XRCC4 (NM_022406) Human Untagged Clone

CAT#: SC324607

XRCC4 (untagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 4 (XRCC4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: XRCC4
• Synonyms: SSMED
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_022406.1 GGGGACCGGAAGTAGAGTCACGGAGAGGTAGGATCCGGAAGTGGGGCTGCCTCTTTAAAT
  AACAAAAATCTGAGGTATTAAGAAATGGAGAGAAAAATAAGCAGAATCCACCTTGTTTCT
  GAACCCAGTATAACTCATTTTCTACAAGTATCTTGGGAGAAAACACTGGAATCTGGTTTT
  GTTATTACACTTACTGATGGTCATTCAGCATGGACTGGGACAGTTTCTGAATCAGAGATT
  TCCCAAGAAGCTGATGACATGGCAATGGAAAAAGGGAAATATGTTGGTGAACTGAGAAAA
  GCATTGTTGTCAGGAGCAGGACCAGCTGATGTATACACGTTTAATTTTTCTAAAGAGTCT
  TGTTATTTCTTCTTTGAGAAAAACCTGAAAGATGTCTCATTCAGACTTGGTTCCTTCAAC
  CTAGAGAAAGTTGAAAACCCAGCTGAAGTCATTAGAGAACTTATTTGTTATTGCTTGGAC
  ACCATTGCAGAAAATCAAGCCAAAAATGAGCACCTGCAGAAAGAAAATGAAAGGCTTCTG
  AGAGATTGGAATGATGTTCAAGGACGATTTGAAAAATGTGTGAGTGCTAAGGAAGCTTTG
  GAGACTGATCTTTATAAGCGGTTTATTCTGGTGTTGAATGAGAAGAAAACAAAAATCAGA
  AGTTTGCATAATAAATTATTAAATGCAGCTCAAGAACGAGAAAAGGACATCAAACAAGAA
  GGGGAAACTGCAATCTGTTCTGAAATGACTGCTGACCGAGATCCAGTCTATGATGAGAGT
  ACTGATGAGGAAAGTGAAAACCAAACTGATCTCTCTGGGTTGGCTTCAGCTGCTGTAAGT
  AAAGATGATTCCATTATTTCAAGTCTTGATGTCACTGATATTGCACCAAGTAGAAAAAGG
  AGACAGCGAATGCAAAGAAATCTTGGGACAGAACCTAAAATGGCTCCTCAGGAGAATCAG
  CTTCAAGAAAAGGAAAATTCTAGGCCTGATTCTTCACTACCTGAGACGTCTAAAAAGGAG
  CACATCTCAGCTGAAAACATGTCTTTAGAAACTCTGAGAAACAGCAGCCCAGAAGACCTC
  TTTGATGAGATTTAACAGTCTCAAAAAATACTTTGATGTTCACTAGACTATGTTTTCTAT
  TCATTTCTTTAAAATGAAAAAGGAGAATTTCAAGTCAGCAGCCGCTATTACCGTATCTTA
  CAATTTAATTACATACACAGTGAATTGAAACCATTGTGCAAAATGGATTACACATGTATA
  CAAAGATACGATTTGATGATGACACTGGCACATTATTCTAAACTATTCATTCAGCATGCC
  TATAATTACATAAATTGTATGAGACTTTTTGTTGCAAAGGACACATTTATCATATTCATT
  CACACATATTATATGTGATAGCTGTCCAACATCCTGTCTGGGAAGATTTTGAAAACAGGA
  CAAAGAAAACATCACTTTAAAATGTCTTCAGCTTTTTTTGAATAGACGTATTCAAACATA
  TTCTGAACATTGATGTTTGAACATTTTAATTTGTGTGATGATGTAGAAAATATAATTTTA
  GTTTGTACATAAACATTGTGAAAATCTGATAATAAAATTTTTGATACACTAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_022406
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022406.1, NP_071801.1
• RefSeq Size: 1665 bp
• RefSeq ORF: 1011 bp
• Locus ID: 7518
• Cytogenetics: 5q14.2
• Protein Families: Druggable Genome
• Protein Pathways: Non-homologous end-joining
• Gene Summary: 'The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternate transcript variants such as NM_022406 are unlikely to be expressed in some individuals due to a polymorphism (rs1805377) in the last splice acceptor site. [provided by RefSeq, Oct 2019]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (2) is longer than isoform 1. Variants 2 and 4 encode the same isoform (2).