P4HA1 (NM_001142595) Human Untagged Clone

CAT#: SC325169

P4HA1 (untagged)-Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: P4HA1
• Synonyms: P4HA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001142595, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATCTGGTATATATTAATTATAGGAATTCTGCTTCCCCAGTCTTTGGCTCATCCAGGCTTTTTTACTT
  CAATTGGTCAGATGACTGATTTGATCCATACTGAGAAAGATCTGGTGACTTCTCTGAAAGATTATATTAA
  GGCAGAAGAGGACAAGTTAGAACAAATAAAAAAATGGGCAGAGAAGTTAGATCGGCTAACTAGTACAGCG
  ACAAAAGATCCAGAAGGATTTGTTGGGCATCCAGTAAATGCATTCAAATTAATGAAACGTCTGAATACTG
  AGTGGAGTGAGTTGGAGAATCTGGTCCTTAAGGATATGTCAGATGGCTTTATCTCTAACCTAACCATTCA
  GAGACAGTACTTTCCTAATGATGAAGATCAGGTTGGGGCAGCCAAAGCTCTGTTACGTCTCCAGGATACC
  TACAATTTGGATACAGATACCATCTCAAAGGGTAATCTTCCAGGAGTGAAACACAAATCTTTTCTAACGG
  CTGAGGACTGCTTTGAGTTGGGCAAAGTGGCCTATACAGAAGCAGATTATTACCATACGGAACTGTGGAT
  GGAACAAGCCCTAAGGCAACTGGATGAAGGCGAGATTTCTACCATAGATAAAGTCTCTGTTCTAGATTAT
  TTGAGCTATGCGGTATATCAGCAGGGAGACCTGGATAAGGCACTTTTGCTCACAAAGAAGCTTCTTGAAC
  TAGATCCTGAACATCAGAGAGCTAATGGTAACTTAAAATATTTTGAGTATATAATGGCTAAAGAAAAAGA
  TGTCAATAAGTCTGCTTCAGATGACCAATCTGATCAGAAAACTACACCAAAGAAAAAAGGGGTTGCTGTG
  GATTACCTGCCAGAGAGACAGAAGTACGAAATGCTGTGCCGTGGGGAGGGTATCAAAATGACCCCTCGGA
  GACAGAAAAAACTCTTTTGCCGCTACCATGATGGAAACCGTAATCCTAAATTTATTCTGGCTCCAGCTAA
  ACAGGAGGATGAATGGGACAAGCCTCGTATTATTCGCTTCCATGATATTATTTCTGATGCAGAAATTGAA
  ATCGTCAAAGACCTAGCAAAACCAAGGCTGAGGCGAGCCACCATTTCAAACCCAATAACAGGAGACTTGG
  AGACGGTACATTACAGAATTAGCAAAAGTGCCTGGCTCTCTGGCTATGAAAATCCTGTGGTGTCTCGAAT
  TAATATGAGAATACAAGATCTAACAGGACTAGATGTTTCCACAGCAGAGGAATTACAGGTAGCAAATTAT
  GGAGTTGGAGGACAGTATGAACCCCATTTTGACTTTGCACGGAAAGATGAGCCAGATGCTTTCAAAGAGC
  TGGGGACAGGAAATAGAATTGCTACATGGCTGTTTTATATGAGTGATGTGTCTGCAGGAGGAGCCACTGT
  TTTTCCTGAAGTTGGAGCTAGTGTTTGGCCCAAAAAAGGAACTGCTGTTTTCTGGTATAATCTGTTTGCC
  AGTGGAGAAGGAGATTATAGTACACGGCATGCAGCCTGTCCAGTGCTAGTTGGCAACAAATGGGTATCCA
  ATAAATGGCTCCATGAACGTGGACAAGAATTTCGAAGACCTTGTACGTTGTCAGAATTGGAATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142595
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142595.1, NP_001136067.1
• RefSeq Size: 2953 bp
• RefSeq ORF: 1605 bp
• Locus ID: 5033
• Cytogenetics: 10q22.1
• Protein Families: Druggable Genome, P450
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) differs in the 5' UTR and contains an alternate in-frame exon, compared to variant 1. It encodes isoform 2, which is the same length as isoform 1. Variants 2 and 3 both encode isoform 2.