POLR3H (NM_001018050) Human Untagged Clone

CAT#: SC323888

POLR3H (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide H (22.9kD) (POLR3H), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR3H
• Synonyms: C25; RPC8; RPC22.9
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001018050.1 CGGGGTCTCGCGGCGCGGGCGCGCACCCGGAGCTGTGGACGGAGAGTGCCTCCCTCTGGC
  CTCAGTTTCCTCATGTTGTAGTAGCGGAGCATGGCCCGGACCGGGCCCCCGAGACCGCCC
  CGTGCAACCTCACCGCCAGCCTGGGGGCCTCAGCGACTGGGACGGGACCAAGGGGCTCGG
  GGATTCTCCCTGCCCCCGGCCCTGGTGCGTGACTGACCCTCCTGTTCCCAGAGCCCCCAG
  CGCAGGCCGGGATGTTCGTCCTGGTGGAAATGGTGGACACCGTCCGGATCCCCCCTTGGC
  AGTTTGAGAGGAAGCTCAACGACTCCATTGCCGAGGAGCTGAACAAGAAGTTGGCCAACA
  AGGTCGTGTACAACGTGGGACTCTGCATTTGTCTGTTTGATATCACCAAACTGGAGGATG
  CCTATGTATTCCCTGGGGATGGCGCATCACACACCAAAGTCCATTTTCGCTGCGTGGTGT
  TTCATCCATTCCTAGATGAGATTCTCATTGGGAAGATCAAAGGCTGCAGCCCAGAAGGAG
  TGCACGTCTCTCTAGGCTTCTTCGATGACATTCTCATCCCCCCAGAGTCACTGCAGCAGC
  CAGCCAAGTTCGACGAAGCGGAGCAGGTGTGGGTGTGGGAGTACGAGACGGAGGAAGGAG
  CACACGACCTCTACATGGACACCGGCGAGGAGATCCGCTTCCGGGTGGTGGACGAGAGCT
  TTGTTGACACGTCCCCCACAGGGCCCAGCTCAGCAGATGCCACCACTTCCAGTGAGGAGC
  TGCCAAAGAAGGAGGCTCCGTACACGCTTGTGGGATCCATCAGTGAGCCAGGCCTGGGCC
  TTCTCTCCTGGTGGACCAGCAACTAGCCCTGGGGCTGGACAGTGGACCCTACCAGCCTGC
  GGGAAGGTGGTATGGCCGGCTGTGAAGACAACAGCAGCTGAGGCCGATGCTAAGGAGATA
  GTGTCTCGAGCTGGACAGTGAGGCAAGGCCAGGAGTGCCCACCAGCTTGCCCCGCTCCTC
  CACCATCTAGGCCCTGCTCCTCCTTCACTGCAGTGATGGATCCATCTGTGGCTGTGGCTG
  TTGGGCCTCCACTGACCTTGACTGAAAAGACCCTTCCTGGACCCTGGACTTGACAACAAA
  CGGCACAGACACCAATCACTCACAGAACAGTCACTGGTTTTGGATTCTTGTTTTCCTTGC
  AAGAACAAAAGGTTCTCTAGAATCCTTCAGCCACCAATGAAATGATCAGGCAGGAGAAAA
  ACTTTAGATGGGAGTCTTGTCATCAAAAATAAATACAGTTGCATGGAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001018050
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001018050.1, NP_001018060.1
• RefSeq Size: 4465 bp
• RefSeq ORF: 615 bp
• Locus ID: 171568
• Cytogenetics: 22q13.2
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 3, 5, and 6 encode the same isoform (a).