IMPDH1 (NM_001142576) Human Untagged Clone

CAT#: SC326072

IMPDH1 (untagged)-Human IMP (inosine 5'-monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IMPDH1
• Synonyms: IMPD; IMPD1; IMPDH-I; LCA11; RP10; sWSS2608
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001142576, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGGGCCACTCACTCCACCACCGCTGCAGGGAGGCGGAGCCGCCGCTGTTCCGGAGCCCGGAGCCC
  GGCAACACCCGGGACACGAGACGGCGGCGCAGCGGTACAGCGCCCGACTGCTGCAGGCCGGCTACGAGCC
  CGAGAGCCCTAGATTGGACCTCGCTACACACCCGACGACACCCCGTTCAGAACTATCTTCAGTGGTCTTA
  CTGGCAGGTGTTGGTGTCCAGATGGATCGCCTTCGCAGGGCTAGCGACTTCCTGATTCTCCCAGGATTCA
  TAGACTTCATAGCTGATGAGGTGGACCTGACCTCAGCCCTGACCCGGAAGATCACGCTGAAGACGCCACT
  GATCTCCTCCCCCATGGACACTGTGACAGAGGCTGACATGGCCATTGCCATGGCTCTGATGGGAGGTATT
  GGTTTCATTCACCACAACTGCACCCCAGAGTTCCAGGCCAACGAGGTGCGGAAGGTCAAGAAGTTTGAAC
  AGGGCTTCATCACGGACCCTGTGGTGCTGAGCCCCTCGCACACTGTGGGCGATGTGCTGGAGGCCAAGAT
  GCGGCATGGCTTCTCTGGCATCCCCATCACTGAGACGGGCACCATGGGCAGCAAGCTGGTGGGCATCGTC
  ACCTCCCGAGACATCGACTTTCTTGCTGAGAAGGACCACACCACCCTCCTCAGTGAGGTGATGACGCCAA
  GGATTGAACTGGTGGTGGCTCCAGCAGGTGTGACGTTGAAAGAGGCAAATGAGATCCTGCAGCGTAGCAA
  GAAAGGGAAGCTGCCTATCGTCAATGATTGCGATGAGCTGGTGGCCATCATCGCCCGCACCGACCTGAAG
  AAGAACCGAGACTACCCTCTGGCCTCCAAGGATTCCCAGAAGCAGCTGCTCTGTGGGGCAGCTGTGGGCA
  CCCGTGAGGATGACAAATACCGTCTGGACCTGCTCACCCAGGCGGGCGTCGACGTCATAGTCTTGGACTC
  GTCCCAAGGGAATTCGGTGTATCAGATCGCCATGGTGCATTACATCAAACAGAAGTACCCCCACCTCCAG
  GTGATTGGGGGGAACGTGGTGACAGCAGCCCAGGCCAAGAACCTGATTGATGCTGGTGTGGACGGGCTGC
  GCGTGGGCATGGGCTGCGGCTCCATCTGCATCACCCAGGAAGTGATGGCCTGTGGTCGGCCCCAGGGCAC
  TGCTGTGTACAAGGTGGCTGAGTATGCCCGGCGCTTTGGTGTGCCCATCATAGCCGATGGCGGCATCCAG
  ACCGTGGGACACGTGGTCAAGGCCCTGGCCCTTGGAGCCTCCACAGTGATGATGGGCTCCCTGCTGGCCG
  CCACTACGGAGGCCCCTGGCGAGTACTTCTTCTCAGACGGGGTGCGGCTCAAGAAGTACCGGGGCATGGG
  CTCACTGGATGCCATGGAGAAGAGCAGCAGCAGCCAGAAACGATACTTCAGCGAGGGGGATAAAGTGAAG
  ATCGCGCAGGGTGTCTCGGGCTCCATCCAGGACAAAGGATCCATTCAGAAGTTCGTGCCCTACCTCATAG
  CAGGCATCCAACACGGCTGCCAGGATATCGGGGCCCGCAGCCTGTCTGTCCTTCGGTCCATGATGTACTC
  AGGAGAGCTCAAGTTTGAGAAGCGGACCATGTCGGCCCAGATTGAGGGTGGTGTCCATGGCCTGCACTCT
  TACGAAAAGCGGCTGTACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142576
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142576.1, NP_001136048.1
• RefSeq Size: 2526 bp
• RefSeq ORF: 1701 bp
• Locus ID: 3614
• Cytogenetics: 7q32.1
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism
• Gene Summary: 'The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]'
  Transcript Variant: This variant (4) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (dz) has the same N- and C-termini but is shorter compared to isoform a.