PCCB (NM_001178014) Human Untagged Clone

CAT#: SC328913

PCCB (untagged)-Human propionyl CoA carboxylase beta polypeptide (PCCB) nuclear gene encoding mitochondrial protein transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCCB
• Synonyms: DKFZp451E113
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001178014, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGCATTACGGGTGGCGGCGGTCGGGGCAAGGCTCAGCGTTCTGGCGAGCGGTCTCCGCGCCG
  CGGTCCGCAGCCTTTGCAGCCAGGCCACCTCTGTTAACGAACGCATCGAAAACAAGCGCCGGACCGCGCT
  GCTGGGAGGGGGCCAACGCCGTATTGACGCGCAGCACAAGCGAGGAAAGCTAACAGCCAGGGAGAGGATC
  AGTCTCTTGCTGGACCCTGGCAGCTTTGTTGAGAGCGACATGTTTGTGGAACACAGATGTGCAGATTTTG
  GAATGGCTGCTGATAAGAATAAGTTTCCTGGAGACAGCGTGGTCACTGGACGAGGCCGAATCAATGGAAG
  ATTGGTTTATGTCTTCAGTCAGCAAATAATAGGCTGGGCACAGTGGCTCCCGCTTGTCATCTCAGCACTT
  TGGGAGGCCGAGGATTTTACAGTTTTTGGAGGCAGTCTGTCAGGAGCACATGCCCAAAAGATCTGCAAAA
  TCATGGACCAGGCCATAACGGTGGGGGCTCCAGTGATTGGGCTGAATGACTCTGGGGGAGCACGGATCCA
  AGAAGGAGTGGAGTCTTTGGCTGGCTATGCAGACATCTTTCTGAGGAATGTTACGGCATCCGGAGTCATC
  CCTCAGATTTCTCTGATCATGGGCCCATGTGCTGGTGGGGCCGTCTACTCCCCAGCCCTAACAGACTTCA
  CGTTCATGGTAAAGGACACCTCCTACCTGTTCATCACTGGCCCTGATGTTGTGAAGTCTGTCACCAATGA
  GGATGTTACCCAGGAGGAGCTCGGTGGTGCCAAGACCCACACCACCATGTCAGGTGTGGCCCACAGAGCT
  TTTGAAAATGATGTTGATGCCTTGTGTAATCTCCGGGATTTCTTCAACTACCTGCCCCTGAGCAGTCAGG
  ACCCGGCTCCCGTCCGTGAGTGCCACGATCCCAGTGACCGTCTGGTTCCTGAGCTTGACACAATTGTCCC
  TTTGGAATCAACCAAAGCCTACAACATGGTGGACATCATACACTCTGTTGTTGATGAGCGTGAATTTTTT
  GAGATCATGCCCAATTATGCCAAGAACATCATTGTTGGTTTTGCAAGAATGAATGGGAGGACTGTTGGAA
  TTGTTGGCAACCAACCTAAGGTGGCCTCAGGATGCTTGGATATTAATTCATCTGTGAAAGGGGCTCGTTT
  TGTCAGATTCTGTGATGCATTCAATATTCCACTCATCACTTTTGTTGATGTCCCTGGCTTTCTACCTGGC
  ACAGCACAGGAATACGGGGGCATCATCCGGCATGGTGCCAAGCTTCTCTACGCATTTGCTGAGGCAACTG
  TACCCAAAGTCACAGTCATCACCAGGAAGGCCTATGGAGGTGCCTATGATGTCATGAGCTCTAAGCACCT
  TTGTGGTGATACCAACTATGCCTGGCCCACCGCAGAGATTGCAGTCATGGGAGCAAAGGGCGCTGTGGAG
  ATCATCTTCAAAGGGCATGAGAATGTGGAAGCTGCTCAGGCAGAGTACATCGAGAAGTTTGCCAACCCTT
  TCCCTGCAGCAGTGCGAGGGTTTGTGGATGACATCATCCAACCTTCTTCCACACGTGCCCGAATCTGCTG
  TGACCTGGATGTCTTGGCCAGCAAGAAGGTACAACGTCCTTGGAGAAAACATGCAAATATTCCATTGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001178014
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178014.1, NP_001171485.1
• RefSeq Size: 1885 bp
• RefSeq ORF: 1680 bp
• Locus ID: 5096
• Cytogenetics: 3q22.3
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: 'The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]'
  Transcript Variant: This variant (2) has an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is longer compared to isoform 1.