MBD1 (NM_001204137) Human Untagged Clone

CAT#: SC331496

MBD1 (untagged) - Homo sapiens methyl-CpG binding domain protein 1 (MBD1), transcript variant 7

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MBD1
• Synonyms: CXXC3; PCM1; RFT
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001204137, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGAGGACTGGCTGGACTGCCCGGCCCTGGGCCCTGGCTGGAAGCGCCGCGAAGTCTTTCGCAAGT
  CAGGGGCCACCTGTGGACGCTCAGACACCTATTACCAGAGCCCCACAGGAGACAGGATCCGAAGCAAAGT
  TGAGCTGACTCGATACCTGGGCCCTGCGTGTGATCTCACCCTCTTCGACTTCAAACAAGGCATCTTGTGC
  TATCCAGCCCCCAAGGCCCATCCCGTGGCGGTTGCCAGCAAGAAGCGAAAGAAGCCTTCAAGGCCAGCCA
  AGACTCGGAAACGTCAGGTTGGACCCCAGAGTGGTGAGGTCAGGAAGGAGGCCCCGAGGGATGAGACCAA
  GGCTGACACTGACACAGCCCCAGCTTCATTCCCTGCTCCTGGGTGCTGTGAGAACTGTGGAATCAGCTTC
  TCAGGGGATGGCACCCAAAGGCAGCGGCTCAAAACGTTGTGCAAAGACTGTCGAGCACAGAGAATTGCCT
  TCAACCGGGAACAGAGAATGTTTAAGCGTGTGGGCTGTGGGGAGTGTGCAGCCTGCCAGGTAACAGAAGA
  CTGTGGGGCCTGCTCCACCTGCCTCCTGCAGCTGCCCCATGATGTGGCATCGGGGCTGTTCTGCAAGTGT
  GAACGGAGACGCTGCCTCCGGATTGTGGAAAGGAGCCGAGGGTGTGGAGTATGCCGGGGCTGTCAGACCC
  AAGAGGATTGTGGCCATTGCCCCATCTGCCTTCGCCCTCCCCGCCCTGGTCTCAGGCGCCAGTGGAAATG
  TGTCCAGCGACGTTGCCTACGGCACCTTGCTCACCGCCTGCGTCGCCGTCATCAGAGATGTCAGCGACGC
  ACTCCCCTGGCTGTGGCTCCCCCAACTGGTAAACATGCCCGCCGCAAGGGAGGCTGTGACTCCAAGATGG
  CTGCCAGGCGGCGCCCCGGAGCCCAGCCACTGCCTCCACCACCCCCATCACAGTCCCCAGAGCCCACAGA
  GCCGCACCCCAGAGCCCTGGCCCCCTCGCCACCTGCCGAGTTCATCTATTACTGTGTAGACGAGGACGAG
  CTACAGCCCTACACGAACCGCCGGCAGAACCGCAAGTGCGGGGCCTGTGCAGCCTGCCTACGGCGGATGG
  ACTGTGGCCGCTGCGACTTCTGCTGCGACAAGCCCAAATTCGGGGGCAGCAACCAGAAGCGCCAGAAGTG
  TCGTTGGCGCCAATGCCTGCAGTTTGCCATGAAGCGGCTGCTGCCCAGTGTCTGGTCAGAGTCTGAGGAT
  GGGGCAGGATCGCCCCCACCTTACCGTCGTCGAAAGAGGCCCAGCTCTGCCCGACGGCACCATCTTGGCC
  CTACCTTGAAGCCCACCTTGGCTACACGCACAGCCCAACCAGACCATACCCAGGCTCCAACGAAGCAGGA
  AGCAGGTGGTGGCTTTGTGCTGCCCCCGCCTGGCACTGACCTTGTGTTTTTACGGGAAGGCGCAAGCAGT
  CCTGTGCAGGTGCCGGGCCCTGTTGCAGCTTCCACAGAAGCCCTGTTGCAGGAGGCCCAGTGCTCTGGCC
  TGAGTTGGGTTGTGGCCTTACCCCAGGTGAAGCAAGAGAAGGCGGATACCCAGGACGAGTGGACACCAGG
  CACAGCTGTCCTGACTTCTCCCGTATTGGTGCCTGGCTGCCCTAGCAAGGCAGTAGACCCAGGCCTGCCT
  TCTGTGAAGCAAGAGCCACCTGACCCAGAGGAGGACAAGGAGGAGAACAAGGATGATTCTGCCTCCAAAT
  TGGCCCCAGAGGAAGAGGCAGGAGGGGCTGGCACACCCGTGATCACGGAGATTTTCAGCCTGGGTGGAAC
  CCGCTTCCGAGATACAGCAGTCTGGTTGCCAAGGTCCAAAGACCTTAAAAAACCTGGAGCTAGAAAGCAG
  TAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001204137
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204137.1, NP_001191066.1
• RefSeq Size: 2796 bp
• RefSeq ORF: 1893 bp
• Locus ID: 4152
• Cytogenetics: 18q21.1
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (7) contains an additional in-frame coding exon, and has a different 3' UTR compared to variant 1. This results in a longer isoform (7, also known as RFT-A) with an extra protein segment not found in isoform 1. This isoform has been shown to repress FGF-2 expression at the transcriptional level, and may be involved in FGF-2-dependent tumor progression (PMID:10187827).