RUFY1 (NM_001040451) Human Untagged Clone

CAT#: SC324310

RUFY1 (untagged)-Human RUN and FYVE domain containing 1 (RUFY1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RUFY1
• Synonyms: RABIP4; ZFYVE12
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001040451.1 CTGGGAGAGGCCTCTGGAGCAGGAGGCCCAGTGGCTCTTCTGACCCAAGGCCCCGCCGTC
  CAGCTTCTAAGTGCCAGATGATGGAGGAGCGTGCCAACCTGATGCACATGATGAAACTCA
  GCATCAAGGTGTTGCTCCAGTCGGCTCTGAGCCTGGGCCGCAGCCTGGATGCGGACCATG
  CCCCCTTGCAGCAGTTCTTTGTAGTGATGGAGCACTGCCTCAAACATGGGCTGAAAGTTA
  AGAAGAGTTTTATTGGCCAAAATAAATCATTCTTTGGTCCTTTGGAGCTGGTGGAGAAAC
  TTTGTCCAGAAGCATCAGATATAGCGACTAGTGTCAGAAATCTTCCAGAATTAAAGACAG
  CTGTGGGAAGAGGCCGAGCGTGGCTTTATCTTGCACTCATGCAAAAGAAACTGGCAGATT
  ATCTGAAAGTGCTTATAGACAATAAACATCTCTTAAGCGAGTTCTATGAGCCTGAGGCTT
  TAATGATGGAGGAAGAAGGGATGGTGATTGTTGGTCTGCTGGTGGGACTCAATGTTCTCG
  ATGCCAATCTCTGCTTGAAAGGAGAAGACTTGGATTCTCAGGTTGGAGTAATAGATTTTT
  CCCTCTACCTTAAGGATGTGCAGGATCTTGATGGTGGCAAGGAGCATGAAAGAATTACTG
  ATGTCCTTGATCAAAAAAATTATGTGGAAGAACTTAACCGGCACTTGAGCTGCACAGTTG
  GGGATCTTCAAACCAAGATAGATGGCTTGGAAAAGACTAACTCAAAGCTTCAAGAAGAGC
  TTTCAGCTGCAACAGACCGAATTTGCTCACTTCAAGAAGAACAGCAGCAGTTAAGAGAAC
  AAAATGAATTAATTCGAGAAAGAAGTGAAAAGAGTGTAGAGATAACAAAACAGGATACCA
  AAGTTGAGCTGGAGACTTACAAGCAAACTCGGCAAGGTCTGGATGAAATGTACAGTGATG
  TGTGGAAGCAGCTAAAAGAGGAGAAGAAAGTCCGGTTGGAACTGGAAAAAGAACTGGAGT
  TACAAATTGGAATGAAAACCGAAATGGAAATTGCAATGAAGTTACTGGAAAAGGACACCC
  ACGAGAAGCAGGACACACTAGTTGCCCTCCGCCAGCAGCTGGAAGAAGTCAAAGCGATTA
  ATTTACAGATGTTTCACAAAGCTCAGAATGCAGAGAGCAGTTTGCAGCAGAAGAATGAAG
  CCATCACATCCTTTGAAGGAAAAACCAACCAAGTTATGTCCAGCATGAAACAAATGGAAG
  AAAGGTTGCAGCACTCGGAGCGGGCGAGGCAGGGAGCTGAGGAGCGGAGCCACAAGCTGC
  AGCAGGAGCTGGGCGGGAGGATCGGCGCCCTGCAGCTGCAGCTCTCCCAGCTGCACGAGC
  AATGCTCAAGCCTGGAGAAAGAATTGAAATCAGAAAAAGAGCAAAGACAGGCTCTTCAGC
  GCGAATTACAGCACGAGAAAGACACTTCCTCTCTACTCAGGATGGAGCTGCAACAAGTGG
  AAGGACTGAAAAAGGAGTTGCGGGAGCTTCAGGACGAGAAGGCAGAGCTGCAGAAGATCT
  GCGAGGAGCAGGAACAAGCCCTCCAGGAAATGGGCCTGCACCTCAGCCAGTCCAAGCTGA
  AGATGGAAGATATAAAAGAAGTGAACCAGGCACTGAAGGGCCACGCCTGGCTGAAAGATG
  ACGAAGCGACACACTGTAGGCAGTGTGAGAAGGAGTTCTCCATTTCCCGGAGAAAGCACC
