HNF1 beta (HNF1B) (NM_001165923) Human Untagged Clone

CAT#: SC327068

HNF1B (untagged)-Human HNF1 homeobox B (HNF1B) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HNF1B
• Synonyms: FJHN; HNF-1-beta; HNF-1B; HNF1beta; HNF2; HPC11; LF-B3; LFB3; MODY5; TCF-2; TCF2; VHNF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001165923, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGTCCAAGCTCACGTCGCTCCAGCAAGAACTCCTGAGCGCCCTGCTGAGCTCCGGGGTCACCAAGG
  AGGTGCTGGTTCAGGCCTTGGAGGAGTTGCTGCCATCCCCGAACTTCGGGGTGAAGCTGGAGACGCTGCC
  CCTGTCCCCTGGCAGCGGGGCCGAGCCCGACACCAAGCCGGTCTTCCATACTCTCACCAACGGCCACGCC
  AAGGGCCGCTTGTCCGGCGACGAGGGCTCCGAGGACGGCGACGACTATGACACACCTCCCATCCTCAAGG
  AGCTGCAGGCGCTCAACACCGAGGAGGCGGCGGAGCAGCGGGCGGAGGTGGACCGGATGCTCAGTGAGGA
  CCCTTGGAGGGCTGCTAAAATGATCAAGGGTTACATGCAGCAACACAACATCCCCCAGAGGGAGGTGGTC
  GATGTCACCGGCCTGAACCAGTCGCACCTCTCCCAGCATCTCAACAAGGGCACCCCTATGAAGACCCAGA
  AGCGTGCCGCTCTGTACACCTGGTACGTCAGAAAGCAACGAGAGATCCTCCGACAGTTCAGTCAACAGAG
  CCATGGGCCTGGGCAGTCCGATGATGCCTGCTCTGAGCCCACCAACAAGAAGATGCGCCGCAACCGGTTC
  AAATGGGGGCCCGCGTCCCAGCAAATCTTGTACCAGGCCTACGATCGGCAAAAGAACCCCAGCAAGGAAG
  AGAGAGAGGCCTTAGTGGAGGAATGCAACAGGGCAGAATGTTTGCAGCGAGGGGTGTCCCCCTCCAAAGC
  CCACGGCCTGGGCTCCAACTTGGTCACTGAGGTCCGTGTCTACAACTGGTTTGCAAACCGCAGGAAGGAG
  GAGGCATTCCGGCAAAAGCTGGCCATGGACGCCTATAGCTCCAACCAGACTCACAGCCTGAACCCTCTGC
  TCTCCCACGGCTCCCCCCACCACCAGCCCAGCTCCTCTCCTCCAAACAAGCTGTCAGGAGTGCGCTACAG
  CCAGCAGGGAAACAATGAGATCACTTCCTCCTCAACAATCAGTCACCATGGCAACAGCGCCATGGTGACC
  AGCCAGTCGGTTTTACAGCAAGTCTCCCCAGCCAGCCTGGACCCAGGCCACAATCTCCTCTCACCTGATG
  GTAAAATGATCTCAGTCTCAGGAGGAGGTTTGCCCCCAGTCAGCACCTTGACGAATATCCACAGCCTCTC
  CCACCATAATCCCCAGCAATCTCAAAACCTCATCATGACACCCCTCTCTGGAGTCATGGCAATTGCACAA
  AGCCTCAACACCTCCCAAGCACAGAGTGTCCCTGTCATCAACAGTGTGGCCGGCAGCCTGGCAGCCCTGC
  AGCCCGTCCAGTTCTCCCAGCAGCTGCACAGCCCTCACCAGCAGCCCCTCATGCAGCAGAGCCCAGGCAG
  CCACATGGCCCAGCAGCCCTTCATGGCAGCTGTGACTCAGCTGCAGAACTCACACATGTACGCACACAAG
  CAGGAACCCCCCCAGTATTCCCACACCTCCCGGTTTCCATCTGCAATGGTGGTCACAGATACCAGCAGCA
  TCAGTACACTCACCAACATGTCTTCAAGTAAACAGTGTCCTCTACAAGCCTGGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001165923
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001165923.3, NP_001159395.1
• RefSeq Size: 2746 bp
• RefSeq ORF: 1596 bp
• Locus ID: 6928
• Cytogenetics: 17q12
• Protein Families: Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: 'This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.