alpha 1 Glycine Receptor (GLRA1) (NM_001146040) Human Untagged Clone

CAT#: SC327542

GLRA1 (untagged)-Human glycine receptor alpha 1 (GLRA1) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLRA1
• Synonyms: HKPX1; STHE
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001146040 edited
  CAACAGACACGCTGGAGTTTAACAAACAGCAATACTCTTCGCGCTCCTGAAAAGCAGGTC
  TGGACGCTCTCCGTGGTGCTGAAACGCCTCGTAGCCGCCGCTGTCCGTGGTATCTACGAC
  CCCCTCGCTCCAATTTCCCCTGGGGCTCTCCCTCCGCGCCCCTGTTCCCCGCCTCCCTTT
  AACATCTGGATTATTTTTTGCAATAGCGCTTTCTGGTTTTGTAAGTGCCAATTTGAAACA
  TTTTTGCCCCCATAACTCGTGGACTACAAAGCACAAGGACCTGAAAAATGTACAGCTTCA
  ATACTCTTCGACTCTACCTTTGGGAGACCATTGTATTCTTCAGCCTTGCTGCTTCTAAGG
  AGGCTGAAGCTGCTCGCTCCGCACCCAAGCCTATGTCACCCTCGGATTTCCTGGATAAGC
  TAATGGGGAGAACCTCCGGATATGATGCCAGGATCAGGCCCAATTTTAAAGGTCCCCCAG
  TGAACGTGAGCTGCAACATTTTCATCAACAGCTTTGGTTCCATTGCTGAGACAACCATGG
  ACTATAGGGTCAACATCTTCCTGCGGCAGCAATGGAACGACCCCCGCCTGGCCTATAATG
  AATACCCTGACGACTCTCTGGACCTGGACCCATCCATGCTGGACTCCATCTGGAAACCTG
  ACCTGTTCTTTGCCAACGAGAAGGGGGCCCACTTCCATGAGATCACCACAGACAACAAAT
  TGCTAAGGATCTCCCGGAATGGGAATGTCCTCTACAGCATCAGAATCACCCTGACACTGG
  CCTGCCCCATGGACTTGAAGAATTTCCCCATGGATGTCCAGACATGTATCATGCAACTGG
  AAAGCTTTGGATATACGATGAATGACCTCATCTTTGAGTGGCAGGAACAGGGAGCCGTGC
  AGGTAGCAGATGGACTAACTCTGCCCCAGTTTATCTTGAAGGAAGAGAAGGACTTGAGAT
  ACTGCACCAAGCACTACAACACAGGTAAATTCACCTGCATTGAGGCCCGGTTCCACCTGG
  AGCGGCAGATGGGTTACTACCTGATTCAGATGTATATTCCCAGCCTGCTCATTGTCATCC
  TCTCATGGATCTCCTTCTGGATCAACATGGATGCTGCACCTGCTCGTGTGGGCCTAGGCA
  TCACCACTGTGCTCACCATGACCACCCAGAGCTCCGGCTCTCGAGCATCTCTGCCCAAGG
  TGTCCTATGTGAAAGCCATTGACATTTGGATGGCAGTTTGCCTGCTCTTTGTGTTCTCAG
  CCCTATTAGAATATGCTGCCGTTAACTTTGTGTCTCGGCAACATAAGGAGCTGCTCCGAT
  TCAGGAGGAAGCGGAGACATCACAAGAGCCCCATGTTGAATCTATTCCAGGAGGATGAAG
  CTGGAGAAGGCCGCTTTAACTTCTCTGCCTATGGGATGGGCCCAGCCTGTCTACAGGCCA
  AGGATGGCATCTCAGTCAAGGGCGCCAACAACAGTAACACCACCAACCCCCCTCCTGCAC
  CATCTAAGTCCCCAGAGGAGATGCGAAAACTCTTCATCCAGAGGGCCAAGAAGATCGACA
  AAATATCCCGCATTGGCTTCCCCATGGCCTTCCTCATTTTCAACATGTTCTACTGGATCA
  TCTACAAGATTGTCCGTAGAGAGGACGTCCACAACCAGTGAAGGGTCTGAAAGGTTGGGG
  GAGGCTGGGAGAGGGGAACGTGGGAAT
  
• Restriction Sites: Please inquire
• ACCN: NM_001146040
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146040.1, NP_001139512.1
• RefSeq Size: 1835 bp
• RefSeq ORF: 1374 bp
• Locus ID: 2741
• Cytogenetics: 5q33.1
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'The protein encoded by this gene is a subunit of a pentameric inhibitory glycine receptor, which mediates postsynaptic inhibition in the central nervous system. Defects in this gene are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Multiple transcript variants encoding different isoforms have been found. [provided by RefSeq, Dec 2015]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).