CACNA1C (NM_199460) Human Untagged Clone

CAT#: SC328101

CACNA1C (untagged)-Human calcium channel voltage-dependent L type alpha 1C subunit (CACNA1C) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CACNA1C
• Synonyms: CACH2; CACN2; CACNL1A1; CaV1.2; CCHL1A1; LQT8; TS; TS. LQT8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_199460 edited
  GAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTCTTCGTGGCTGCTCCTCCTAT
  TAAAACCATTTTTGGTCCATGGTCAATGAGAATACGAGGATGTACATTCCAGAGGAAAACCACCAAGGTT
  CCAACTATGGGAGCCCACGCCCCGCCCATGCCAACATGAATGCCAATGCGGCAGCGGGGCTGGCCCCTGA
  GCACATCCCCACCCCGGGGGCTGCCCTGTCGTGGCAGGCGGCCATCGACGCAGCCCGGCAGGCTAAGCTG
  ATGGGCAGCGCTGGCAATGCGACCATCTCCACAGTCAGCTCCACGCAGCGGAAGCGGCAGCAATATGGGA
  AACCCAAGAAGCAGGGCAGCACCACGGCCACACGCCCGCCCCGAGCCCTGCTCTGCCTGACCCTGAAGAA
  CCCCATCCGGAGGGCCTGCATCAGCATTGTCGAATGGAAACCATTTGAAATAATTATTTTACTGACTATT
  TTTGCCAATTGTGTGGCCTTAGCGATCTATATTCCCTTTCCAGAAGATGATTCCAACGCCACCAATTCCA
  ACCTGGAACGAGTGGAATATCTCTTTCTCATAATTTTTACGGTGGAAGCGTTTTTAAAAGTAATCGCCTA
  TGGACTCCTCTTTCACCCCAATGCCTACCTCCGCAACGGCTGGAACCTACTAGATTTTATAATTGTGGTT
  GTGGGGCTTTTTAGTGCAATTTTAGAACAAGCAACCAAAGCAGATGGGGCAAACGCTCTCGGAGGGAAAG
  GGGCCGGATTTGATGTGAAGGCGCTGAGGGCCTTCCGCGTGCTGCGCCCCCTGCGGCTGGTGTCCGGAGT
  CCCAAGTCTCCAGGTGGTCCTGAATTCCATCATCAAGGCCATGGTCCCCCTGCTGCACATCGCCCTGCTT
  GTGCTGTTTGTCATCATCATCTACGCCATCATCGGCTTGGAGCTCTTCATGGGGAAGATGCACAAGACCT
  GCTACAACCAGGAGGGCATAGCAGATGTTCCAGCAGAAGATGACCCTTCCCCTTGTGCGCTGGAAACGGG
  CCACGGGCGGCAGTGCCAGAACGGCACGGTGTGCAAGCCCGGCTGGGATGGTCCCAAGCACGGCATCACC
  AACTTTGACAACTTTGCCTTCGCCATGCTCACGGTGTTCCAGTGCATCACCATGGAGGGCTGGACGGACG
  TGCTGTACTGGGTCAATGATGCCGTAGGAAGGGACTGGCCCTGGATCTATTTTGTTACACTAATCATCAT
  AGGGTCATTTTTTGTACTTAACTTGGTTCTCGGTGTGCTTAGCGGAGAGTTTTCCAAAGAGAGGGAGAAG
  GCCAAGGCCCGGGGAGATTTCCAGAAGCTGCGGGAGAAGCAGCAGCTAGAAGAGGATCTCAAAGGCTACC
  TGGATTGGATCACTCAGGCCGAAGACATCGATCCTGAGAATGAGGACGAAGGCATGGATGAGGAGAAGCC
  CCGAAACATGAGCATGCCCACCAGTGAGACCGAGTCCGTCAACACCGAAAACGTGGCTGGAGGTGACATC
  GAGGGAGAAAACTGCGGGGCCAGGCTGGCCCACCGGATCTCCAAGTCAAAGTTCAGCCGCTACTGGCGCC
  GGTGGAATCGGTTCTGCAGAAGGAAGTGCCGCGCCGCAGTCAAGTCTAATGTCTTCTACTGGCTGGTGAT
  TTTCCTGGTGTTCCTCAACACGCTCACCATTGCCTCTGAGCACTACAACCAGCCCAACTGGCTCACAGAA
  GTCCAAGACACGGCAAACAAGGCCCTGCTGGCCCTGTTCACGGCAGAGATGCTCCTGAAGATGTACAGCC
  TGGGCCTGCAGGCCTACTTCGTGTCCCTCTTCAACCGCTTTGACTGCTTCGTCGTGTGTGGCGGCATCCT
  GGAGACCATCCTGGTGGAGACCAAGATCATGTCCCCACTGGGCATCTCCGTGCTCAGATGCGTCCGGCTG
