Phospholipase C beta 3 (PLCB3) (NM_001184883) Human Untagged Clone

CAT#: SC329306

PLCB3 (untagged)-Human phospholipase C beta 3 (phosphatidylinositol-specific) (PLCB3) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLCB3
• Synonyms: FLJ37084
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001184883, the custom clone sequence may differ by one or more nucleotides
  ATGGCGGGCGCCCAGCCCGGCGTCCACGCGCTGCAGTTGGAGCCGCCCACCGTGGTGGAG
  ACCCTGCGGCGCGGGAGTAAGTTCATCAAATGGGACGAGGAGAAGCTGATGACGGTGGTG
  TCTGGGCCAGACCCAGTGAACACAGTGTTCTTGAACTTCATGGCCGTGCAGGATGACACA
  GCCAAGGTCTGGTCTGAGGAGCTATTCAAGCTGGCTATGAACATCCTGGCTCAGAACGCC
  TCCCGGAACACCTTCCTGCGCAAAGCATACACGAAGCTGAAGCTGCAGGTGAACCAGGAT
  GGTCGGATCCCCGTCAAGAACATCCTGAAGATGTTCTCAGCAGACAAGAAGCGGGTGGAG
  ACTGCGCTGGAATCCTGTGGCCTCAAATTCAACCGGAGTGAGTCCATCCGGCCTGATGAG
  TTTTCCTTGGAAATCTTTGAGCGGTTCCTGAACAAGCTGTGTCTGCGGCCGGACATTGAC
  AAGATCCTGCTGGAGATAGGCGCCAAGGGCAAGCCATACCTGACGCTGGAGCAGCTCATG
  GACTTCATCAACCAGAAGCAACGCGACCCGAGACTCAACGAAGTGCTGTACCCGCCCCTG
  CGGCCCTCCCAGGCCCGGCTGCTCATCGAAAAGTATGAGCCCAACCAGCAGTTTCTGGAG
  CGAGACCAGATGTCCATGGAGGGCTTTAGCCGCTACCTGGGAGGCGAGGAGAATGGCATC
  CTGCCCCTGGAAGCCCTGGATCTGAGCACGGACATGACCCAGCCACTGAGTGCCTACTTC
  ATCAACTCCTCGCATAACACCTATCTCACTGCGGGGCAGCTGGCTGGGACCTCGTCGGTG
  GAGATGTACCGCCAGGCACTACTATGGGGCTGCCGCTGCGTGGAGCTGGACGTGTGGAAG
  GGACGGCCGCCTGAGGAGGAACCCTTCATTACCCACGGCTTCACCATGACCACAGAGGTG
  CCTCTGCGCGACGTGCTGGAGGCCATTGCCGAGACTGCCTTCAAGACCTCGCCCTACCCC
  GTCATCCTCTCCTTCGAGAACCATGTGGACTCGGCAAAGCAACAGGCAAAGATGGCTGAG
  TACTGCCGCTCCATCTTTGGAGACGCGCTACTCATCGAGCCTCTGGACAAGTACCCGCTG
  GCCCCAGGCGTTCCCCTGCCCAGCCCCCAGGACCTGATGGGCCGTATCCTGGTGAAGAAC
  AAGAAGCGGCACCGACCCAGCGCAGGTGGCCCAGACAGCGCCGGGCGCAAGCGGCCCCTG
  GAGCAGAGCAATTCTGCCCTGAGCGAGAGCTCCGCGGCCACCGAGCCCTCCTCCCCGCAG
  CTGGGGTCTCCCAGCTCTGACAGCTGCCCAGGCCTGAGCAATGGGGAGGAGGTAGGGCTT
  GAGAAGCCCAGCCTGGAGCCTCAGAAGTCTCTGGGTGACGAGGGCCTGAACCGAGGCCCC
  TATGTTCTTGGACCTGCTGACCGTGAGGATGAGGAGGAAGATGAGGAAGAGGAGGAACAG
  ACAGACCCCAAAAAGCCAACTACAGATGAGGGCACAGCCAGCAGCGAGGTGAATGCCACT
  GAGGAGATGTCCACGCTTGTCAACTACATCGAACCTGTCAAGTTCAAGTCCTTTGAGGCT
  GCTCGAAAGAGGAACAAATGCTTCGAGATGTCGTCCTTTGTGGAGACCAAGGCCATGGAG
  CAACTGACCAAGAGCCCCATGGAGTTTGTGGAATACAACAAGCAGCAGCTCAGCCGCATC
  TACCCCAAGGGCACCCGCGTGGACTCCTCCAACTACATGCCCCAGCTCTTCTGGAACGTA
  GGGTGCCAGCTTGTTGCGCTCAACTTCCAGACCCTCGATGTGGCGATGCAGCTCAACGCG
  GGCGTTTTTGAGTACAACGGGCGCAGCGGGTACCTGCTCAAGCCGGAGTTCATGCGGCGG
