCCACATTGTGCAGCTGAAAGACTTT
  CAGTGGGACAGTGACAATATCTACCTCATCATGGAGTTTTGCGCAGGGGGCGACCTGTCT
  CGCTTCATCCATACCCGCAGGATTCTGCCTGAGAAGGTGGCGCGTGTCTTCATGCAGCAA
  TTAGCTAGCGCCCTGCAATTCCTGCATGAACGGAATATCTCTCACCTGGATCTGAAGCCA
  CAGAACATTCTACTGAGCTCCTTGGAGAAGCCCCACCTAAAACTGGCAGACTTTGGTTTC
  GCACAACACATGTCCCCGTGGGATGAGAAGCACGTGCTCCGTGGCTCCCCCCTCTACATG
  GCCCCCGAGATGGTGTGCCAGCGGCAGTATGACGCCCGCGTGGACCTCTGGTCCATGGGG
  GTCATCCTGTATGAAGCCCTCTTCGGGCAGCCCCCCTTTGCCTCCAGGTCGTTCTCGGAG
  CTGGAAGAGAAGATCCGTAGCAACCGGGTCATCGAGCTCCCCTTGCGGCCCCTGCTCTCC
  CGAGACTGCCGGGACCTACTGCAGCGGCTCCTGGAGCGGGACCCCAGCCGTCGCATCTCC
  TTCCAGGACTTTTTTGCGCACCCCTGGGTGGACCTGGAGCACATGCCCAGTGGGGAGAGT
  CTGGGGCGAGCAACCGCCCTGGTGGTGCAGGCTGTGAAGAAAGACCAGGAGGGGGATTCA
  GCAGCTGCCTTATCACTCTACTGCAAGGCTCTGGACTTCTTTGTACCTGCCCTGCACTAT
  GAAGTGGATGCCCAGCGGAAGGAGGCAATTAAGGCAAAGGTGGGGCAGTACGTGTCCCGG
  GCTGAGGAGCTCAAGGCCATCGTCTCCTCTTCCAATCAGGCCCTGCTGAGGCAGGGGACC
  TCTGCCCGAGACCTGCTCAGAGAGATGGCCCGGGACAAGCCACGCCTCCTAGCTGCCCTG
  GAAGTGGCTTCAGCTGCCATGGCCAAGGAGGAGGCCGCCGGCGGGGAGCAGGATGCCCTG
  GACCTGTACCAGCACAGCCTGGGGGAGCTACTGCTGTTGCTGGCAGCGGAGCCCCCGGGC
  CGGAGGCGGGAGCTGCTTCACACTGAGGTTCAGAACCTCATGGCCCGAGCTGAATACTTG
  AAGGAGCAGGTCAAGATGAGGGAATCTCGCTGGGAAGCTGACACCCTGGACAAAGAGGGA
  CTGTCGGAATCTGTTCGTAGCTCTTGCACCCTTCAGTGA
  
  Clone variation with respect to NM_001099436.1
  131 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_001099436 unedited
  CCCGCCCGTCCCGCAACGGGCGGTAGGCGCTGTACGGTTGGGAGGTTCATATAAGCAGAGCTCGTTTAGT
  GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCGCGCCTGGAC
  GGCTTCATCCTCACCGAGCGCCTGGGCAGCGGCACGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGG
  ACACTCGTGAAGTGGTAGCCATAATGTGTGTAGCCAAGAAAAGTCTGAACAAGGCATCGGTGGAGAACCT
  CCTCACGGAGATTGAGATCCTCAAGGGCATTCGACATCCCCACATTGTGCAGCTGAAAGACTTTCAGTGG
  GACAGTGACAATATCTACCTCATCATGGGAGTTTTGCGCAGGGGGCGACCCTGTCTCGCTTCATCCCATA
  CCCCGCAGGATTTCTGCCTGAAAAGGTGCCGCGGTGTCTTCATGCAGCATTTAGCTAGCCGCCCCTGCAT
  TCCTGCATGAAACGGAATTATCCTCTCACCTGATCCGGAGCCCCAGAACATCTACTGACCTCCTGGAAAA
  GCCCACCTAAAACTGGCAAACTTTGGTTTGCAACACACAGGTCCCTTGGTAGAAGCCTTCCCCGGGTCTC
  CCCTCTAATGCCCCAATTGGTGCCACGCGATTAGACCCCTTGACTTTGTCCAGGGGGCTCTCGTGTAAGC
  CTTTGGCAGCCCTTGCCCAGTGTCTCTGACTGAAGAATCTGAAACGGTATAGTCCTTGGCCTGTTCGAAT
  CGCACTCGACGCCTGCGGACAGCGTATATTGATTGACTGACGATCCTTGGACTGCAACCGTTGACGA
  
  Kinase domain sequence

  >SC323667 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CTCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCGCGCCTGGACGGCTTCAT
  CCTCACCGAGCGCCTGGGCAGCGGCACGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGACACTCGT
  GAAGTGGTAGCCATAATGTGTGTAGCCAAGAAAAGTCTGAACAAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001099436
• ORF Size: 1419 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_001099436.1, NP_001092906.1
• RefSeq Size: 2635
• RefSeq ORF: 1419
• Locus ID: 25989
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: mTOR signaling pathway, Regulation of autophagy