  CTGAGGATTTTCAAGATCACGAGGTACTGGAACTCCTTGAGCAACCTGGTGGCATCCTTGCTGAACTCTG
  TGCGCTCCATCGCCTCCCTGCTCCTTCTCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGGGGATGCA
  GCTCTTTGGAGGAAAGTTCAACTTTGATGAGATGCAGACCCGGAGGAGCACATTCGATAACTTCCCCCAG
  TCCCTCCTCACTGTGTTTCAGATCCTGACCGGGGAGGACTGGAATTCGGTGATGTATGATGGGATCATGG
  CTTATGGCGGCCCCTCTTTTCCAGGGATGTTAGTCTGTATTTACTTCATCATCCTCTTCATCTGTGGAAA
  CTATATCCTACTGAATGTGTTCTTGGCCATTGCTGTGGACAACCTGGCTGATGCTGAGAGCCTCACATCT
  GCCCAAAAGGAGGAGGAAGAGGAGAAGGAGAGAAAGAAGCTGGCCAGGACTGCCAGCCCAGAGAAGAAAC
  AAGAGTTGGTGGAGAAGCCGGCAGTGGGGGAATCCAAGGAGGAGAAGATTGAGCTGAAATCCATCACGGC
  TGATGGAGAGTCTCCACCCGCCACCAAGATCAACATGGATGACCTCCAGCCCAATGAAAATGAGGATAAG
  AGCCCCTACCCCAACCCAGAAACTACAGGAGAAGAGGATGAGGAGGAGCCAGAGATGCCTGTCGGCCCTC
  GCCCACGACCACTCTCTGAGCTTCACCTTAAGGAAAAGGCAGTGCCCATGCCAGAAGCCAGCGCGTTTTT
  CATCTTCAGCTCTAACAACAGGTTTCGCCTCCAGTGCCACCGCATTGTCAATGACACGATCTTCACCAAC
  CTGATCCTCTTCTTCATTCTGCTCAGCAGCATTTCCCTGGCTGCTGAGGACCCGGTGCAGCACACCTCCT
  TCAGGAACCATATTCTGTTTTATTTTGATATTGTTTTTACCACCATTTTCACCATTGAAATTGCTCTGAA
  GATCCTAGGCAATGCAGACTATGTCTTCACTAGTATCTTTACATTAGAAATTATCCTTAAGATGACTGCT
  TATGGGGCTTTCTTGCACAAGGGTTCTTTCTGCCGGAACTACTTCAACATCCTGGACCTGCTGGTGGTCA
  GCGTGTCCCTCATCTCCTTTGGCATCCAGTCCAGTGCAATCAATGTCGTGAAGATCTTGCGAGTCCTGCG
  AGTACTCAGGCCCCTGAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAGTGTGTGTTTGTC
  GCCATCCGGACCATCGGGAACATCGTGATTGTCACCACCCTGCTGCAGTTCATGTTTGCCTGCATCGGGG
  TCCAGCTCTTCAAGGGAAAGCTGTACACCTGTTCAGACAGTTCCAAGCAGACAGAGGCGGAATGCAAGGG
  CAACTACATCACGTACAAAGACGGGGAGGTTGACCACCCCATCATCCAACCCCGCAGCTGGGAGAACAGC
  AAGTTTGACTTTGACAATGTTCTGGCAGCCATGATGGCCCTCTTCACCGTCTCCACCTTCGAAGGGTGGC
  CAGAGCTGCTGTACCGCTCCATCGACTCCCACACGGAAGACAAGGGCCCCATCTACAACTACCGTGTGGA
  GATCTCCATCTTCTTCATCATCTACATCATCATCATCGCCTTCTTCATGATGAACATCTTCGTGGGCTTC
  GTCATCGTCACCTTTCAGGAGCAGGGGGAGCAGGAGTACAAGAACTGTGAGCTGGACAAGAACCAGCGAC
  AGTGCGTGGAATACGCCCTCAAGGCCCGGCCCCTGCGGAGGTACATCCCCAAGAACCAGCACCAGTACAA
  AGTGTGGTACGTGGTCAACTCCACCTACTTCGAGTACCTGATGTTCGTCCTCATCCTGCTCAACACCATC
  TGCCTGGCCATGCAGCACTACGGCCAGAGCTGCCTGTTCAAAATCGCCATGAACATCCTCAACATGCTCT
  TCACTGGCCTCTTTACCGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGGGTTACTTTAGTGA
  TCCCTGGAATGTTTTTGACTTCCTCATCGTAATTGGCAGCATAATTGACGTCATTCTCAGTGAGACTAAT
  CACTATTTCTGTGATGCATGGAATACATTTGACGCCTTGATTGTTGTGGGTAGCATTGTTGATATAGCAA
  TCACCGAGGTAAACCCAGCTGAACATACCCAATGCTCTCCCTCTATGAACGCAGAGGAAAACTCCCGCAT
