NOS1 (NM_001204214) Human Untagged Clone

CAT#: SC329731

NOS1 (untagged) - Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NOS1
• Synonyms: bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001204214, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCTCCATCATGCATCCTTCTCAGCATGCAAGGAGGCCTGAAGACGTCCGCACAAAAGGACAGCTCT
  TCCCTCTCGCCAAAGAGTTTATTGATCAATACTATTCATCAATTAAAAGATTTGGCTCCAAAGCCCACAT
  GGAAAGGCTGGAAGAGGTGAACAAAGAGATCGACACCACTAGCACTTACCAGCTCAAGGACACAGAGCTC
  ATCTATGGGGCCAAGCACGCCTGGCGGAATGCCTCGCGCTGTGTGGGCAGGATCCAGTGGTCCAAGCTGC
  AGGTATTCGATGCCCGTGACTGCACCACGGCCCACGGGATGTTCAACTACATCTGTAACCATGTCAAGTA
  TGCCACCAACAAAGGGAACCTCAGGTCTGCCATCACCATATTCCCCCAGAGGACAGACGGCAAGCACGAC
  TTCCGAGTCTGGAACTCCCAGCTCATCCGCTACGCTGGCTACAAGCAGCCTGACGGCTCCACCCTGGGGG
  ACCCAGCCAATGTGCAGTTCACAGAGATATGCATACAGCAGGGCTGGAAACCGCCTAGAGGCCGCTTCGA
  TGTCCTGCCGCTCCTGCTTCAGGCCAACGGCAATGACCCTGAGCTCTTCCAGATTCCTCCAGAGCTGGTG
  TTGGAAGTTCCCATCAGGCACCCCAAGTTTGAGTGGTTCAAGGACCTGGGGCTGAAGTGGTACGGCCTCC
  CCGCCGTGTCCAACATGCTCCTAGAGATTGGCGGCCTGGAGTTCAGCGCCTGTCCCTTCAGTGGCTGGTA
  CATGGGCACAGAGATTGGTGTCCGCGACTACTGTGACAACTCCCGCTACAATATCCTGGAGGAAGTGGCC
  AAGAAGATGAACTTAGACATGAGGAAGACGTCCTCCCTGTGGAAGGACCAGGCGCTGGTGGAGATCAATA
  TCGCGGTTCTCTATAGCTTCCAGAGTGACAAAGTGACCATTGTTGACCATCACTCCGCCACCGAGTCCTT
  CATTAAGCACATGGAGAATGAGTACCGCTGCCGGGGGGGCTGCCCTGCCGACTGGGTGTGGATCGTGCCC
  CCCATGTCCGGAAGCATCACCCCTGTGTTCCACCAGGAGATGCTCAACTACCGGCTCACCCCCTCCTTCG
  AATACCAGCCTGATCCCTGGAACACGCATGTCTGGAAAGGCACCAACGGGACCCCCACAAAGCGGCGAGC
  CATTGGCTTCAAGAAGCTAGCAGAAGCTGTCAAGTTCTCGGCCAAGCTGATGGGGCAGGCTATGGCCAAG
  AGGGTGAAAGCGACCATCCTCTATGCCACAGAGACAGGCAAATCGCAAGCTTATGCCAAGACCTTGTGTG
  AGATCTTCAAACACGCCTTTGATGCCAAGGTGATGTCCATGGAAGAATATGACATTGTGCACCTGGAACA
  TGAAACTCTGGTCCTTGTGGTCACCAGCACCTTTGGCAATGGAGATCCCCCTGAGAATGGGGAGAAATTC
  GGCTGTGCTTTGATGGAAATGAGGCACCCCAACTCTGTGCAGGAAGAAAGGAAGAGCTACAAGGTCCGAT
  TCAACAGCGTCTCCTCCTACTCTGACTCCCAAAAATCATCAGGCGATGGGCCCGACCTCAGAGACAACTT
  TGAGAGTGCTGGACCCCTGGCCAATGTGAGGTTCTCAGTTTTTGGCCTCGGCTCACGAGCATACCCTCAC
  TTTTGCGCCTTCGGACACGCTGTGGACACCCTCCTGGAAGAACTGGGAGGGGAGAGGATCCTGAAGATGA
  GGGAAGGGGATGAGCTCTGTGGGCAGGAAGAGGCTTTCAGGACCTGGGCCAAGAAGGTCTTCAAGGCAGC
  CTGTGATGTCTTCTGTGTGGGAGATGATGTCAACATTGAAAAGGCCAACAATTCCCTCATCAGCAATGAT
