AP1B1 (NM_001166019) Human Untagged Clone

CAT#: SC327674

AP1B1 (untagged)-Human adaptor-related protein complex 1 beta 1 subunit (AP1B1) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP1B1
• Synonyms: ADTB1; AP105A; BAM22; CLAPB2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC327674 sequence for NM_001166019 edited (data generated by NextGen Sequencing)
  
  
  ATGACTGACTCAAAATATTTCACCACGACCAAGAAAGGGGAGATCTTCGAGCTGAAGGCAGAGCTCAACA
  GTGACAAGAAGGAGAAGAAGAAGGAGGCAGTGAAGAAAGTGATTGCATCGATGACCGTGGGCAAAGATGT
  CAGTGCCCTCTTCCCCGATGTGGTCAACTGCATGCAGACGGACAACCTGGAGCTGAAGAAGCTGGTATAC
  CTCTACTTGATGAATTACGCCAAGAGTCAGCCTGACATGGCCATTATGGCCGTCAACACCTTTGTGAAGG
  ACTGTGAGGACCCCAACCCCCTCATCCGAGCCCTGGCAGTGCGGACCATGGGCTGCATCCGCGTTGACAA
  GATCACAGAGTACCTGTGCGAGCCACTCCGGAAGTGCCTGAAGGACGAGGATCCATATGTGCGCAAGACA
  GCAGCTGTGTGCGTGGCCAAGCTCCACGACATCAACGCCCAGCTGGTGGAGGACCAGGGCTTCCTGGACA
  CCCTTAAAGACCTCATCTCCGACTCTAACCCCATGGTGGTGGCCAATGCAGTGGCAGCGCTCTCAGAAAT
  TGCCGAGTCTCACCCCAGCAGCAACCTGCTCGATCTGAACCCACAGTCCATCAACAAGCTGCTGACAGCC
  CTCAATGAGTGCACCGAGTGGGGCCAGATCTTCATCCTGGACTGCCTCGCCAACTATATGCCCAAGGACG
  ACCGCGAGGCCCAGAGCATCTGTGAGCGGGTCACCCCCAGGCTCTCCCATGCCAACTCCGCTGTGGTGCT
  CTCTGCTGTGAAGGTGCTGATGAAGTTCATGGAGATGTTGTCTAAGGACTTGGACTACTACGGCACACTG
  CTCAAGAAGCTGGCCCCACCCCTGGTCACACTGCTGTCAGCCGAGCCAGAGCTGCAGTATGTGGCCCTGC
  GCAACATCAATCTCATCGTGCAGAAAAGGCCTGAGATCCTGAAGCATGAGATGAAGGTGTTCTTCGTGAA
  GTACAACGACCCTATCTACGTGAAGCTGGAGAAGCTGGACATCATGATCCGCCTGGCCTCTCAGGCCAAC
  ATCGCCCAGGTGTTGGCAGAGCTGAAAGAGTACGCAACAGAAGTGGATGTGGACTTTGTACGGAAGGCTG
  TGCGTGCTATTGGCCGCTGCGCCATCAAGGTGGAGCAATCTGCGGAGCGCTGTGTGAGCACGCTGCTCGA
  CCTCATCCAGACCAAGGTCAACTATGTGGTCCAGGAGGCCATCGTGGTCATCAAGGACATCTTCCGCAAG
  TACCCCAACAAGTATGAGAGTGTGATTGCCACACTGTGTGAGAATCTGGACTCCCTGGATGAGCCTGAGG
  CCCGGGCTGCCATGATCTGGATTGTGGGCGAGTACGCGGAACGGATCGACAACGCAGATGAGCTGCTGGA
  GAGCTTCCTCGAGGGCTTCCATGACGAGAGCACACAGGTCCAGCTGCAGCTGCTGACAGCCATTGTGAAA
  CTCTTTCTAAAGAAGCCAACAGAGACCCAGGAGCTGGTGCAGCAGGTCCTCAGTTTGGCCACTCAGGACT
  CAGATAACCCAGACCTGCGGGACCGTGGCTACATCTACTGGCGCCTGCTGTCCACGGACCCGGTGGCAGC
  CAAGGAGGTGGTGTTGGCTGAGAAGCCACTCATCTCTGAAGAGACGGACCTCATCGAGCCCACACTGTTA
  GACGAGCTTATCTGCTACATCGGCACGCTGGCTTCCGTCTACCATAAGCCTCCCAGTGCCTTTGTGGAGG
  GGGGCCGGGGCGTCGTGCACAAGAGCCTGCCACCTCGCACGGCCTCGAGTGAGAGCGCAGAGAGCCCTGA
  GACAGCCCCTACTGGAGCACCTCCTGGGGAGCAGCCAGATGTCATCCCCGCCCAGGGCGACCTGCTGGGT
  GACCTCCTCAACCTGGACCTCGGCCCCCCAGTGAGCGGCCCACCCCTGGCCACCTCCTCGGTGCAGATGG
  GAGCTGTGGACCTTCTTGGCGGTGGCCTTGACAGCCTGATTGGGGGCACCAACTTCGTGGCACCTCCAAC
  AGCAGCAGTACCAGCCAATCTTGGAGCACCCATCGGCAGTGGCCTGAGTGACCTCTTTGACCTGACCAGT
  GGCGTGGGCACCCTGTCAGGATCATATGTGGCCCCCAAAGCAGTGGGCTCCATCTCCATGGACCTGCAGC
  TGACCAACAAGGCCTTGCAGGTCATGACCGACTTTGCCATCCAGTTCAACCGCAACAGCTTTGGCCTGGC
  CCCCGCCGCCCCCCTCCAGGTCCACGCACCACTCAGCCCCAACCAGACAGTGGAGATCTCCCTGCCTCTC
  AGCACGGTGGGCTCGGTCATGAAGATGGAGCCTCTGAACAACCTCCAGGTGGCCGTGAAGAACAACATCG
  ATGTCTTCTACTTCAGCACCTTGTACCCACTGCACATCCTCTTTGTGGAGGACGGGAAGATGGACCGGCA
  GATGTTCCTGGCCACATGGAAGGATATTCCCAATGAGAATGAGGCCCAGTTCCAGATCAGAGACTGCCCC
  CTCAATGCAGAGGCTGCGAGCAGCAAGCTGCAGAGCAGCAACATCTTCACTGTCGCCAAGAGGAACGTGG
  AGGGCCAGGACATGCTCTACCAGTCCCTGAAGCTGACCAACGGCATCTGGGTGCTGGCGGAGCTGCGGAT
  CCAGCCGGGCAACCCCAGCTGCACGCTGTCCCTGAAGTGTCGAGCACCAGAGGTGTCCCAGCACGTGTAC
  CAGGCCTACGAGACCATCCTCAAGAACTGA
  
  Clone variation with respect to NM_001166019.1
  204:g=>a 2268:a=>g
• Restriction Sites: Please inquire
• ACCN: NM_001166019
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166019.1, NP_001159491.1
• RefSeq Size: 4104 bp
• RefSeq ORF: 2760 bp
• Locus ID: 162
• Cytogenetics: 22q12.2
• Protein Pathways: Lysosome
• Gene Summary: 'Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]'
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region, and lacks an alternate in-frame exon in both the central and 3' coding regions, compared to variant 1, resulting in an isoform (c) that is shorter than isoform a.