RPL28 (NM_001136135) Human Untagged Clone

CAT#: SC325550

RPL28 (untagged)-Human ribosomal protein L28 (RPL28), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL28
• Synonyms: L28
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001136135, the custom clone sequence may differ by one or more nucleotides
  ATGTCTGCGCATCTGCAATGGATGGTCGTGCGGAACTGCTCCAGTTTCCTGATCAAGAGG
  AATAAGCAGACCTACAGCACTGAGCCCAATAACTTGAAGGCCCGCAATTCCTTCCGCTAC
  AACGGACTGATTCACCGCAAGACTGTGGGCGTGGAGCCGGCAGCCGACGGCAAAGGTGTC
  GTGGTGGTCATTAAGCGGAGATCCGGCCAGCGGAAGCCTGCCACCTCCTATGTGCGGACC
  ACCATCAACAAGAATGCTCGCGCCACGCTCAGCAGCATCAGACACATGATCCGCAAGAAC
  AAGTACCGCCCCGACCTGCGCATGGACATGCTGGCATCTACTGGGTCAGGGCTCTGCTGC
  TCGGTGGCTGTGCAACCTTGGGCAAGTTCCTCAACCTCTCTGTGTCTTCGTACCCTCATC
  TGTAACATGCGTGTCGATAGACCCTACTACTCAGGGTTGATGAGAAGATTAAATGTGCAA
  AACCTGCTTGACTGTGCCCACAAATCC
  
• Restriction Sites: Please inquire
• ACCN: NM_001136135
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136135.1, NP_001129607.1
• RefSeq Size: 934 bp
• RefSeq ORF: 510 bp
• Locus ID: 6158
• Cytogenetics: 19q13.42
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]'
  Transcript Variant: This variant (3) lacks a stop-codon-containing segment in the 3' coding region, compared to variant 1. The encoded protein (isoform 3) has a distinct and longer C-terminus when compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic and transcript sequence to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.