Corticotropin Releasing Factor Receptor 2 (CRHR2) (NM_001202475) Human Untagged Clone

CAT#: SC331458

CRHR2 (untagged) - Homo sapiens corticotropin releasing hormone receptor 2 (CRHR2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CRHR2
• Synonyms: CRF-RB; CRF2; CRFR2; HM-CRF
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001202475, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGGGTCCCTCAGGGCCCCCAGGCCTCCTCTACGTCCCACACCTCCTCCTCTGCCTGCTCTGCCTCC
  TCCCACCGCCGCTCCAATACGCAGCCGGGCAGAGCCAGATGCCCAAAGACCAGCCCCTGTGGGCACTTCT
  GGAGCAGTACTGCCACACCATCATGACCCTCACCAACCTCTCAGGTCCCTACTCCTACTGCAACACGACC
  TTGGACCAGATCGGAACGTGCTGGCCCCGCAGCGCTGCCGGAGCCCTCGTGGAGAGGCCGTGCCCCGAGT
  ACTTCAACGGCGTCAAGTACAACACGACCCGGAATGCCTATCGAGAATGCTTGGAGAATGGGACGTGGGC
  CTCAAAGATCAACTACTCACAGTGTGAGCCCATTTTGGATGACAAGCAGAGGAAGTATGACCTGCACTAC
  CGCATCGCCCTTGTCGTCAACTACCTGGGCCACTGCGTATCTGTGGCAGCCCTGGTGGCCGCCTTCCTGC
  TTTTCCTGGCCCTGCGGAGCATTCGCTGTCTGCGGAATGTGATTCACTGGAACCTCATCACCACCTTTAT
  CCTGCGAAATGTCATGTGGTTCCTGCTGCAGCTCGTTGACCATGAAGTGCACGAGAGCAATGAGGTCTGG
  TGCCGCTGCATCACCACCATCTTCAACTACTTCGTGGTGACCAACTTCTTCTGGATGTTTGTGGAAGGCT
  GCTACCTGCACACGGCCATTGTCATGACCTACTCCACTGAGCGCCTGCGCAAGTGCCTCTTCCTCTTCAT
  CGGATGGTGCATCCCCTTCCCCATCATCGTCGCCTGGGCCATCGGCAAGCTCTACTATGAGAATGAACAG
  TGCTGGTTTGGCAAGGAGCCTGGCGACCTGGTGGACTACATCTACCAAGGCCCCATCATTCTCGTGCTCC
  TGATCAATTTCGTATTTCTGTTCAACATCGTCAGGATCCTAATGACAAAGTTACGCGCGTCCACCACATC
  CGAGACAATCCAGTACAGGAAGGCAGTGAAGGCCACCCTGGTGCTCCTGCCCCTCCTGGGCATCACCTAC
  ATGCTCTTCTTCGTCAATCCCGGGGAGGACGACCTGTCACAGATCATGTTCATCTATTTCAACTCCTTCC
  TGCAGTCGTTCCAGGGTTTCTTCGTGTCTGTCTTCTACTGCTTCTTCAATGGAGAGGTGCGCTCAGCCGT
  GAGGAAGAGGTGGCACCGCTGGCAGGACCATCACTCCCTTCGAGTCCCCATGGCCCGGGCCATGTCCATC
  CCTACATCACCCACACGGATCAGCTTCCACAGCATCAAGCAGACGGCCGCTGTGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001202475
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001202475.1, NP_001189404.1
• RefSeq Size: 2904 bp
• RefSeq ORF: 1317 bp
• Locus ID: 1395
• Cytogenetics: 7p14.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'The protein encoded by this gene belongs to the G-protein coupled receptor 2 family, and the subfamily of corticotropin releasing hormone receptor. This receptor shows high affinity for corticotropin releasing hormone (CRH), and also binds CRH-related peptides such as urocortin. CRH is synthesized in the hypothalamus, and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge. Studies in mice suggest that this receptor maybe involved in mediating cardiovascular homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jan 2011]'
  Transcript Variant: This variant (2) contains additional in-frame coding exons at the 5' end compared to variant 1, resulting in an isoform (2, also known as beta isoform) with a longer and distinct N-terminus compared to isoform 1. This receptor subtype was shown to mediate urocortin-induced MAPK activation in human myometrium (PMID:11117536).