MTHFD2 (NM_001040409) Human Untagged Clone

CAT#: SC324565

MTHFD2 (untagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MTHFD2
• Synonyms: methylenetetrahydrofolate dehydro; methylene tetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase; methylene tetrahydrofolate dehydrogenase 2; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase; NMDMC
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001040409.1 GGGGCCCGGCGCAGTCACCGGCGCGGTCTATGGCTGCGACTTCTCTAATGTCTGCTTTGG
  CTGCCCGGCTGCTGCAGCCCGCGCACAGCTGCTCCCTTCGCCTTCGCCCTTTCCACCTCG
  CGGCAGTTCGGGGAATCTCTCCCTAGGTCAGGTTGGAGTGCAGTGCTGCAATCACGGCTT
  ACTGCAGCCTTGACCTCCTGGGCTCAAGTGATCCTCCCACCTCAGCTTAAATGAAGCTGT
  TGTCATTTCTGGAAGGAAACTGGCCCAGCAGATCAAGCAGGAAGTGCGGCAGGAGGTAGA
  AGAGTGGGTGGCCTCAGGCAACAAACGGCCACACCTGAGTGTGATCCTGGTTGGCGAGAA
  TCCTGCAAGTCACTCCTATGTCCTCAACAAAACCAGGGCAGCTGCAGTTGTGGGAATCAA
  CAGTGAGACAATTATGAAACCAGCTTCAATTTCAGAGGAAGAATTGTTGAATTTAATCAA
  TAAACTGAATAATGATGATAATGTAGATGGCCTCCTTGTTCAGTTGCCTCTTCCAGAGCA
  TATTGATGAGAGAAGGATCTGCAATGCTGTTTCTCCAGACAAGGATGTTGATGGCTTTCA
  TGTAATTAATGTAGGACGAATGTGTTTGGATCAGTATTCCATGTTACCGGCTACTCCATG
  GGGTGTGTGGGAAATAATCAAGCGAACTGGCATTCCAACCCTAGGGAAGAATGTGGTTGT
  GGCTGGAAGGTCAAAAAACGTTGGAATGCCCATTGCAATGTTACTGCACACAGATGGGGC
  GCATGAACGTCCCGGAGGTGATGCCACTGTTACAATATCTCATCGATATACTCCCAAAGA
  GCAGTTGAAGAAACATACAATTCTTGCAGATATTGTAATATCTGCTGCAGGTATTCCAAA
  TCTGATCACAGCAGATATGATCAAGGAAGGAGCAGCAGTCATTGATGTGGGAATAAATAG
  AGTTCACGATCCTGTAACTGCCAAACCCAAGTTGGTTGGAGATGTGGATTTTGAAGGAGT
  CAGACAAAAAGCTGGGTATATCACTCCAGTTCCTGGAGGTGTTGGCCCCATGACAGTGGC
  AATGCTAATGAAGAATACCATTATTGCTGCAAAAAAGGTGCTGAGGCTTGAAGAGCGAGA
  AGTGCTGAAGTCTAAAGAGCTTGGGGTAGCCACTAATTAACTACTGTGTCTTCTGTGTCA
  CAAACAGCACTCCAGGCCAGCTCAAGAAGCAAAGCAGGCCAATAGAAATGCAATATTTTT
  AATTTATTCTACTGAAATGGTTTAAAATGATGCCTTGTATTTATTGAAAGCTTAAATGGG
  TGGGTGTTTCTGCACATACCTCTGCAGTACCTCACCAGGGAGCATTCCAGTATCATGCAG
  GGTCCTGTGATCTAGCCAGGAGCAGCCATTAACCTAGTGATTAATATGGGAGACATTACC
  ATATGGAGGATGGATGCTTCACTTTGTCAAGCACCTCAGTTACACATTCGCCTTTTCTAG
  GATTGCATTTCCCAAGTGCTATTGCAATAACAGTTGATACTCATTTTAGGTACCAAACCT
  TTTGAGTTCAACTGACCAAACCAAAGGAAAAGTGTTGCTAGAGAAAATTAGGGAAAAGGT
  GAAAAAGAAAAAATGGTAGTAATTGAGCAGAAAAAAATTAATTTATATATGTATTGATTG
  GCAACCAGATTTATCTAAGTAGAACTGAATTGGCTAGGAAAAAAGAAAAACTGCATGTTA
