GCAT (NM_001171690) Human Untagged Clone

CAT#: SC328730

GCAT (untagged)-Human glycine C-acetyltransferase (GCAT) nuclear gene encoding mitochondrial protein transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCAT
• Synonyms: KBL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001171690, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGCCTGGGAACGCCTGGCGCGCCGCACTCTTCTGGGTGCCCCGCGGCCGCCGCGCACAGTCAGCGC
  TGGCCCAGCTGCGTGGCATTCTGGAGGGGGAGCTGGAAGGCATCCGCGGAGCTGGCACTTGGAAGAGTGA
  GCGGGTCATCACGTCCCGTCAGGGGCCGCACATCCGCGTGGACGGCGTCTCCGGAGGGCCTGGCACTGTC
  ATCTTTCCAGGCCTGCCCTTGCCCCACCTGAGCTGCTGTATCCATCTCCTCTCCTTCACCTCAGGAATCC
  TTAACTTCTGTGCCAACAACTACCTGGGCCTGAGCAGCCACCCTGAGGTGATCCAGGCAGGTCTGCAGGC
  TCTGGAGGAGTTTGGAGCTGGCCTCAGCTCTGTCCGCTTTATCTGTGGAACCCAGAGCATCCACAAGAAT
  CTAGAAGCAAAAATAGCCCGCTTCCACCAGCGGGAGGATGCCATCCTCTATCCCAGCTGTTATGACGCCA
  ACGCCGGCCTCTTTGAGGCCCTGCTGACCCCAGAGGACGCAGTCCTGTCGGACGAGCTGAACCATGCCTC
  CATCATCGACGGCATCCGGCTGTGCAAGGCCCACAAGTACCGCTATCGCCACCTGGACATGGCCGACCTA
  GAAGCCAAGCTGCAGGAGGCCCAGAAGCATCGGCTGCGCCTGGTGGCCACTGATGGGGCCTTTTCCATGG
  ATGGCGACATCGCACCCCTGCAGGAGATCTGCTGCCTCGCCTCTAGATATGGTGCCCTGGTCTTCATGGA
  TGAATGCCATGCCACTGGCTTCCTGGGGCCCACAGGACGGGGCACAGATGAGCTGCTGGGTGTGATGGAC
  CAGGTCACCATCATCAACTCCACCCTGGGGAAGGCCCTGGGTGGAGCATCAGGGGGCTACACGACAGGGC
  CTGGGCCCCTGGTGTCCCTGCTGCGGCAGCGCGCCCGGCCATACCTCTTCTCCAACAGTCTGCCACCTGC
  TGTCGTTGGCTGCGCCTCCAAGGCCCTAGATCTGCTGATGGGGAGTAACACCATTGTCCAGTCTATGGCT
  GCCAAGACCCAGAGGTTCCGTAGTAAGATGGAAGCTGCTGGCTTCACTATCTCGGGAGCCAGTCACCCCA
  TCTGCCCTGTGATGCTGGGTGATGCCCGGCTGGCCTCTCGCATGGCGGATGACATGCTGAAGAGAGGCAT
  CTTTGTCATCGGGTTCAGCTACCCCGTGGTCCCCAAGGGCAAGGCCCGGATCCGGGTACAGATCTCAGCA
  GTGCATAGCGAGGAAGACATTGACCGCTGCGTGGAGGCCTTCGTGGAAGTGGGGCGACTGCACGGGGCAC
  TGCCCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001171690
• ORF Size: 1338 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001171690.1, NP_001165161.1
• RefSeq Size: 1656
• RefSeq ORF: 1338
• Locus ID: 23464
• Protein Pathways: Glycine, serine and threonine metabolism
• Gene Summary: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).