Endothelin 3 (EDN3) (NM_207034) Human Untagged Clone

CAT#: SC324408

EDN3 (untagged)-Human endothelin 3 (EDN3), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EDN3
• Synonyms: ET-3; ET3; HSCR4; PPET3; WS4B
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_207034.1 CGCTGTCCTGGGTGCGCCTCGGGCCGCTGCGCTCCTGGCCAGCTCCCCCCAGCGCCTGGG
  AGGCGACCCCCAGTGCGCCCGGGTTCAAAGGCCCGGCGAGGCAGTTCCAGCCCCTCTGGG
  GGGCGGGGAGGCGACGGGGGTGGTGCAGAAGCCAGAAAAGCCCGAGCCACAGCCGGCCAG
  CTCCGCGCAGGGATGGGCAGCGCGCTCTGAAAGTTTATGACCGCCGCAGCCAACTCCTGG
  CCGGAGCTGGAGACGCAGCGAGCGATCGGCCGGCCTCGAACCCCCACAGCTGGAGGGCGA
  GGCCAGCTGTACCCGGCCCCAGTGCCCTTTCGCGGCCACAAGCGGCCGTCCTCCTGGTCC
  GGTGCTCCGGCGCCTGATCTAGGTTCATGGAGCCGGGGCTGTGGCTCCTTTTCGGGCTCA
  CAGTGACCTCCGCCGCAGGATTCGTGCCTTGCTCCCAGTCTGGGGATGCTGGCAGGCGCG
  GCGTGTCCCAGGCCCCCACTGCAGCCAGATCTGAGGGGGACTGTGAAGAGACTGTGGCTG
  GCCCTGGCGAGGAGACTGTGGCTGGCCCTGGCGAGGGGACTGTGGCCCCGACAGCACTGC
  AGGGTCCAAGCCCTGGAAGCCCTGGGCAGGAGCAGGCGGCCGAGGGGGCCCCTGAGCACC
  ACCGATCCAGGCGCTGCACGTGCTTCACCTACAAGGACAAGGAGTGTGTCTACTATTGCC
  ACCTGGACATCATTTGGATCAACACTCCCGAACAGACGGTGCCCTATGGACTGTCCAACT
  ACAGAGGAAGCTTCCGGGGCAAGAGGTCTGCGGGGCCACTTCCAGGGAATCTGCAGCTCT
  CACATCGGCCACACTTGCGCTGCGCTTGTGTGGGGAGATATGACAAGGCCTGCCTGCACT
  TTTGCACCCAAACTCTGGACGTCAGCAGTAATTCAAGGACGGCAGAAAAAACAGACAAAG
  AAGAGGAAGGGAAGGTTGAAGTCAAGGACCAACAAAGCAAGCAGGCTTTAGACCTCCACC
  ATCCAAAGCTCATGCCCGGCAGTGGACTCGCCCTCGCTCCATCTACCTGCCCCCGCTGCC
  TCTTTCAGGAAGGAGCCCCTTAGGAGGACAGGCCTGCAGCATCCTGGTCTCGGGAGGCTT
  CTGTCATTGCTCACACACAGTTCAGATTTCCACCTCTTTATAGACAAGAAGTGAATTTGC
  CTGGGGCAGAACACCCACCCAAAGAGTCCCCACTTAACAATACCCCCCCCCCACGGCAAG
  AATGCCCAAATCCGAATGACCCCAGTTTTCCTAATGAGTAAAATGATCCCAGATGTGCCC
  CAGAGCATGACGCCTGCAGCTCCGGTTTCATGCAGGAAATTGGTTTTGGAGAGTTTTGGC
  AAGTTGGAAAGCCACTTACTGGCTTTTGACATGACTTCTCTTGGAGAATAAGTGGACTCC
  AAGCTAACTCTTTGCAAATGTAAACACATGTCCATCTTGTAATAAATGCAAAATGCCCGT
  GCAGCAGAAGCATGCGACTTTCATATCCTTGCCTAGAATAGGCTGCATGGTGTATGTCAG
  TGAGGGCCACGAGGCGTCGGCTTTAGACACAGATCATAGCTCTACAGGAGTTTATGAATT
  TGAAGCTTATGGGATTTTGGCAGAGAAATTTTCAGCTGTGCTTGATACCCACCAAAAGAA
  TGTATCTCGAAAGAATGAAGGAAGAAGAAAAAAGGATCCTTGATGTTTGTGACAAGAAAA
  TGAGAAAGTTAGTATCTGCAATACAGAGCTTGTTCCTGTTCAGTGACTGACCCTCTGTAT
  TCTGTATAGACACCAGGCCGATACACAGTGGAGTTCCCAGGCCTTGTTTGCAGGAAGCCG
  ACTGTAAAGACAGCCCCAGCTCAAGGCTATTAGGTTGAATATTTGCTTTCATGAGTAAAT
  GTGGATCTTTGGGGAATGGCTTCAAAATAAGTCACGAACACAAATTCTTTGTAAATTATG
  TAAATTCCTGTTTATATAAATTGGCAACAACTTATACCGTCTGACAGTTCAAAATCTCTT
  TCAGCTGCGCTCTTCCCACCGAGCCGAGCTTACTGTGAGTGTGGAGATGTTATCCCACCA
  TGTAAAGTCGCCTGCGCAGGGGAGGGCTGCCCATCTCCCCAACCCAGTCACAGAGAGATA
  GGAAACGGCATTTGAGTGGGTGTCCAGGGCCCCGTAGAGAGACATTTAAGATGGTGTATG
  ACAGAGCATTGGCCTTGACCAAATGTTAAATCCTCTGTGTGTATTTCATAAGTTATTACA
  GGTATAAAAGTGATGACCTATCATGAGGAAATGAAAGTGGCTGATTTGCTGGTAGGATTT
  TGTACAGTTTAGAGAAGCGATTATTTATTGTGAAACTGTTCTCCACTCCAACTCCTTTAT
  GTGGATCTGTTCAAAGTAGTCACTGTATATACGTATAGAGAGGTAGATAGGTAGGTAGAT
  TTTAAATTGCATTCTGAATACAAACTCATACTCCTTAGAGCTTGAATTACATTTTTAAAA
  TGCATATGTGCTGTTTGGCACCGTGGCAAGATGGTATCAGAGAGAAACCCATCAATTGCT
  CAAATACTCAGAAAGTACTGTCAAAAGCCTAATAAAAAACCTAAAGTTTGCTCTGAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_207034
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207034.1, NP_996917.1
• RefSeq Size: 2659 bp
• RefSeq ORF: 717 bp
• Locus ID: 1908
• Cytogenetics: 20q13.32
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]'
  Transcript Variant: This variant (4) encodes the longest isoform (1).