  CTCCATCACCTTCTTCCGCCTGTTCCGGGTCATGCGTCTGGTGAAGCTGCTGAGCCGTGGGGAGGGCATC
  CGGACGCTGCTGTGGACCTTCATCAAGTCCTTCCAGGCCCTGCCCTATGTGGCCCTCCTGATCGTGATGC
  TGTTCTTCATCTACGCGGTGATCGGGATGCAGGTGTTTGGGAAAATTGCCCTGAATGATACCACAGAGAT
  CAACCGGAACAACAACTTTCAGACCTTCCCCCAGGCCGTGCTGCTCCTCTTCAGGTGTGCCACCGGGGAG
  GCCTGGCAGGACATCATGCTGGCCTGCATGCCAGGCAAGAAGTGTGCCCCAGAGTCCGAGCCCAGCAACA
  GCACGGAGGGTGAAACACCCTGTGGTAGCAGCTTTGCTGTCTTCTACTTCATCAGCTTCTACATGCTCTG
  TGCCTTCCTGATCATCAACCTCTTTGTAGCTGTCATCATGGACAACTTTGACTACCTGACAAGGGACTGG
  TCCATCCTTGGTCCCCACCACCTGGATGAGTTTAAAAGAATCTGGGCAGAGTATGACCCTGAAGCCAAGG
  GTCGTATCAAACACCTGGATGTGGTGACCCTCCTCCGGCGGATTCAGCCGCCACTAGGTTTTGGGAAGCT
  GTGCCCTCACCGCGTGGCTTGCAAACGCCTGGTCTCCATGAACATGCCTCTGAACAGCGACGGGACAGTC
  ATGTTCAATGCCACCCTGTTTGCCCTGGTCAGGACGGCCCTGAGGATCAAAACAGAAGGGAACCTAGAAC
  AAGCCAATGAGGAGCTGCGGGCGATCATCAAGAAGATCTGGAAGCGGACCAGCATGAAGCTGCTGGACCA
  GGTGGTGCCCCCTGCAGGTGATGATGAGGTCACCGTTGGCAAGTTCTACGCCACGTTCCTGATCCAGGAG
  TACTTCCGGAAGTTCAAGAAGCGCAAAGAGCAGGGCCTTGTGGGCAAGCCCTCCCAGAGGAACGCGCTGT
  CTCTGCAGGCTGGCTTGCGCACACTGCATGACATCGGGCCTGAGATCCGACGGGCCATCTCTGGAGATCT
  CACCGCTGAGGAGGAGCTGGACAAGGCCATGAAGGAGGCTGTGTCCGCTGCTTCTGAAGATGACATCTTC
  AGGAGGGCCGGTGGCCTGTTCGGCAACCACGTCAGCTACTACCAAAGCGACGGCCGGAGCGCCTTCCCCC
  AGACCTTCACCACTCAGCGCCCGCTGCACATCAACAAGGCGGGCAGCAGCCAGGGCGACACTGAGTCGCC
  ATCCCACGAGAAGCTGGTGGACTCCACCTTCACCCCGAGCAGCTACTCGTCCACCGGCTCCAACGCCAAC
  ATCAACAACGCCAACAACACCGCCCTGGGTCGCCTCCCTCGCCCCGCCGGCTACCCCAGCACGGTCAGCA
  CTGTGGAGGGCCACGGGCCCCCCTTGTCCCCTGCCATCCGGGTGCAGGAGGTGGCGTGGAAGCTCAGCTC
  CAACAGGGAAAGGCACGTTCCGATGTGTGAGGATCTGGAGCTCAGGAGGGATTCAGGCTCAGCAGGGACT
  CAGGCTCACTGCCTTCTGCTCAGGAAAGCAAACCCCTCTAGGTGCCACTCCCGGGAGAGCCAGGCAGCCA
  TGGCGGGTCAGGAGGAGACGTCTCAGGATGAGATCTATGAAGTGAAGATGAACCATGACACGGAGGCCTG
  CAGTGAGCCCAGCCTGCTCTCCACAGAGATGCTCTCCTACCAGGATGACGAAAATCGGCAACTGACGCTC
  CCAGAGGAGGACAAGAGGGACATCCGGCAATCTCCGAAGAGGGGTTTCCTCCGCTCTGCCTCACTAGGTC
  GAAGGGCCTCCTTCCACCTGGAATGTCTGAAGCGACAGAAGGACCGAGGGGGAGACATCTCTCAGAAGAC
  AGTCCTGCCCTTGCATCTGGTTCATCATCAGGCATTGGCAGTGGCAGGCCTGAGCCCCCTCCTCCAGAGA
  AGCCATTCCCCTGCCTCATTCCCTAGGCCTTTTGCCACCCCACCAGCCACACCTGGCAGCCGAGGCTGGC
  CCCCACAGCCCGTCCCCACCCTGCGGCTTGAGGGGGTCGAGTCCAGTGAGAAACTCAACAGCAGCTTCCC
  ATCCATCCACTGCGGCTCCTGGGCTGAGACCACCCCCGGTGGCGGGGGCAGCAGCGCCGCCCGGAGAGTC
  CGGCCCGTCTCCCTCATGGTGCCCAGCCAGGCTGGGGCCCCAGGGAGGCAGTTCCACGGCAGTGCCAGCA
  GCCTGGTGGAAGCGGTCTTGATTTCAGAAGGACTGGGGCAGTTTGCTCAAGATCCCAAGTTCATCGAGGT
