THEMIS (NM_001164685) Human Untagged Clone

CAT#: SC327166

THEMIS (untagged)-Human thymocyte selection associated (THEMIS) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: THEMIS
• Synonyms: C6orf190; C6orf207; GASP; SPOT; TSEPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001164685, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCATTATCACTGGAAGAATTCGTCCACTCCCTTGACCTCAGGACCCTACCCAGGGTTCTAGAAATCC
  AGGCAGGCATCTATCTTGAAGGCTCTATTTATGAAATGTTTGGAAATGAATGCTGTTTTTCAACAGGAGA
  AGTGATTAAAATTACTGGTCTCAAAGTTAAGAAGATCATAGCTGAAATTTGTGAGCAGATTGAAGGTTGT
  GAGTCTCTACAGCCATTTGAACTGCCTATGAATTTTCCAGGTCTTTTTAAGATTGTGGCTGATAAAACTC
  CATACCTTACTATGGAAGAAATCACAAGGACCATTCATATTGGACCAAGTAGACTAGGGCATCCTTGCTT
  CTATCATCAGAAGGATATAAAACTAGAGAACCTCATCATAAAGCAGGGTGAGCAAATCATGCTCAACTCA
  GTTGAAGAGATTGATGGAGAAATAATGGTGAGCTGTGCAGTAGCAAGGAATCATCAAACTCACTCATTTA
  ATTTGCCTTTGTCACAAGAAGGAGAATTCTACGAGTGTGAAGATGAACGTATTTACACTCTAAAGGAGAT
  TGTTGAATGGAAGATTCCTAAGAACAGAACAAGAACTGTAAACCTTACAGATTTTTCAAATAAGTGGGAC
  TCAACGAATCCATTTCCTAAAGACTTTTATGGTACCCTGATTCTCAAGCCTGTTTATGAAATTCAAGGTG
  TGATGAAATTTCGAAAAGATATAATCCGCATCCTCCCCAGTCTAGATGTCGAAGTCAAAGACATCACTGA
  TTCTTACGATGCTAACTGGTTTCTTCAGCTGTTATCAACAGAAGATCTTTTTGAAATGACTAGTAAAGAG
  TTCCCCATAGTGACTGAAGTCATAGAAGCACCTGAAGGAAACCACCTGCCCCAAAGCATTTTACAGCCTG
  GGAAAACCATTGTGATCCACAAAAAGTACCAGGCATCAAGAATCTTAGCTTCAGAAATTAGAAGCAATTT
  TCCTAAAAGACACTTCTTGATCCCCACTAGCTATAAAGGCAAGTTCAAGCGGCGACCGAGGGAGTTCCCA
  ACGGCCTATGACCTAGAGATCGCTAAGAGTGAAAAGGAGCCTCTTCACGTGGTGGCCACCAAAGCGTTTC
  ATTCCCCTCATGACAAGCTGTCATCCGTATCTGTTGGGGACCAGTTTCTGGTGCATCAGTCAGAGACGAC
  TGAAGTCCTCTGTGAGGGAATAAAAAAAGTGGTGAATGTTCTGGCCTGTGAAAAAATCCTCAAAAAGTCC
  TATGAGGCTGCGCTGCTCCCTTTGTACATGGAAGGAGGTTTTGTAGAGGTGATTCATGATAAGAAACAGT
  ACCCGATTTCTGAGCTCTGTAAACAGTTCCGTTTGCCCTTCAATGTGAAGGTGTCTGTCAGGGATCTTTC
  CATTGAAGAGGACGTGTTGGCTGCCACACCAGGACTGCAGTTGGAGGAGGACATTACAGACTCTTACCTA
  CTCATAAGTGACTTTGCCAACCCCACGGAGTGCTGGGAAATTCCTGTGGGCCGCTTGAATATGACTGTTC
  AGTTAGTTAGTAATTTCTCTAGGGATGCAGAACCATTTCTAGTCAGGACTCTGGTAGAAGAGATCACTGA
  AGAGCAATATTACATGATGCGGAGATATGAAAGCTCAGCCTCACATCCCCCACCTCGCCCTCCGAAACAC
  CCCTCAGTAGAGGAAACAAAGTTAACCCTGCTAACCTTAGCAGAAGAAAGGACGGTAGACCTGCCCAAGT
  CTCCCAAGGCTGGAGTGCAATGGCGTGATCTTGGCTCGCTGCAACCTCTGCCTCCCGGGTTCAAGCAATT
  CTCTGCCTCAGCCTCCCACGTAGCTGGGATTACAGGCACCCCCCACCACGTCCAGCGTCATCACGTAGAC
  ATAACCAAGAAACTTCACCCAAATCAAGCTGGCCTGGATTCAAAAGTACTGATTGGTAGTCAGAATGATT
  TGGTGGATGAAGAGAAAGAAAGGAGCAACCGTGGGGCCACAGCAATAGCAGAAACATTCAAAAATGAAAA
  ACATCAAAAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164685
• ORF Size: 2043 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001164685.1, NP_001158157.1
• RefSeq Size: 3983
• RefSeq ORF: 2043
• Locus ID: 387357
• Gene Summary: This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).