RUNX1T1 (NM_001198625) Human Untagged Clone

CAT#: SC331210

RUNX1T1 (untagged) - Homo sapiens runt-related transcription factor 1, translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RUNX1T1
• Synonyms: AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001198625, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTGATCGTACTGAGAAGCACTCCACAATGCCAGACTCACCTGTGGATGTGAAGACGCAATCTAGGC
  TGACTCCTCCAACAATGCCACCTCCCCCAACTACTCAAGGAGCTCCAAGAACCAGTTCATTTACACCGAC
  AACGTTAACTAATGGCACGAGCCATTCTCCTACAGCCTTGAATGGCGCCCCCTCACCACCCAATGGCTTC
  AGCAATGGGCCTTCCTCTTCTTCCTCCTCCTCTCTGGCTAATCAACAGCTGCCCCCAGCCTGTGGTGCCA
  GGCAACTCAGCAAGCTGAAAAGGTTCCTTACTACCCTGCAGCAGTTTGGCAATGACATTTCACCCGAGAT
  AGGAGAAAGAGTTCGCACCCTCGTTCTGGGACTAGTGAACTCCACTTTGACAATTGAAGAATTTCATTCC
  AAACTGCAAGAAGCTACTAACTTCCCACTGAGACCTTTTGTCATCCCATTTTTGAAGGCCAACTTGCCCC
  TGCTGCAGCGTGAGCTCCTCCACTGCGCAAGACTGGCCAAACAGAACCCTGCCCAGTACCTCGCCCAGCA
  TGAACAGCTGCTTCTGGATGCCAGCACCACCTCACCTGTTGACTCCTCAGAGCTGCTTCTCGATGTGAAC
  GAAAACGGGAAGAGGCGAACTCCAGACAGAACCAAAGAAAATGGCTTTGACAGAGAGCCTTTGCACTCAG
  AACATCCAAGCAAGCGACCATGCACTATTAGCCCAGGCCAGCGGTACAGTCCAAATAACGGCTTATCCTA
  CCAGCCCAATGGCCTGCCTCACCCTACCCCACCTCCACCTCAGCATTACCGTTTGGATGATATGGCCATT
  GCCCACCACTACAGGGACTCCTATCGACACCCCAGCCACAGGGACCTCAGGGACAGAAACAGACCTATGG
  GGTTGCATGGCACACGTCAAGAAGAAATGATTGATCACAGACTAACAGACAGAGAATGGGCAGAAGAGTG
  GAAACATCTTGACCATCTGTTAAACTGCATAATGGACATGGTAGAAAAAACAAGGCGATCTCTCACCGTA
  CTAAGGCGGTGTCAAGAAGCAGACCGGGAAGAATTGAATTACTGGATCCGGCGGTACAGTGACGCCGAGG
  ACTTAAAAAAAGGTGGCGGCAGTAGCAGCAGCCACTCTAGGCAGCAGAGTCCCGTCAACCCAGACCCAGT
  TGCACTAGACGCGCATCGGGAATTCCTTCACAGGCCTGCGTCTGGATACGTGCCAGAGGAGATCTGGAAG
  AAAGCTGAGGAGGCCGTCAATGAGGTGAAGCGCCAGGCGATGACGGAGCTGCAGAAGGCCGTGTCTGAGG
  CGGAGCGGAAAGCCCACGACATGATCACAACAGAGAGGGCCAAGATGGAGCGCACGGTCGCCGAGGCCAA
  ACGGCAGGCGGCGGAGGACGCACTGGCAGTTATCAATCAGCAGGAGGATTCAAGCGAGAGTTGCTGGAAT
  TGTGGCCGTAAAGCGAGTGAAACCTGCAGTGGCTGTAACACAGCCCGATACTGTGGCTCATTTTGCCAGC
  ACAAAGACTGGGAGAAGCACCATCACATCTGTGGACAGACCCTGCAGGCCCAGCAGCAGGGAGACACACC
  TGCAGTCAGCTCCTCTGTCACGCCCAACAGCGGGGCTGGGAGCCCGATGGACACACCACCAGCAGCCACT
  CCGAGGTCAACCACCCCGGGAACCCCTTCCACCATAGAGACAACCCCTCGCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198625
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198625.1, NP_001185554.1
• RefSeq Size: 7769 bp
• RefSeq ORF: 1734 bp
• Locus ID: 862
• Cytogenetics: 8q21.3
• Protein Families: Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer
• Gene Summary: 'This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]'
  Transcript Variant: This variant (5) utilizes four alternate exons in the 5' UTR compared to variant 1. Variants 1, 5 and 6 all encode isoform A, also known as MTG8a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.