CXCR2 (NM_001168298) Human Untagged Clone

CAT#: SC328574

CXCR2 (untagged)-Human chemokine (C-X-C motif) receptor 2 (CXCR2) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CXCR2
• Synonyms: CD182; CDw128b; CMKAR2; IL8R2; IL8RA; IL8RB
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001557.2 CTTCAGACTGGTAGGCTCCTCCAGAAGCCATCAGACAGGAAGATGTGAAAATCCCCAGCA
  CTCATCCCAGAATCACTAAGTGGCACCTGTCCTGGGCCAAAGTCCCAGGACAGACCTCAT
  TGTTCCTCTGTGGGAATACCTCCCCAGGAGGGCATCCTGGATTTCCCCCTTGCAACCCAG
  GTCAGAAGTTTCATCGTCAAGGTTGTTTCATCTTTTTTTTCCTGTCTAACAGCTCTGACT
  ACCACCCAACCTTGAGGCACAGTGAAGACATCGGTGGCCACTCCAATAACAGCAGGTCAC
  AGCTGCTCTTCTGGAGGTGTCCTACAGGTGAAAAGCCCAGCGACCCAGTCAGGATTTAAG
  TTTACCTCAAAAATGGAAGATTTTAACATGGAGAGTGACAGCTTTGAAGATTTCTGGAAA
  GGTGAAGATCTTAGTAATTACAGTTACAGCTCTACCCTGCCCCCTTTTCTACTAGATGCC
  GCCCCATGTGAACCAGAATCCCTGGAAATCAACAAGTATTTTGTGGTCATTATCTATGCC
  CTGGTATTCCTGCTGAGCCTGCTGGGAAACTCCCTCGTGATGCTGGTCATCTTATACAGC
  AGGGTCGGCCGCTCCGTCACTGATGTCTACCTGCTGAACCTAGCCTTGGCCGACCTACTC
  TTTGCCCTGACCTTGCCCATCTGGGCCGCCTCCAAGGTGAATGGCTGGATTTTTGGCACA
  TTCCTGTGCAAGGTGGTCTCACTCCTGAAGGAAGTCAACTTCTATAGTGGCATCCTGCTA
  CTGGCCTGCATCAGTGTGGACCGTTACCTGGCCATTGTCCATGCCACACGCACACTGACC
  CAGAAGCGCTACTTGGTCAAATTCATATGTCTCAGCATCTGGGGTCTGTCCTTGCTCCTG
  GCCCTGCCTGTCTTACTTTTCCGAAGGACCGTCTACTCATCCAATGTTAGCCCAGCCTGC
  TATGAGGACATGGGCAACAATACAGCAAACTGGCGGATGCTGTTACGGATCCTGCCCCAG
  TCCTTTGGCTTCATCGTGCCACTGCTGATCATGCTGTTCTGCTACGGATTCACCCTGCGT
  ACGCTGTTTAAGGCCCACATGGGGCAGAAGCACCGGGCCATGCGGGTCATCTTTGCTGTC
  GTCCTCATCTTCCTGCTCTGCTGGCTGCCCTACAACCTGGTCCTGCTGGCAGACACCCTC
  ATGAGGACCCAGGTGATCCAGGAGACCTGTGAGCGCCGCAATCACATCGACCGGGCTCTG
  GATGCCACCGAGATTCTGGGCATCCTTCACAGCTGCCTCAACCCCCTCATCTACGCCTTC
  ATTGGCCAGAAGTTTCGCCATGGACTCCTCAAGATTCTAGCTATACATGGCTTGATCAGC
  AAGGACTCCCTGCCCAAAGACAGCAGGCCTTCCTTTGTTGGCTCTTCTTCAGGGCACACT
  TCCACTACTCTCTAAGACCTCCTGCCTAAGTGCAGCCCCGTGGGGTTCCTCCCTTCTCTT
  CACAGTCACATTCCAAGCCTCATGTCCACTGGTTCTTCTTGGTCTCAGTGTCAATGCAGC
  CCCCATTGTGGTCACAGGAAGTAGAGGAGGCCACGTTCTTACTAGTTTCCCTTGCATGGT
  TTAGAAAGCTTGCCCTGGTGCCTCACCCCTTGCCATAATTACTATGTCATTTGCTGGAGC
  TCTGCCCATCCTGCCCCTGAGCCCATGGCACTCTATGTTCTAAGAAGTGAAAATCTACAC
  TCCAGTGAGACAGCTCTGCATACTCATTAGGATGGCTAGTATCAAAAGAAAGAAAATCAG
  GCTGGCCAACGGGGTGAAACCCTGTCTCTACTAAAAATACAAAAAAAAAAAAAAATTAGC
  CGGGCGTGGTGGTGAGTGCCTGTAATCACAGCTACTTGGGAGGCTGAGATGGGAGAATCA
  CTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATTGTGCCCCTGCACTCCAGCCTG
  AGCGACAGTGAGACTCTGTCTCAGTCCATGAAGATGTAGAGGAGAAACTGGAACTCTCGA
  GCGTTGCTGGGGGGGATTGTAAAATGGTGTGACCACTGCAGAAGACAGTATGGCAGCTTT
  CCTCAAAACTTCAGACATAGAATTAACACATGATCCTGCAATTCCACTTATAGGAATTGA
  CCCACAAGAAATGAAAGCAGGGACTTGAACCCATATTTGTACACCAATATTCATAGCAGC
  TTATTCACAAGACCCAAAAGGCAGAAGCAACCCAAATGTTCATCAATGAATGAATGAATG
  GCTAAGCAAAATGTGATATGTACCTAACGAAGTATCCTTCAGCCTGAAAGAGGAATGAAG
  TACTCATACATGTTACAACACGGACGAACCTTGAAAACTTTATGCTAAGTGAAATAAGCC
  AGACATCAACAGATAAATAGTTTATGATTCCACCTACATGAGGTACTGAGAGTGAACAAA
  TTTACAGAGACAGAAAGCAGAACAGTGATTACCAGGGACTGAGGGGAGGGGAGCATGGGA
  AGTGACGGTTTAATGGGCACAGGGTTTATGTTTAGGATGTTGAAAAAGTTCTGCAGATAA
  ACAGTAGTGATAGTTGTACCGCAATGTGACTTAATGCCACTAAATTGACACTTAAAAATG
  GTTTAAATGGTCAATTTTGTTATGTATATTTTATATCAATTTAAAAAAAAACCTGAGCCC
  CAAAAGGTATTTTAATCACCAAGGCTGATTAAACCAAGGCTAGAACCACCTGCCTATATT
  TTTTGTTAAATGATTTCATTCAATATCTTTTTTTTAATAAACCATTTTTACTTGGGTGTT
  TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001168298
• Insert Size: 2900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168298.1, NP_001161770.1
• RefSeq Size: 2672 bp
• RefSeq ORF: 1083 bp
• Locus ID: 3579
• Cytogenetics: 2q35
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection
• Gene Summary: 'The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.