EPS15 (NM_001159969) Human Untagged Clone

CAT#: SC327109

EPS15 (untagged)-Human epidermal growth factor receptor pathway substrate 15 (EPS15) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EPS15
• Synonyms: AF-1P; AF1P; MLLT5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001159969, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTATTTAAAAAGCGATTCAGGATTAGGAGGATGGATAACCATACCAGCTGTAGCTGATGTTCTAAAAT
  ATTCTTGCATTGTTTGCTGGTCTTCTAGGGAAAAGAATAATGTGGAACAGGACCTTAAGGAGAAGGAAGA
  TACTATTAAACAGAGGACAAGTGAGGTTCAGGATCTTCAAGATGAAGTTCAAAGGGAGAATACTAATCTG
  CAAAAACTACAGGCCCAGAAACAGCAGGTACAGGAACTCCTTGATGAACTGGATGAGCAGAAAGCCCAGC
  TGGAGGAGCAACTCAAGGAAGTCAGAAAGAAATGTGCTGAGGAGGCCCAACTGATCTCTTCTCTGAAAGC
  TGAATTAACTAGTCAGGAATCGCAGATCTCCACTTACGAAGAAGAATTGGCAAAAGCTAGAGAAGAGCTG
  AGCCGTCTACAGCAAGAAACAGCAGAATTGGAGGAGAGTGTAGAGTCAGGGAAGGCTCAGTTGGAACCTC
  TTCAGCAGCACCTACAAGATTCACAACAGGAAATTAGTTCAATGCAAATGAAACTGATGGAAATGAAAGA
  TTTGGAAAATCATAATAGTCAGTTAAATTGGTGCAGTAGCCCACACAGCATTCTTGTAAACGGAGCTACA
  GATTATTGCAGCCTCAGCACCAGCAGCAGTGAAACAGCCAACCTTAATGAACATGTTGAAGGCCAGAGCA
  ACCTAGAGTCTGAGCCCATACACCAGGAATCTCCAGCAAGAAGTAGTCCTGAACTACTGCCTTCTGGTGT
  GACTGATGAAAATGAGGTGACTACAGCTGTTACTGAAAAAGTTTGTTCTGAACTCGACAATAATAGACAT
  TCAAAAGAGGAAGATCCATTTAATGTAGACTCAAGTTCGCTGACAGGTCCAGTTGCAGATACAAACTTGG
  ATTTTTTCCAGTCTGATCCTTTTGTTGGCAGTGATCCTTTCAAGGATGATCCTTTTGGAAAAATCGATCC
  ATTTGGTGGTGATCCTTTCAAAGGTTCAGATCCATTTGCATCAGACTGTTTCTTCAGGCAATCTACTGAT
  CCTTTTGCCACTTCAAGCACTGACCCTTTCAGTGCAGCCAACAATAGCAGTATTACATCGGTAGAAACGT
  TGAAGCACAATGATCCTTTTGCTCCTGGTGGAACAGTTGTTGCAGCAAGCGATTCAGCCACAGACCCCTT
  TGCTTCTGTTTTTGGGAATGAATCATTTGGAGGTGGATTTGCTGACTTCAGCACATTGTCAAAGGTCAAC
  AATGAAGATCCTTTTCGTTCAGCCACATCGAGCTCTGTCAGCAACGTAGTGATTACAAAAAATGTATTTG
  AGGAAACATCGGTCAAAAGTGAAGATGAACCCCCAGCACTGCCACCAAAGATCGGAACTCCAACAAGACC
  CTGCCCTCTACCACCTGGGAAAAGATCCATCAACAAATTGGATTCTCCTGATCCCTTTAAACTGAATGAT
  CCATTTCAGCCTTTCCCAGGCAACGATAGCCCCAAAGAAAAAGATCCTGAAATATTTTGTGATCCATTCA
  CTTCTGCTACTACCACTACCAATAAAGAGGCTGATCCAAGCAATTTTGCCAACTTCAGTGCTTATCCCTC
  TGAAGAAGATATGATCGAATGGGCCAAGAGGGAAAGTGAGAGAGAGGAAGAGCAGAGGCTTGCCCGACTA
  AATCAGCAGGAACAAGAAGACTTAGAACTGGCTATTGCACTCAGCAAATCTGAGATATCAGAAGCATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159969
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159969.1, NP_001153441.1
• RefSeq Size: 4351 bp
• RefSeq ORF: 1749 bp
• Locus ID: 2060
• Cytogenetics: 1p32.3
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• Gene Summary: 'This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009]'
  Transcript Variant: This variant (2) lacks multiple exons at the 5' end and has a distinct 5' UTR and start codon, compared to variant 1. These differences result in a protein (isoform B) with a shorter and distinct N-terminus, compared to isoform A. Isoform B localizes to early endosome membranes.