  ATCATTTTCCTAAGCTGTCCTTTTGAGGCTTAGTCAGTTTATTGGGAAAATGTTTAGGAT
  TATTCCTTGCTATTAGTACTCATTTTATGTATGTTACCCTTCAGTAAGTTCTCCCCATTT
  TAGTTTTCTAGGACTGAAAGGATTCTTTTCTACATTATACATGTGTGTTGTCATATTTGG
  CTTTTGCTATATACTTTAACTTCATTGTTAAATTTTTGTATTGTATAGTTTCTTTGGTGT
  ATCTTAAAACCTATTTTTGAAAAACAAACTTGGCTTGATAATCATTTGGGCAGCTTGGGT
  AAGTACGCAACTTACTTTTCCACCAAAGAACTGTCAGCAGCTGCCTGCTTTTCTGTGATG
  TATGTATCCTGTTGACTTTTCCAGAAATTTTTTAAGAGTTTGAGTTACTATTGAATTTAA
  TCAGACTTTCTGATTAAAGGGTTTTCTTTCTTTTTTAATAAAACACATCTGTCTGGTATG
  GTACCAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001040409.1 unedited
  GTCGTACCATTTGTATACGACTCCTATAGGGCGGCCGGGAATCGGCCATTACGGCCGGGG
  CCCGGCGCAGTCACCGGCGCGGTCTATGGCTGCGACTTCTCTAATGTCTGCTTTGGCTGC
  CCGGCTGCTGCAGCCCGCGCACAGCTGCTCCCTTCGCCTTCGCCCTTTCCACCTCGCGGC
  AGTTCGGGGAATCTCTCCCTAGGTCAGGTTGGAGTGCAGTGCTGCAATCACGGCTTACTG
  CAGCCTTGACCTCCTGGGCTCAAGTGATCCTCCCACCTCAGCTTAAATGAAGCTGTTGTC
  ATTTCTGGAAGGAAACTGGCCCAGCAGATCAAGCAGGAAGTGCGGCAGGAGGTAGAAGAG
  TGGGTGGCCTCAGGCAACAAACGGCCACACCTGAGTGTGATCCTGGTTGGCGAGAATCCT
  GCAAGTCACTCCTATGTCCTCAACAAAACCAGGGCAGCTGCAGTTGTGGGAATCAACAGT
  GAGACAATTATGAAACCAGCTTCAATTTCAGAGGAAGAATTGTTGAATTTAATCAATAAA
  CTGAATAATGATGATAATGTAGATGGCCTCCTTGTTCAGTTGCCTCTTCCAGAGCATATT
  GATGAGAGAAGGATCTGCAATGCTGTTTCTCCAGACAAGGATGTTGATGGCTTTCATGTA
  ATTAATGTAGGACGAATGTGTTTGGATCAGTATTCCATGTTACCGGCTACTCCATGGGGT
  GTGTGGGAAATAATCAAGCGAACTGGCATTCCAACCCTAGGGAAGATGTGGTTGTGGCTG
  GAGGTCAAAAAACGTGGATTGCCCATTGCAATGTACTGCACCACAGATGGGGCGCATGAA
  CGTTCCCGGAGGTGATGCACCTGTAACAATATCCTCATCGATTTACTTCCAAGGAGCAGT
  GAGAACAATACATTCTTTGGCGAGATATATTGTAATATTCTTGCGCGTCGCAGGTATAAT
  CCAAATATTCTGGAGTACTCA
• Restriction Sites: Please inquire
• ACCN: NM_001040409
• ORF Size: 747 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001040409.1, NP_001035499.1
• RefSeq Size: 2306
• RefSeq ORF: 747
• Locus ID: 10797
• Protein Families: Druggable Genome
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate
• Gene Summary: This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (2) contains an alternate exon in the 5' coding region and uses a downstream start codon, compared to variant 1. The encoded protein (isoform B) has a shorter N-terminus which lacks a mitochondrial transit peptide and a tetrahydrofolate dehydrogenase/cyclohydrolase catalytic domain, compared to isoform A.