  CACCACCCAGGAGCTCGCCGACGCCTGCGACATGACCATAGAGGAGATGGAGAGCGCGGCCGACAACATC
  CTCAGCGGGGGCGCCCCACAGAGCCCCAATGGCGCCCTCTTACCCTTTGTGAACTGCAGGGACGCGGGGC
  AGGACCGAGCCGGGGGCGAAGAGGACGCGGGCTGTGTGCGCGCGCGGGGTCGACCGAGTGAGGAGGAGCT
  CCAGGACAGCAGGGTCTACGTCAGCAGCCTGTAGTGGGCGCTGCCAGATGCGGGCTTTTTTTTATTTGTT
  TCAATGTTCCTAATGGGTTCGTTTCAGAAGTGCCTCACTGTTCTCGTGACCTGGAGTTAACCGGAACAGC
  GTCTTCATTCATTTCTGTTGGGACCAGACGCGGAGCCTGGGTGCGCGAGCCGCCCTCCGGGAGGAAGGCG
  CCCGGCTGCGTCTGCAGAGGCGGGGAGAGGAGGCGGCGAGGGTCCCGGGGCGCGAGGAAGGCGCCTGCCC
  TCTCCCAGCTCGCAGGCCCCGGGCCCGGCCGCGCCTCTGCGGGGAGAGCACCCCGGCTTCCCGCGCGCCC
  TCACCAAAAGGACCCTACAGCAAACGGGTGTCTTTCGACTCTGCTTGTAGAAACCATTTGCACATATTCT
  GTACGAGCCTCGCTGTCTCCCTAGAGCCAGGGCCCTGCGGATTTGGAGAAGGGAGCGGGGCAGGACTTCC
  AGGAGGACCCCAACCCGGCCCGGAGAGGGAGGAGGAGGCCTCCAGGGGCGCGGAGCTCTGGGGATGGGCG
  TCGGGCCGGCAGTGGTGCGGCTCACTCCGTCCCTGCCCACCTGCGACGGGATCCCCCGACCGGCACGGGC
  CACGCCGAGCTCCCGGCCAGCCGCCGGCCCGCAGGCAGCGCGAGGGAGGAGCTGCGCCGCCGGGAAGGGG
  CCGCCGGCTCCGCCCAACCAGGTGGTGCTGAGCTTCCGCTGAGCGCTCTTTTGTTTTGTGGTTTGACACT
  TTTCTTGACAGCATGTTGCAGTTTCTTTTCGGTTTTGGTTTTTTTTAAATGTTTTATTTTGCTTTCCCAG
  CGGGAGGGGAGGAAGAAGAGTGTTTACAAAGTCCTGTAGCCCCCTCACCTTTCTGTTTTCACTTTTGCCA
  ATGTACATCGGGTTTGGTTTTCTTGTATTATTTAAACGGTTGTGGTTTCCTTTTTCCACGGAGGTTCAAT
  AGAAGCCGCTGCAGGAGAGTTTTACCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_199460
• Insert Size: 8000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_199460.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199460.2, NP_955630.2
• RefSeq Size: 13729 bp
• RefSeq ORF: 6666 bp
• Locus ID: 775
• Cytogenetics: 12p13.33
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Alzheimer's disease, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, GnRH signaling pathway, Hypertrophic cardiomyopathy (HCM), Long-term potentiation, MAPK signaling pathway, Type II diabetes mellitus, Vascular smooth muscle contraction
• Gene Summary: 'This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins. Some of the predicted proteins may not produce functional ion channel subunits. [provided by RefSeq, Oct 2012]'
  Transcript Variant: This variant (1), also referred to as HFCC, encodes the longest isoform (1). This isoform (1) is predicted to form a non-functional calcium channel subunit because it contains an aberrant number of transmembrane domains. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.