beta COP (COPB1) (NM_001144062) Human Untagged Clone

CAT#: SC325378

COPB1 (untagged)-Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COPB1
• Synonyms: COPB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001144062, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACGGCGGCTGAGAACGTATGCTACACGTTAATTAACGTGCCAATGGATTCAGAACCACCATCTGAAA
  TTAGCTTAAAAAATGATCTAGAAAAAGGAGATGTAAAGTCAAAGACTGAAGCTTTGAAGAAAGTAATCAT
  TATGATTCTGAATGGTGAAAAACTTCCTGGACTTCTGATGACCATCATTCGTTTTGTGCTACCTCTTCAG
  GATCACACTATCAAGAAATTACTTCTGGTATTTTGGGAAATTGTTCCTAAAACAACTCCAGATGGGAGAC
  TTTTACATGAGATGATCCTTGTATGTGATGCATACAGAAAGGATCTTCAACATCCTAATGAATTTATTCG
  AGGATCTACTCTTCGTTTTCTTTGCAAATTGAAAGAAGCAGAATTGCTAGAACCTTTAATGCCAGCTATT
  CGTGCATGTTTGGAGCATCGACACAGCTATGTTAGAAGAAATGCTGTTTTGGCCATCTATACCATCTATA
  GAAATTTTGAACATCTTATACCTGATGCTCCTGAACTGATACATGATTTTCTGGTGAATGAGAAGGATGC
  AAGTTGCAAAAGGAATGCATTTATGATGCTAATTCATGCAGATCAGGATCGAGCTTTGGATTACTTAAGT
  ACTTGCATTGATCAAGTTCAAACATTTGGAGACATTCTGCAGCTGGTTATTGTTGAACTGATTTATAAGG
  TCTGTCATGCTAATCCATCAGAAAGAGCTCGTTTTATTCGCTGCATCTATAACTTATTACAGTCATCCAG
  CCCTGCTGTAAAATATGAAGCTGCTGGGACATTAGTGACACTCTCTAGTGCACCAACTGCAATCAAGGCT
  GCTGCTCAGTGTTACATTGATTTAATTATTAAGGAGAGCGACAACAATGTAAAACTCATAGTTTTGGATC
  GCTTGATAGAATTAAAAGAGCATCCTGCTCATGAACGAGTACTACAGGATCTGGTTATGGATATCCTAAG
  AGTATTGAGCACACCAGACTTAGAAGTACGAAAGAAAACTCTGCAGTTAGCACTGGATCTTGTCTCTTCT
  AGAAATGTTGAAGAGCTGGTTATTGTCCTGAAGAAGGAAGTGATAAAAACAAATAATGTGTCTGAGCATG
  AAGATACTGACAAATACAGACAACTCCTAGTGCGAACATTGCATTCCTGTTCTGTCCGATTTCCAGATAT
  GGCTGCAAATGTTATTCCTGTGTTAATGGAATTTCTCAGTGACAACAACGAAGCAGCAGCTGCTGATGTC
  TTGGAGTTTGTTCGTGAAGCCATTCAGCGCTTTGATAACCTGAGAATGCTTATTGTTGAGAAGATGCTTG
  AAGTCTTTCATGCTATTAAATCTGTCAAGATTTACCGAGGAGCATTATGGATCCTGGGAGAATACTGTAG
  TACCAAGGAAGACATTCAGAGTGTGATGACTGAGATCCGCAGGTCCCTTGGAGAGATCCCAATTGTAGAG
  TCAGAAATAAAGAAAGAAGCTGGTGAATTAAAACCTGAAGAAGAAATAACTGTAGGGCCAGTTCAGAAAT
  TGGTTACTGAAATGGGTACCTATGCAACTCAGAGTGCCCTTAGCAGTTCTAGACCCACCAAGAAAGAGGA
  AGACAGACCTCCCTTGAGAGGATTCCTTCTGGATGGAGATTTCTTTGTTGCTGCCTCCCTTGCCACAACT
  CTGACCAAGATTGCATTGCGCTATGTAGCTTTGGTTCAGGAGAAGAAAAAGCAAAATTCTTTTGTTGCTG
  AGGCTATGTTGCTCATGGCTACTATCCTGCATTTGGGAAAATCCTCTCTTCCTAAGAAGCCAATTACTGA
  TGATGATGTGGATCGAATTTCCCTGTGCCTCAAGGTCTTGTCTGAATGTTCACCTTTAATGAATGACATT
  TTCAATAAGGAATGCAGACAGTCCCTTTCTCACATGTTATCTGCTAAACTAGAAGAAGAGAAATTATCCC
  AAAAGAAAGAATCTGAAAAGAGGAATGTGACAGTACAGCCTGATGACCCCATTTCCTTCATGCAACTAAC
  TGCTAAGAATGAAATGAACTGCAAGGAAGATCAGTTTCAGCTGAGTTTACTGGCAGCAATGGGTAACACA
  CAGAGGAAAGAGGCAGCAGATCCCCTAGCATCTAAACTTAACAAGGTCACCCAATTGACAGGTTTCTCAG
  ATCCTGTATATGCAGAAGCTTACGTTCATGTCAACCAATATGATATTGTCCTGGATGTACTTGTTGTGAA
  CCAAACCAGTGATACTTTGCAGAATTGCACATTAGAACTAGCTACACTAGGGGATCTGAAACTTGTGGAA
  AAGCCGTCTCCTTTGACTCTTGCTCCTCATGACTTCGCAAATATTAAAGCTAACGTCAAAGTAGCATCAA
  CAGAAAATGGAATAATTTTTGGTAATATAGTTTATGATGTCTCTGGAGCAGCAAGTGACAGAAATTGTGT
  GGTTCTCAGTGATATTCACATCGACATCATGGACTATATCCAGCCTGCAACTTGCACTGATGCAGAATTC
  CGTCAGATGTGGGCCGAATTTGAATGGGAAAACAAAGTGACAGTTAACACCAACATGGTTGATTTAAATG
  ACTACTTACAGCACATATTAAAGTCAACCAATATGAAATGCCTGACTCCAGAAAAGGCCCTTTCTGGTTA
  CTGTGGCTTTATGGCAGCCAACCTTTATGCTCGTTCCATATTTGGTGAAGATGCACTTGCAAATGTCAGC
  ATTGAGAAGCCAATTCACCAGGGACCAGATGCTGCTGTTACCGGCCATATAAGAATTCGTGCAAAGAGCC
  AGGGAATGGCCTTAAGTCTTGGAGATAAAATCAACTTGTCACAGAAGAAAACTAGTATATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001144062
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001144062.1, NP_001137534.1
• RefSeq Size: 3368 bp
• RefSeq ORF: 2862 bp
• Locus ID: 1315
• Cytogenetics: 11p15.2
• Gene Summary: 'This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]'
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.