Prolactin (PRL) (NM_001163558) Human Untagged Clone

CAT#: SC327403

PRL (untagged)-Human prolactin (PRL) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRL
• Synonyms: GHA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000948 edited
  GGGATCCTTATTCTATATCTCTTGGTATTTAGTGTAAAAATTTTAAAATCTTTACCTAGC
  AATCTTGAGGAAGAAACTTGATAACTGATAATACATGAGATTGTTACCTAAGTGAAATAT
  AATCCTATATATTCAACAAACTTTAGAGAAATAAGATAAATTTTAAAGTAAATGACTTCT
  GTAGTTTTATAGATCCTCCAAACCAATCTAGTCTCAGATCTCACCTTCATCATTTCTCTC
  ATTTCCTTTTGGCCTAATTAATCAAAATCCTTCCTAGAATGTTCATTTCTGGCCAGTATG
  TCTTCCTGAATATGAATAAGAAATAAAATACCATTTGATGTTTGAAATTATGGGGGTAAT
  CTCAATGACGGAAATAGATGACCAGGAAAAGGGAAACGAATGCCTGATTCATTATATTCA
  TGAAGATATCAAAGGTTTATAAAGCCAATATCTGGGAAAGAGAAAACCGTGAGACTTCCA
  GATCTTCTCTGGTGAAGTGTGTTTCCTGCAACGATCACGAACATGAACATCAAAGGATCG
  CCATGGAAAGGGTCCCTCCTGCTGCTGCTGGTGTCAAACCTGCTCCTGTGCCAGAGCGTG
  GCCCCCTTGCCCATCTGTCCCGGCGGGGCTGCCCGATGCCAGGTGACCCTTCGAGACCTG
  TTTGACCGCGCCGTCGTCCTGTCCCACTACATCCATAACCTCTCCTCAGAAATGTTCAGC
  GAATTCGATAAACGGTATACCCATGGCCGGGGGTTCATTACCAAGGCCATCAACAGCTGC
  CACACTTCTTCCCTTGCCACCCCCGAAGACAAGGAGCAAGCCCAACAGATGAATCAAAAA
  GACTTTCTGAGCCTGATAGTCAGCATATTGCGATCCTGGAATGAGCCTCTGTATCATCTG
  GTCACGGAAGTACGTGGTATGCAAGAAGCCCCGGAGGCTATCCTATCCAAAGCTGTAGAG
  ATTGAGGAGCAAACCAAACGGCTTCTAGAGGGCATGGAGCTGATAGTCAGCCAGGTTCAT
  CCTGAAACCAAAGAAAATGAGATCTACCCTGTCTGGTCGGGACTTCCATCCCTGCAGATG
  GCTGATGAAGAGTCTCGCCTTTCTGCTTATTATAACCTGCTCCACTGCCTACGCAGGGAT
  TCACATAAAATCGACAATTATCTCAAGCTCCTGAAGTGCCGAATCATCCACAACAACAAC
  TGCTAAGCCCACATCCATTTCATCTATTTCTGAGAAGGTCCTTAATGATCCGTTCCATTG
  CAAGCTTCTTTTAGTTGTATCTCTTTTGAATCCATGCTTGGGTGTAACAGGTCTCCTCTT
  AAAAAATAAAAACTGACTCCTTAGAGACATCAAAATCCAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001163558
• Insert Size: 1400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163558.1, NP_001157030.1
• RefSeq Size: 1027 bp
• RefSeq ORF: 684 bp
• Locus ID: 5617
• Cytogenetics: 6p22.3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.