bcl 6 (BCL6) (NM_001130845) Human Untagged Clone

CAT#: SC325294

BCL6 (untagged)-Human B-cell CLL/lymphoma 6 (BCL6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BCL6
• Synonyms: BCL5; BCL6A; LAZ3; ZBTB27; ZNF51
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001130845 edited
  TAAACTCCCACTCCCATGTGTCTTCAGCTTTCTTTTGCAAAGGAGAAAATCCTTGAAGTT
  TGGTAAAGACCGAGTTAGTCTATCTCTCTTTGCCTATCTCGAGTTGGGCTGGGGAGAGGA
  GGAGATAGGTTCTTTTGTCTTTTTCTGTCTTCTCCCTTCCCCACTTCCTTCCCTCCAGTC
  CCCACTCACTCACATGCACACACTAACCTTGGAGCCGATGGGATTGAGTGACTGGCACTT
  GGGACCACAGAGAAATGTCAGAGTGTTTGGTTACAGACTCAAGGAAACCTCTCATTTTAG
  AGTGCTCATTTGGTTTTGAGCAAAATTTTGGACTGTGAAGCAAGGCATTGGTGAAGACAA
  AATGGCCTCGCCGGCTGACAGCTGTATCCAGTTCACCCGCCATGCCAGTGATGTTCTTCT
  CAACCTTAATCGTCTCCGGAGTCGAGACATCTTGACTGATGTTGTCATTGTTGTGAGCCG
  TGAGCAGTTTAGAGCCCATAAAACGGTCCTCATGGCCTGCAGTGGCCTGTTCTATAGCAT
  CTTTACAGACCAGTTGAAATGCAACCTTAGTGTGATCAATCTAGATCCTGAGATCAACCC
  TGAGGGATTCTGCATCCTCCTGGACTTCATGTACACATCTCGGCTCAATTTGCGGGAGGG
  CAACATCATGGCTGTGATGGCCACGGCTATGTACCTGCAGATGGAGCATGTTGTGGACAC
  TTGCCGGAAGTTTATTAAGGCCAGTGAAGCAGAGATGGTTTCTGCCATCAAGCCTCCTCG
  TGAAGAGTTCCTCAACAGCCGGATGCTGATGCCCCAAGACATCATGGCCTATCGGGGTCG
  TGAGGTGGTGGAGAACAACCTGCCACTGAGGAGCGCCCCTGGGTGTGAGAGCAGAGCCTT
  TGCCCCCAGCCTGTACAGTGGCCTGTCCACACCGCCAGCCTCTTATTCCATGTACAGCCA
  CCTCCCTGTCAGCAGCCTCCTCTTCTCCGATGAGGAGTTTCGGGATGTCCGGATGCCTGT
  GGCCAACCCCTTCCCCAAGGAGCGGGCACTCCCATGTGATAGTGCCAGGCCAGTCCCTGG
  TGAGTACAGCCGGCCGACTTTGGAGGTGTCCCCCAATGTGTGCCACAGCAATATCTATTC
  ACCCAAGGAAACAATCCCAGAAGAGGCACGAAGTGATATGCACTACAGTGTGGCTGAGGG
  CCTCAAACCTGCTGCCCCCTCAGCCCGAAATGCCCCCTACTTCCCTTGTGACAAGGCCAG
  CAAAGAAGAAGAGAGACCCTCCTCGGAAGATGAGATTGCCCTGCATTTCGAGCCCCCCAA
  TGCACCCCTGAACCGGAAGGGTCTGGTTAGTCCACAGAGCCCCCAGAAATCTGACTGCCA
  GCCCAACTCGCCCACAGAGTCCTGCAGCAGTAAGAATGCCTGCATCCTCCAGGCTTCTGG
  CTCCCCTCCAGCCAAGAGCCCCACTGACCCCAAAGCCTGCAACTGGAAGAAATACAAGTT
  CATCGTGCTCAACAGCCTCAATCAGAATGCCAAACCAGAGGGGCCTGAGCAGGCTGAGCT
  GGGCCGCCTTTCCCCACGAGCCTACACGGCCCCACCTGCCTGCCAGCCACCCATGGAGCC
  TGAGAACCTTGACCTCCAGTCCCCAACCAAGCTGAGTGCCAGCGGGGAGGACTCCACCAT
  CCCACAAGCCAGCCGGCTCAATAACATCGTTAACAGGTCCATGACGGGCTCTCCCCGCAG
  CAGCAGCGAGAGCCACTCACCACTCTACATGCACCCCCCGAAGTGCACGTCCTGCGGCTC
  TCAGTCCCCACAGCATGCAGAGATGTGCCTCCACACCGCTGGCCCCACGTTCCCTGAGGA