  CGCAGCTGGAAGAGAAACAAGTTCCGCCTCACCTTTGTGGCCGAAGCTCCAGAACTCACACAAGGTCTAT
  CCAATGTCCACAAAAAGCGAGTCTCAGCTGCCCGGCTCCTTAGCCGTCAAAACCTCCAGAGCCCTAAATC
  CAGTCGGTCAACTATCTTCGTGCGTCTCCACACCAACGGGAGCCAGGAGCTGCAGTACCAGCCTGGGGAC
  CACCTGGGTGTCTTCCCTGGCAACCACGAGGACCTCGTGAATGCCCTGATCGAGCGGCTGGAGGACGCGC
  CGCCTGTCAACCAGATGGTGAAAGTGGAACTGCTGGAGGAGCGGAACACGGCTTTAGGTGTCATCAGTAA
  CTGGACAGACGAGCTCCGCCTCCCGCCCTGCACCATCTTCCAGGCCTTCAAGTACTACCTGGACATCACC
  ACGCCACCAACGCCTCTGCAGCTGCAGCAGTTTGCCTCCCTAGCTACCAGCGAGAAGGAGAAGCAGCGTC
  TGCTGGTCCTCAGCAAGGGTTTGCAGGAGTACGAGGAATGGAAATGGGGCAAGAACCCCACCATCGTGGA
  GGTGCTGGAGGAGTTCCCATCTATCCAGATGCCGGCCACCCTGCTCCTGACCCAGCTGTCCCTGCTGCAG
  CCCCGCTACTATTCCATCAGCTCCTCCCCAGACATGTACCCTGATGAAGTGCACCTCACTGTGGCCATCG
  TTTCCTACCGCACTCGAGATGGAGAAGGACCAATTCACCACGGCGTATGCTCCTCCTGGCTCAACCGGAT
  ACAGGCTGACGAACTGGTCCCCTGTTTCGTGAGAGGAGCACCCAGCTTCCACCTGCCCCGGAACCCCCAA
  GTCCCCTGCATCCTCGTTGGACCAGGCACCGGCATTGCCCCTTTCCGAAGCTTCTGGCAACAGCGGCAAT
  TTGATATCCAACACAAAGGAATGAACCCCTGCCCCATGGTCCTGGTCTTCGGGTGCCGGCAATCCAAGAT
  AGATCATATCTACAGGGAAGAGACCCTGCAGGCCAAGAACAAGGGGGTCTTCAGAGAGCTGTACACGGCT
  TACTCCCGGGAGCCAGACAAACCAAAGAAGTACGTGCAGGACATCCTGCAGGAGCAGCTGGCGGAGTCTG
  TGTACCGAGCCCTGAAGGAGCAAGGGGGCCACATATACGTCTGTGGGGACGTCACCATGGCTGCTGATGT
  CCTCAAAGCCATCCAGCGCATCATGACCCAGCAGGGGAAGCTCTCGGCAGAGGACGCCGGCGTATTCATC
  AGCCGGATGAGGGATGACAACCGATACCATGAGGATATTTTTGGAGTCACCCTGCGAACGTACGAAGTGA
  CCAACCGCCTTAGATCTGAGTCCATTGCCTTCATTGAAGAGAGCAAAAAAGACACCGATGAGGTTTTCAG
  CTCCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001204214
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204214.1, NP_001191143.1
• RefSeq Size: 10776 bp
• RefSeq ORF: 3297 bp
• Locus ID: 4842
• Cytogenetics: 12q24.22
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer
• Gene Summary: 'The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (4, also known as TnNOSb) contains 2 unique alternate exons (Tex 1b and Tex 2) at the 5' end compared to variant 1, resulting in translation initiation from a downstream AUG, and an isoform (3, also known as nNOSgamma) with a shorter N-terminus compared to isoform 1. This variant is specifically expressed in the testis, and the encoded isoform has catalytic activity (PMID:9111048). Variants 3 and 4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.