DDX4 (NM_001166534) Human Untagged Clone

CAT#: SC328935

DDX4 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4) transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX4
• Synonyms: VASA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001166534, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGTCTAGGAATTTATTTTTAACCAACTCTCCAGAGTCTAGTAATGACTGCGAAGATAATCCAACAC
  GGAACAGAGGGTTTTCCAAGAGAGGCGATAATGACTTAGACCCAGACGAATGTATGCAGCGCACTGGTGG
  CCTTTTTGGTTCTAGAAGACCAGTATTAAGTGGCACAGGTAATGGTGATACTTCTCAAAGCAGAAGTGGC
  AGTGGAAGTGAACGAGATTCTTGGAAGTCAGAAGCAGAAGGAGGAGAAAGTAGTGATACTCAAGGACCAA
  AAGTGACCTACATACCCCCTCCTCCACCTGAGGATGAGGACTCCATCTTTGCACATTATCAGACAGGCAT
  AAACTTCGACAAATACGACACTATTCTTGTGGAAGTGTCTGGACATGATGCACCACCAGCAATTCTGACT
  TTTGAAGAAGCTAATCTCTGTCAGACACTGAATAACAACATTGCTAAAGCTGGTTATACTAAGCTTACTC
  CTGTGCAAAAATACAGTATTCCTATCATACTTGCAGGACGAGATTTGATGGCTTGCGCTCAAACAGGGTC
  TGGGAAGACTGCGGCTTTTCTCCTACCAATTTTGGCTCATATGATGCATGATGGAATAACTGCCAGTCGT
  TTTAAAGAGTTGCAGGAACCAGAGTGTATTATTGTAGCACCAACTCGAGAATTGGTCAACCAGATTTATT
  TGGAAGCCAGAAAATTTTCTTTTGGGACTTGTGTAAGAGCTGTTGTTATATATGGGGGAACCCAGCTGGG
  ACATTCAATTCGACAAATAGTACAAGGCTGTAATATATTATGTGCTACTCCTGGAAGACTGATGGATATC
  ATAGGCAAAGAAAAGATTGGTCTCAAACAGATCAAATACTTAGTTTTGGATGAAGCTGATCGCATGTTGG
  ATATGGGTTTTGGTCCAGAAATGAAGAAGTTAATTTCTTGCCCAGGAATGCCATCAAAGGAACAGCGCCA
  AACCCTTATGTTCAGTGCAACTTTTCCAGAGGAAATTCAAAGGTTGGCTGCAGAGTTTTTAAAGTCAAAT
  TATCTGTTTGTTGCTGTTGGACAAGTGGGTGGAGCATGTAGAGATGTTCAGCAGACCGTTCTCCAAGTTG
  GCCAGTTCTCAAAAAGAGAAAAGCTCGTTGAAATTCTGCGAAACATAGGGGATGAAAGAACTATGGTCTT
  TGTTGAAACTAAGAAAAAAGCAGATTTTATTGCAACTTTTCTTTGTCAAGAAAAAATATCAACTACAAGT
  ATTCATGGTGATCGGGAACAGAGAGAGCGGGAGCAAGCTCTTGGAGATTTTCGCTTTGGAAAGTGCCCAG
  TTCTTGTTGCTACTTCAGTAGCTGCCAGAGGGCTGGATATTGAAAATGTGCAACATGTTATCAATTTTGA
  TCTTCCTTCTACCATTGATGAATATGTTCATCGAATTGGGCGTACTGGTCGTTGTGGGAATACTGGCAGA
  GCAATTTCCTTTTTTGATCTTGAATCGGATAACCATTTAGCACAGCCTCTAGTAAAAGTATTGACAGATG
  CTCAACAGGATGTTCCTGCATGGTTGGAAGAAATTGCCTTTAGTACATACATTCCTGGCTTCAGTGGTAG
  TACAAGAGGAAACGTGTTTGCATCAGTTGATACCAGAAAGGGCAAGAGCACTTTGAACACAGCTGGGTTT
  TCTTCTTCACAAGCTCCCAATCCAGTAGATGATGAGTCATGGGATTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001166534
• ORF Size: 1728 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001166534.1, NP_001160006.1
• RefSeq Size: 2379
• RefSeq ORF: 1728
• Locus ID: 54514
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (4) has a novel first exon representing use of an alternate internal promoter site compared to variant 1. The predicted protein isoform (4) is shorter and has a distinct N-terminus.