VMAT1 (SLC18A1) (NM_001142325) Human Untagged Clone

CAT#: SC325972

SLC18A1 (untagged)-Human solute carrier family 18 (vesicular monoamine), member 1 (SLC18A1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC18A1
• Synonyms: CGAT; VAT1; VMAT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001142325, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTCCGGACCATTCTGGATGCTCCCCAGCGGTTGCTGAAGGAGGGGAGAGCGTCCCGGCAGCTGGTGC
  TGGTGGTGGTATTCGTCGCTTTGCTCCTGGACAACATGCTGTTTACTGTGGTGGTGCCAATTGTGCCCAC
  CTTCCTATATGACATGGAGTTCAAAGAAGTCAACTCTTCTCTGCACCTCGGCCATGCCGGAAGTTCCCCA
  CATGCCCTCGCCTCTCCTGCCTTTTCCACCATCTTCTCCTTCTTCAACAACAACACCGTGGCTGTTGAAG
  AAAGCGTACCTAGTGGAATAGCATGGATGAATGACACTGCCAGCACCATCCCACCTCCAGCCACTGAAGC
  CATCTCAGCTCATAAAAACAACTGCTTGCAAGGCACAGGTTTCTTGGAGGAAGAGATTACCCGGGTCGGG
  GTTCTGTTTGCTTCAAAGGCTGTGATGCAACTTCTGGTCAACCCATTCGTGGGCCCTCTCACCAACAGGA
  TTGGATATCATATCCCCATGTTTGCTGGCTTTGTTATCATGTTTCTCTCCACAGTTATGTTTGCTTTTTC
  TGGGACCTATACTCTACTCTTTGTGGCCCGAACCCTTCAAGGCATTGGATCTTCATTTTCATCTGTTGCA
  GGTCTTGGAATGCTGGCCAGTGTCTACACTGATGACCATGAGAGAGGACGAGCCATGGGAACTGCTCTGG
  GGGGCCTGGCCTTGGGGTTGCTGGTGGGAGCTCCCTTTGGAAGTGTAATGTACGAGTTTGTTGGGAAGTC
  TGCACCCTTCCTCATCCTGGCCTTCCTGGCACTACTGGATGGAGCACTCCAGCTTTGCATCCTACAGCCT
  TCCAAAGTCTCTCCTGAGAGTGCCAAGGGGACTCCCCTCTTTATGCTTCTCAAAGACCCTTACATCCTGG
  TGGCTGCAGGGTCCATCTGCTTTGCCAACATGGGGGTGGCCATCCTGGAGCCCACACTGCCCATCTGGAT
  GATGCAGACCATGTGCTCCCCCAAGTGGCAGCTGGGTCTAGCTTTCTTGCCTGCCAGTGTGTCCTACCTC
  ATTGGCACCAACCTCTTTGGTGTGTTGGCCAACAAGATGGGTCGGTGGCTGTGTTCCCTAATCGGGATGC
  TGGTAGTAGGTACCAGCTTGCTCTGTGTTCCTCTGGCTCACAATATTTTTGGTCTCATTGGCCCCAATGC
  AGGGCTTGGCCTTGCCATAGGCATGGTGGATTCTTCTATGATGCCCATCATGGGGCACCTGGTGGATCTA
  CGCCACACCTCGGTGTATGGGAGTGTCTACGCCATCGCTGATGTGGCTTTTTGCATGGGCTTTGCTATAG
  GCTATTCTGAGTCAGGACTGCCCCATGGAGACCCGGATGTATGCAACCCAGAAGCCCACGAAGGAATTTC
  CTCTGGGGGAGGACAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142325
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142325.1, NP_001135797.1
• RefSeq Size: 2840 bp
• RefSeq ORF: 1419 bp
• Locus ID: 6570
• Cytogenetics: 8p21.3
• Protein Families: Transmembrane
• Protein Pathways: Parkinson's disease
• Gene Summary: 'The vesicular monoamine transporter acts to accumulate cytosolic monoamines into vesicles, using the proton gradient maintained across the vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs, including reserpine and tetrabenazine (Peter et al., 1993 [PubMed 7905859]). See also SLC18A2 (MIM 193001).[supplied by OMIM, Mar 2008]'
  Transcript Variant: This variant (4), also known as VMAT1delta15, lacks an alternate exon in the 3' coding region resulting in a frameshift, compared to variant 1. The resulting isoform (c) has a shorter and distinct C-terminus, compared to isoform a. CCDS Note: This variant represents the VMAT1delta15 variant described in PMID:16326835. There are no publicly available transcripts representing it, but it was cloned and characterized in the above publication.