GGCX (NM_001142269) Human Untagged Clone

CAT#: SC326185

GGCX (untagged)-Human gamma-glutamyl carboxylase (GGCX), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GGCX
• Synonyms: VKCFD1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001142269, the custom clone sequence may differ by one or more nucleotides
  ATGGCGGTGTCTGCCGGGTCCGCGCGGACCTCGCCCAGCTCAGGGTTCTTGATGGTGCTA
  GACATTCCCCAGGAGCGGGGGCTCAGCTCTCTGGACCGGAAATACCTTGATGGGCTGGAT
  GTGTGCCGCTTCCCCTTGCTGGATGCCCTACGCCCACTGCCACTTGACTGGATGTATCTT
  GTCTACACCATCATGTTTCTGGGGGCACTGGGCATGATGCTGGGCCTGTGCTACCGGATA
  AGCTGTGTGTTATTCCTGCTGCCATACTGGTATGTGTTTCTCCTGGACAAGACATCATGG
  AACAACCACTCCTATCTGTATGGGTTGTTGGCCTTTCAGCTAACATTCATGGATGCAAAC
  CACTACTGGTCTGTGGACGGTCTGCTGAATGCCCATAGGAGGAATGCCCACGTGCCCCTT
  TGGAACTATGCAGTGCTCCGTGGCCAGATCTTCATTGTGTACTTCATTGCGGGTGTGAAA
  AAGCTGGATGCAGACTGGGTTGAAGGCTATTCCATGGAATATTTGTCCCGGCACTGGCTC
  TTCAGTCCCTTCAAACTGCTGTTGTCTGAGGAGCTGACTAGCCTGCTGGTCGTGCACTGG
  GGTGGGCTGCTGCTTGACCTCTCAGCTGGTTTCCTGCTCTTTTTTGATGTCTCAAGATCC
  ATTGGCCTGTTCTTTGTGTCCTACTTCCACTGCATGAATTCCCAGCTTTTCAGCATTGGT
  ATGTTCTCCTACGTCATGCTGGCCAGCAGCCCTCTCTTCTGCTCCCCTGAGTGGCCTCGG
  AAGCTGGTGTCCTACTGCCCCCGAAGGTTGCAACAACTGTTGCCCCTCAAGGCAGCCCCT
  CAGCCCAGTGTTTCCTGTGTGTATAAGAGGAGCCGGGGCAAAAGTGGCCAGAAGCCAGGG
  CTGCGCCATCAGCTGGGAGCTGCCTTCACCCTGCTCTACCTCCTGGAGCAGCTATTCCTG
  CCCTATTCTCATTTTCTCACCCAGGGCTATAACAACTGGACAAATGGGCTGTATGGCTAT
  TCCTGGGACATGATGGTGCACTCCCGCTCCCACCAGCACGTGAAGATCACCTACCGTGAT
  GGCCGCACTGGCGAACTGGGCTACCTTAACCCTGGGGTATTTACACAGAGTCGGCGATGG
  AAGGATCATGCAGACATGCTGAAGCAATATGCCACTTGCCTGAGCCGCCTGCTTCCCAAG
  TATAATGTCACTGAGCCCCAGATCTACTTTGATATTTGGGTCTCCATCAATGACCGCTTC
  CAGCAGAGGATTTTTGACCCTCGTGTGGACATCGTGCAGGCCGCTTGGTCACCCTTTCAG
  CGCACATCCTGGGTGCAACCACTCTTGATGGACCTGTCTCCCTGGAGGGCCAAGTTACAG
  GAAATCAAGAGCAGCCTAGACAACCACACTGAGGTGGTCTTCATTGCAGATTTCCCTGGA
  CTGCACTTGGAGAATTTTGTGAGTGAAGACCTGGGCAACACTAGCATCCAGCTGCTGCAG
  GGGGAAGTGACTGTGGAGCTTGTGGCAGAACAGAAGAACCAGACTCTTCGAGAGGGAGAA
  AAAATGCAGTTGCCTGCTGGTGAGTACCATAAGGTGTATACGACATCACCTAGCCCTTCT
  TGCTACATGTACGTCTATGTCAACACTACAGAGCTTGCACTGGAGCAAGACCTGGCATAT
  CTGCAAGAATTAAAGGAAAAGGTGGAGAATGGAAGTGAAACAGGGCCTCTACCCCCAGAG
  CTGCAGCCTCTGTTGGAAGGGGAAGTAAAAGGGGGCCCTGAGCCAACACCTCTGGTTCAG
  ACCTTTCTTAGACGCCAACAAAGGCTCCAGGAGATTGAACGCCGGCGAAATACTCCTTTC
  CATGAGCGATTCTTCCGCTTCTTGTTGCGAAAGCTCTATGTCTTTCGCCGCAGCTTCCTG
  ATGACTTGTATCTCACTTCGAAATCTGATATTAGGCCGTCCTTCCCTGGAGCAGCTGGCC
  CAGGAGGTGACTTATGCAAACTTGAGACCCTTTGAGGCAGTTGGAGAACTGAATCCCTCA
  AACACGGATTCTTCACATTCTAATCCTCCTGAGTCAAATCCTGATCCTGTCCACTCAGAG
  TTC
  
• Restriction Sites: Please inquire
• ACCN: NM_001142269
• Insert Size: 7444 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142269.1, NP_001135741.1
• RefSeq Size: 7444 bp
• RefSeq ORF: 2106 bp
• Locus ID: 2677
• Cytogenetics: 2p11.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes an integral membrane protein of the rough endoplasmic reticulum that carboxylates glutamate residues of vitamin K-dependent proteins to gamma carboxyl glutamate, a modification that is required for their activity. The vitamin K-dependent protein substrates have a propeptide that binds the enzyme, with carbon dioxide, dioxide, and reduced vitamin K acting as co-substrates. Vitamin K-dependent proteins affect a number of physiologic processes including blood coagulation, prevention of vascular calcification, and inflammation. Allelic variants of this gene have been associated with pseudoxanthoma elasticum-like disorder with associated multiple coagulation factor deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.