  ACTGCCGGAACTGTGGCCACATCTTCTGCAACACCTGCTCCAGCAACGAGCTGGCCCTGC
  CCTCCTACCCCAAGCCGGTGCGAGTGTGCGACAGCTGCCACACCCTGCTCCTGCAGCGCT
  GCTCCTCCACGGCCTCCTGAACGTCCGTCCTCAGGAGCACAGCCTCACGGACAGTGCCAA
  ACCCTGTGGGTCTCCAGGGGCTTGGGAAATGTGTTCTTTCCCAAGAGTATCAAAGGAAAG
  AATCAAATTTCTTGCCCGGTCACTGGCACTCCAGAAGACAGCGTGCCGGAACCGGCAGCT
  CTCACCTTTCTGTGACTTGTTCGGAATTAACTCCTCTGGATGGAAACTTCCATCTTACTT
  GGTTACATCACGGCTCTGGTTCAGATACAACTTCATGATTTTGCTACTATCATTTTTCAC
  TTTTCAAAGAATTTAACCTATTTTACAGCAGTTCAGTTCTGCTAGTGAGTAGTTTTCCTC
  TCCTACCTTCCTTCTAAAAACCTGATTCATGCACAGCGTTTGACACACATGGAGTCTGCC
  AGTGTGCCTTCTCTGCTTCAGACAAGAGATCTGCCATTTCATGCCCTTGTGACTACCTAT
  CATTGGCCCTGCAATAAAATCATTTATTTTTCAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001040451.1 unedited
  TGCTAGCCTGGAAGCCTCCCGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAG
  CCTCCGGACTCTAGCCTAGGCCGCGGGACGGATAACAATTTCACACAGGAAACAGCTATG
  ACCATTAGGCCTATTTAGGTGACACTATAGAACAAGTTTGTACAAAAAAGCAGGCTGGTA
  CCGGTCCGGAATTCCCGGGATCTGGGAGAGGCCTCTGGAGCAGGAGGCCCAGTGGCTCTT
  CTGACCCAAGGCCCCGCCGTCCAGCTTCTAAGTGCCAGATGATGGAGGAGCGTGCCAACC
  TGATGCACATGATGAAACTCAGCATCAAGGTGTTGCTCCAGTCGGCTCTGAGCCTGGGCC
  GCAGCCTGGATGCGGACCATGCCCCCTTGCAGCAGTTCTTTGTAGTGATGGAGCACTGCC
  TCAAACATGGGCTGAAAGTTAAGAAGAGTTTTATTGGCCAAAATAAATCATTCTTTGGTC
  CTTTGGAGCTGGTGGAGAAACTTTGTCCAGAAGCATCAGATATAGCGACTAGTGTCAGAA
  ATCTTCCAGAATTAAAGACAGCTGTGGGAAGAGGCCGAGCGTGGCTTTATCTTGCACTCA
  TGCAAAAGAAACTGGCAGATTATCTGAAAGTGCTTATAGACAATAAACATCTCTTAAGCG
  AGTTCTATGAGCCTGAGGCTTTAATGATGGAGGAAGAAGGGATGGTGATTGTTGGTCTGC
  TGGTGGGACTCAATGTTCTCGATGCCAATCTCTGCTTGAAAGGAGAAGACTTGGATTCTC
  AGGTTGGAGTAATAGATTTTTCCCTCTACCTTAAGATGTGCAGATCTGATGGTGGCAAGG
  AGCATGAAAGATTACTGATGTCTGATCAAAAAAATTATGTGGAGACTTACGCACTGACTG
  CCAGTGGGATCTCAACCAGATAGATGGCTGGAAAAGACTACCTCAAGCCTTCAGGGAGAG
• Restriction Sites: Please inquire
• ACCN: NM_001040451
• ORF Size: 1803 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001040451.1, NP_001035541.1
• RefSeq Size: 2628
• RefSeq ORF: 1803
• Locus ID: 80230
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (b) which has a shorter N-terminus compared to isoform a.