LYN (NM_002350) Human Untagged Clone

CAT#: SC323673

LYN (untagged)-Kinase deficient mutant (K275M) of Human v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LYN
• Synonyms: JTK8; p53Lyn; p56Lyn
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002350 edited
  GCCACCCCCGGCCCCGCGCCAGCAGCCCCTCGCCGCGCGTCCAGCGTTCCCGGCCAGCAG
  CCTCCCCATACGCAGGTCCTGCTGGGCCGCCCCGTCGCGCCCCCCACTCTGAACTCAAGT
  CACCGTGGAGCTCCGCCGCCCCGAAACTTTCACCGCGAGCGGGAAATATGGGATGTATAA
  AATCAAAAGGGAAAGACAGCTTGAGTGACGATGGAGTAGATTTGAAGACTCAACCAGTAC
  GTAATACTGAAAGAACTATTTATGTGAGAGATCCAACGTCCAATAAACAGCAAAGGCCAG
  TTCCAGAATCTCAGCTTTTACCTGGACAGAGGTTTCAAACTAAAGATCCAGAGGAACAAG
  GAGACATTGTGGTAGCCTTGTACCCCTATGATGGCATCCACCCGGACGACTTGTCTTTCA
  AGAAAGGAGAGAAGATGAAAGTCCTGGAGGAGCATGGAGAATGGTGGAAAGCAAAGTCCC
  TTTTAACAAAAAAAGAAGGCTTCATCCCCAGCAACTATGTGGCCAAACTCAACACCTTAG
  AAACAGAAGAGTGGTTTTTCAAGGATATAACCAGGAAGGACGCAGAAAGGCAGCTTTTGG
  CACCAGGAAATAGCGCTGGAGCTTTCCTTATTAGAGAAAGTGAAACATTAAAAGGAAGCT
  TCTCTCTGTCTGTCAGAGACTTTGACCCTGTGCATGGTGATGTTATTAAGCACTACAAAA
  TTAGAAGTCTGGATAATGGGGGCTATTACATCTCTCCACGAATCACTTTTCCCTGTATCA
  GCGACATGATTAAACATTACCAAAAGCAGGCAGATGGCTTGTGCAGAAGATTGGAGAAGG
  CTTGTATTAGTCCCAAGCCACAGAAGCCATGGGATAAAGATGCCTGGGAGATCCCCCGGG
  AGTCCATCAAGTTGGTGAAAAGGCTTGGCGCTGGGCAGTTTGGGGAAGTCTGGATGGGTT
  ACTATAACAACAGTACCAAGGTGGCTGTGATGACCCTGAAGCCAGGAACTATGTCTGTGC
  AAGCCTTCCTGGAAGAAGCCAACCTCATGAAGACCCTGCAGCATGACAAGCTCGTGAGGC
  TCTACGCTGTGGTCACCAGGGAGGAGCCCATTTACATCATCACCGAGTACATGGCCAAGG
  GCAGTTTGCTGGATTTCCTGAAGAGCGATGAAGGTGGCAAAGTGCTGCTTCCAAAGCTCA
  TTGACTTTTCTGCTCAGATTGCAGAGGGAATGGCATACATCGAGCGGAAGAACTACATTC
  ACCGGGACCTGCGAGCAGCTAATGTTCTGGTCTCCGAGTCACTCATGTGCAAAATTGCAG
  ATTTTGGCCTTGCTAGAGTAATTGAAGATAATGAGTACACAGCAAGGGAAGGTGCTAAGT
  TCCCTATTAAGTGGACGGCTCCAGAAGCAATCAACTTTGGATGTTTCACTATTAAGTCTG
  ATGTGTGGTCCTTTGGAATCCTCCTATACGAAATTGTCACCTATGGGAAAATTCCCTACC
  CAGGGAGAACTAATGCCGACGTGATGACCGCCCTGTCCCAGGGCTACAGGATGCCCCGTG
  TGGAGAACTGCCCAGATGAGCTCTATGACATTATGAAAATGTGCTGGAAAGAAAAGGCAG
  AAGAGAGACCAACGTTTGACTACTTACAGAGCGTCCTGGATGATTTCTACACAGCCACGG
  AAGGGCAATACCAGCAGCAGCCTTAGAGCACAGGGAGACCCGTCCATTTGGCAGGGGTGG
  CTGCCTCATTTAGAGAGGAAAAGTAACCATCACTGGTTGCACTTATGATTTCATGTGCGG