  GATGGGAGAGACCCAGTCTGAGTACTCAGATTCTAGCTGTGAGAACGGGGCCTTCTTCTG
  CAATGAGTGTGACTGCCGCTTCTCTGAGGAGGCCTCACTCAAGAGGCACACGCTGCAGAC
  CCACAGTGACAAACCCTACAAGTGTGACCGCTGCCAGGCCTCCTTCCGCTACAAGGGCAA
  CCTCGCCAGCCACAAGACCGTCCATACCGGTGAGAAACCCTATCGTTGCAACATCTGTGG
  GGCCCAGTTCAACCGGCCAGCCAACCTGAAAACCCACACTCGAATTCACTCTGGAGAGAA
  GCCCTACAAATGCGAAACCTGCGGAGCCAGATTTGTACAGGTGGCCCACCTCCGTGCCCA
  TGTGCTTATCCACACTGGTGAGAAGCCCTATCCCTGTGAAATCTGTGGCACCCGTTTCCG
  GCACCTTCAGACTCTGAAGAGCCACCTGCGAATCCACACAGGAGAGAAACCTTACCATTG
  TGAGAAGTGTAACCTGCATTTCCGTCACAAAAGCCAGCTGCGACTTCACTTGCGCCAGAA
  GCATGGCGCCATCACCAACACCAAGGTGCAATACCGCGTGTCAGCCACTGACCTGCCTCC
  GGAGCTCCCCAAAGCCTGCTGAAGCATGGAGTGTTGATGCTTTCGTCTCCAGCCCCTTCT
  CAGAATCTACCCAAAGGATACTGTAACACTTTACAATGTTCATCCCATGATGTAGTGCCT
  CTTTCATCCACTAGTGCAAATCATAGCTGGGGGTTGGGGGTGGTGGGGGTCGGGGCCTGG
  GGGACTGGGAGCCGCAGCAGCTCCCCCTCCCCCACTGCCATAAAACATTAAGAAAATCAT
  ATTGCTTCTTCTCCTATGTGTAAGGTGAACCATGTCAGCAAAAAGCAAAATCATTTTATA
  TGTCAAAGCAGGGGAGTATGCAAAAGTTCTGACTTGACTTTAGTCTGCAAAATGAGGAAT
  GTATATGTTTTGTGGGAACAGATGTTTCTTTTGTATGTAAATGTGCATTCTTTTAAAAGA
  CAAGACTTCAGTATGTTGTCAAAGAGAGGGCTTTAATTTTTTTAACCAAAGGTGAAGGAA
  TATATGGCAGAGTTGTAAATATATAAATATATATATATATATAAAATAAATATATATAAA
  CCTAAAAAAGATATATTAAAAATATAAAACTGCGTTAAAGGCTCGATTTTGTATCTGCAG
  GCAGACACGGATCTGAGAATCTTTATTGAGAAAGAGCACTTAAGAGAATATTTTAAGTAT
  TGCATCTGTATAAGTAAGAAAATATTTTGTCTAAAATGCCTCAGTGTATTTGTATTTTTT
  TGCAAGTGAAGGTTTACAATTTACAAAGTGTGTATTAAAAAAAACAAAAAGAACAAAAAA
  ATCTGCAGAAGGAAAAATGTGTAATTTTGTTCTAGTTTTCAGTTTGTATATACCCGTACA
  ACGTGTCCTCACGGTGCCTTTTTTCACGGAAGTTTTCAATGATGGGCGAGCGTGCACCAT
  CCCTTTTTGAAGTGTAGGCAGACACAGGGACTTGAAGTTGTTACTAACTAAACTCTCTTT
  GGGAATGTTTGTCTCATCCCATTCTGCGTCATGCTTGTGTTATAACTACTCCGGAGACAG
  GGTTTGGCTGTGTCTAAACTGCATTACCGCGTTGTAAAATATAGCTGTACAAATATAAGA
  ATAAAATGTTGAAAAGTCAAAAAAAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001130845
• Insert Size: 3600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001130845.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130845.1, NP_001124317.1
• RefSeq Size: 3662 bp
• RefSeq ORF: 2121 bp
• Locus ID: 604
• Cytogenetics: 3q27.3
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Aug 2015]'
  Transcript Variant: This variant (2) has a downstream and alternate 5' UTR exon, as compared to variant 1.