  CCGGACAAGTCCTTCGACCCCTTCACTGAGGTCATCGTGGATGGCATCGTGGCCAATGCC
  TTGCGGGTCAAGGTGATCTCAGGGCAGTTCCTGTCCGACAGGAAGGTGGGCATCTACGTG
  GAGGTGGACATGTTTGGCCTCCCTGTTGATACGCGGCGCAAGTACCGCACCCGGACCTCT
  CAGGGGAACTCGTTCAACCCCGTGTGGGACGAAGAGCCCTTCGACTTCCCCAAGGTGGTG
  CTGCCCACGCTGGCTTCACTTCGCATTGCAGCCTTTGAGGAGGGGGGTAAATTCGTAGGG
  CACCGGATCCTGCCTGTCTCTGCCATCCGCTCCGGATACCACTACGTCTGCCTGCGGAAC
  GAGGCCAACCAACCGCTGTGCCTGCCGGCCCTGCTCATCTACACCGAAGCCTCGGACTAC
  ATTCCTGACGACCACCAGGACTATGCGGAGGCCCTGATCAACCCCATTAAGCACGTCAGC
  CTGATGGACCAGAGGGCCCGGCAGCTGGCCGCCCTCATTGGGGAGAGTGAGGCTCAGGCT
  GGCCAAGAGACGTGCCAGGACACCCAGTCTCAGCAGCTGGGGTCTCAGCCGTCCTCAAAC
  CCCACCCCCAGCCCACTGGATGCCTCCCCCCGCCGGCCCCCTGGCCCCACCACCTCCCCT
  GCCAGCACCTCCCTCAGCAGCCCAGGGCAGCGTGATGATCTCATCGCCAGCATCCTCTCA
  GAGGTGGCCCCCACCCCGCTGGATGAGCTCCGAGGTCACAAGGCTCTGGTCAAGCTCCGG
  AGCCGGCAAGAGCGAGACCTGCGGGAGCTGCGCAAGAAGCATCAGCGGAAGGCAGTCACC
  CTCACCCGCCGCCTGCTGGATGGCCTGGCTCAGGCACAGGCTGAGGGCAGGTGCCGGCTG
  CGGCCAGGTGCCCTAGGTGGGGCCGCTGATGTGGAGGACACGAAGGAGGGGGAGGACGAG
  GCAAAGCGGTATCAGGAGTTCCAGAACAGACAGGTGCAGAGCCTGCTGGAGCTGCGGGAG
  GCCCAGGTGGACGCAGAGGCCCAGCGGAGGCTGGAACACCTGAGACAGGCTCTGCAGCGG
  CTCAGGGAGGTCGTCCTTGATGCAAACACAACTCAGTTCAAGAGGCTGAAAGAGATGAAC
  GAGAGGGAGAAGAAGGAGCTGCAGAAGATCCTGGACAGAAAGCGCCATAACAGCATCTCG
  GAGGCCAAGATGAGGGACAAGCATAAGAAGGAGGCGGAACTGACGGAGATTAACCGTCGG
  CACATCACTGAGTCAGTCAACTCCATCCGTCGGCTGGAGGAGGCCCAGAAGCAGCGGCAT
  GACCGTCTTGTGGCTGGGCAGCAGCAGGTCCTGCAACAGCTGGCAGAAGAGGAGCCCAAG
  CTGCTGGCCCAGCTGGCCCAGGAGTGTCAGGAGCAGCGGGCGAGGCTCCCCCAGGAGATC
  CGCCGGAGCCTGCTGGGCGAGATGCCGGAGGGGCTGGGGGACGGGCCTCTGGTGGCCTGT
  GCCAGCAACGGTCACGCACCCGGGAGCAGCGGGCACCTGTCGGGCGCTGACTCGGAGAGC
  CAGGAGGAGAACACGCAGCTCTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001184883
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184883.1, NP_001171812.1
• RefSeq Size: 5527 bp
• RefSeq ORF: 3504 bp
• Locus ID: 5331
• Cytogenetics: 11q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway, Chemokine signaling pathway, Gap junction, GnRH signaling pathway, Huntington's disease, Inositol phosphate metabolism, Long-term depression, Long-term potentiation, Melanogenesis, Metabolic pathways, Phosphatidylinositol signaling system, Vascular smooth muscle contraction, Wnt signaling pathway
• Gene Summary: 'This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]'
  Transcript Variant: This variant (2) lacks an in-frame portion of the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.