  GGATCATCTGCCGTGCCTGGATCCTGAAATAGAGGCTAAATTACTCAGGAAGAACACCCT
  CTAAATGGGAAAGTATTCTGTACTCTTAGATGGATTCTCCACTCAGTTGCAACTTGGACT
  TGTCCTCAGCAGCTGGTAATCTTGCTCTGCTTGACAACATCTGAGTGCAGCCGTTTGAGA
  AGAAAACATCTATTCTCTCCAAAAATGCACCCAACTAGCTCTATGTTTACAAATGGACAT
  AGGACTCAAAGTTTCAGAGACCATTGCAATGAATCCCCAATAATTGCAGAACTAAACTCA
  TTTATAAAGCTAAAATAACCGGATATATACATAGCATGACATTTCTTTGTGCTTTGGCTT
  ACTTGTTTAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002350 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCACCCCCGGCCCCG
  CGCCAGCAGCCCCTCGCCGCGCGTCCAGCGTTCCCGGCCAGCAGCCTCCCCATACGCAGGTCCTGCTGGG
  CCGCCCCGTCGCGCCCCCCACTCTGAACTCAAGTCACCGTGGAGCTCCGCCGCCCCGAAACTTTCACCGC
  GAGCGGGAAATATGGGATGTATAAAATCAAAAGGGAAAGACAGCTTTGAGTGACGATGGAGTAGATTTGA
  AGACTCAACCAGTACGTAAATACTGAAAAGAAACTATTTTATGTTGAAGAAAATCAAACGTCCCAAATAA
  AACAGCAAAAGGCCCAGTTTCAGAGATTCTCAGGCTTTTTACCCTGGGAACAGGAAGGTTTCAAAACTTA
  AAGATATCCCGAGAGGAACCAAGGGAAACTTTGGGGTAAGGCTTGTGAACCCCTATTGAGTGGGACTCCA
  CCCCCGAGCGATTTTGCCTTTCCAGGAAGGAAGAAAGATGAAGTCTCTTGGAGGGACAGGGGAAAGGGGG
  AAGAGCAAATTCCCTTTACACAAAAGAGAGGGTTTCTCCCACAGCCTATGGGCCAAACTACACCCCTTAA
  AACAGAGAGTGTTTTTAGGGATATCCCCAAGAGACGCAAGGGCCTTTTGGACACGAGAAATAGCTGTGAG
  CTCTCATATGAGAATGGACACTTAAGAGGCTCTCTCGTGCTGCAGAACTTAGACCTGTCGATGAGGTTTA
  GACACTCAATTATATCTAGATAGGCGCTAATCTTCCGAAACACTTCGTACCCGGTTATATTTTCAAACGC
  AATGTGTGCAAAATGGAAAGACGGTTA
  
  Kinase domain sequence

  >SC323673 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  ARCTGGGRACCCCGGGAGTCATCAAGTTGGTGAAAAGGCTTGGCGCTGGGCAGTTTGGGGAAGTCTGGAT
  GGGTTACTATAACAACAGTACCAAGGTGGCTGTGATGACCCTGAAGCCAGGAACTATGTCTGTGCAAGCC
  TTCCTGGAAGAAGCCAACCTCATGAAGACCCTGCAGCATGACAAGCTCGTGAGGCTCTACGCTGTGGTCA
  CCAGGGAGGAGCCCATTTACATCATCACCGAGTACATGGCCAAGG
  
• Restriction Sites: Please inquire
• ACCN: NM_002350
• Insert Size: 2200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002350.1, NP_002341.1
• RefSeq Size: 2298 bp
• RefSeq ORF: 1539 bp
• Locus ID: 4067
• Cytogenetics: 8q12.1
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: B cell receptor signaling pathway, Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Long-term depression
• Gene Summary: 'This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (1) encodes the longer isoform (A).