NDUFS1 (NM_001199984) Human Untagged Clone

CAT#: SC329608

NDUFS1 (untagged) - Homo sapiens NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) (NDUFS1), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFS1
• Synonyms: CI-75k; CI-75Kd; MC1DN5; PRO1304
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001199984, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGATCAGGGGGTCCTCGGGAACGCTGTCACGAATCAATATGTTAAGGATACCTGTAAGAAAGGCCT
  TAGTAGGCCTTTCTAAGTCTCCTAAAGGATGTGTTCGAACAACTGCCACAGCAGCAAGCAACTTGATTGA
  AGTATTTGTTGATGGTCAGTCTGTCATGGTGGAACCGGGAACGACCGTCCTCCAAGCTTGTGAGAAGGTT
  GGCATGCAGATCCCTCGATTCTGTTATCATGAAAGGTTGTCTGTTGCTGGAAACTGCAGGATGTGCCTTG
  TTGAAATTGAGAAAGCCCCTAAGGTTGTAGCTGCTTGTGCCATGCCAGTAATGAAGGGTTGGAATATCCT
  AACAAACTCAGAAAAATCCAAAAAAGCCAGGGAAGGTGTGATGGAGTTCTTATTAGCAAATCACCCATTG
  GACTGTCCTATTTGTGACCAGGGAGGTGAATGTGATCTGCAGGACCAGTCCATGATGTTTGGAAATGATA
  GGAGCCGATTTTTAGAGGGGAAGCGTGCTGTGGAAGACAAGAACATTGGGCCATTGGTAAAGACCATCAT
  GACAAGATGTATACAGTGTACTCGCTGCATCAGGTTTGCAAGTGAGATTGCAGGAGTAGATGATTTGGGA
  ACAACAGGCAGAGGAAATGATATGCAAGTTGGCACATACATTGAAAAGATGTTCATGTCTGAACTGTCTG
  GGAATATCATTGATATCTGCCCTGTAGGTGCCCTAACCTCTAAGCCCTATGCCTTTACTGCCCGGCCTTG
  GGAAACAAGAAAGACAGAATCCATTGATGTAATGGATGCGGTTGGAAGTAATATTGTGGTTAGCACAAGA
  ACTGGAGAAGTGATGAGGATTTTGCCACGTATGCATGAGGACATCAATGAAGAGTGGATCTCTGATAAAA
  CCAGATTTGCCTATGATGGGCTAAAACGTCAAAGACTTACCGAGCCAATGGTCAGAAATGAAAAAGGGCT
  TTTAACCTATACTTCTTGGGAGGATGCGCTCTCTCGCGTAGCTGGAATGTTGCAGAGTTTTCAAGGCAAA
  GATGTGGCAGCAATTGCAGGTGGCTTGGTGGATGCTGAAGCCCTGGTAGCTCTCAAAGATTTGCTTAATA
  GAGTGGACTCTGACACCTTATGCACTGAAGAGGTCTTCCCCACTGCAGGAGCTGGCACAGATTTGCGTTC
  CAATTATCTTCTTAATACTACAATTGCTGGTGTGGAAGAGGCAGATGTTGTTCTTCTGGTTGGTACAAAC
  CCACGTTTTGAGGCACCACTGTTTAATGCTAGAATTCGAAAGAGCTGGCTGCATAATGACTTAAAAGTGG
  CCCTTATAGGCAGTCCAGTGGACCTCACTTACACATATGACCACCTGGGAGACTCCCCCAAAATTCTTCA
  AGACATTGCTTCGGGAAGCCATCCATTTAGCCAGGTCCTAAAGGAAGCTAAAAAACCAATGGTGGTTTTA
  GGCAGTTCTGCACTCCAAAGAAATGATGGAGCAGCAATTCTTGCAGCTGTTTCTAGCATTGCACAAAAGA
  TTCGGATGACTAGTGGTGTTACTGGTGATTGGAAAGTTATGAATATCCTTCATAGGATTGCAAGTCAAGT
  AGCTGCTTTGGACCTTGGCTATAAGCCTGGGGTGGAAGCAATTCGGAAGAACCCTCCCAAGGTGCTGTTT
  CTCCTGGGAGCAGATGGAGGTTGTATCACACGACAGGATTTGCCAAAGGATTGTTTCATTATTTATCAAG
  GACATCATGGTGATGTTGGGGCTCCCATAGCTGATGTTATTCTCCCAGGAGCTGCTTACACAGAGAAGTC
  TGCTACATATGTCAACACTGAGGGTAGAGCTCAGCAGACTAAGGTAGCAGTGACACCTCCTGGCTTGGCA
  AGAGAAGACTGGAAAATTATAAGAGCACTCTCTGAGATTGCTGGAATGACTCTTCCATATGATACTCTGG
  ATCAAGTAAGGAACAGATTGGAAGAAGTCTCTCCTAATCTTGTTCGATATGATGATATTGAAGGGGCTAA
  TTACTTCCAGCAAGCAAATGAGCTCTCAAAGCTAGTGAACCAGCAGCTTCTTGCTGACCCACTTGTTCCA
  CCTCAGCTAACTATAAAAGACTTCTACATGACAGATTCAATTAGCAGAGCCTCACAGACAATGGCCAAAT
  GTGTCAAAGCTGTCACAGAGGGTGCCCAGGCAGTAGAGGAACCATCCATATGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199984
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199984.1, NP_001186913.1
• RefSeq Size: 3393 bp
• RefSeq ORF: 2226 bp
• Locus ID: 4719
• Cytogenetics: 2q33.3
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]'
  Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (5) has a longer and distinct N-terminus compared to isoform 1.