MBD1 (NM_001204140) Human Untagged Clone

CAT#: SC331499

MBD1 (untagged) - Homo sapiens methyl-CpG binding domain protein 1 (MBD1), transcript variant 9

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MBD1
• Synonyms: CXXC3; PCM1; RFT
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001204140, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGAGGACTGGCTGGACTGCCCGGCCCTGGGCCCTGGCTGGAAGCGCCGCGAAGTCTTTCGCAAGT
  CAGGGGCCACCTGTGGACGCTCAGACACCTATTACCAGAGCCCCACAGGAGACAGGATCCGAAGCAAAGT
  TGAGCTGACTCGATACCTGGGCCCTGCGTGTGATCTCACCCTCTTCGACTTCAAACAAGGCATCTTGTGC
  TATCCAGCCCCCAAGGCCCATCCCGTGGCGGTTGCCAGCAAGAAGCGAAAGAAGCCTTCAAGGCCAGCCA
  AGACTCGGAAACGTCAGGTTGGACCCCAGAGTGGTGAGGTCAGGAAGGAGGCCCCGAGGGATGAGACCAA
  GGCTGACACTGACACAGCCCCAGCTTCATTCCCTGCTCCTGGGTGCTGTGAGAACTGTGGAATCAGCTTC
  TCAGGGGATGGCACCCAAAGGCAGCGGCTCAAAACGTTGTGCAAAGACTGTCGAGCACAGAGAATTGCCT
  TCAACCGGGAACAGAGAATGTTTAAGCGTGTGGGCTGTGGGGAGTGTGCAGCCTGCCAGGTAACAGAAGA
  CTGTGGGGCCTGCTCCACCTGCCTCCTGCAGCTGCCCCATGATGTGGCATCGGGGCTGTTCTGCAAGTGT
  GAACGGAGACGCTGCCTCCGGATTGTGGAAAGGAGCCGAGGGTGTGGAGTATGCCGGGGCTGTCAGACCC
  AAGAGGATTGTGGCCATTGCCCCATCTGCCTTCGCCCTCCCCGCCCTGGTCTCAGGCGCCAGTGGAAATG
  TGTCCAGCGACGTTGCCTACGGCACCTTGCTCACCGCCTGCGTCGCCGTCATCAGAGATGTCAGCGACGC
  ACTCCCCTGGCTGTGGCTCCCCCAACTGGTAAACATGCCCGCCGCAAGGGAGGCTGTGACTCCAAGATGG
  CTGCCAGGCGGCGCCCCGGAGCCCAGCCACTGCCTCCACCACCCCCATCACAGTCCCCAGAGCCCACAGA
  GCCGCACCCCAGAGCCCTGGCCCCCTCGCCACCTGCCGAGTTCATCTATTACTGTGTAGACGAGGACGAG
  CTAAAGCGGCTGCTGCCCAGTGTCTGGTCAGAGTCTGAGGATGGGGCAGGATCGCCCCCACCTTACCGTC
  GTCGAAAGAGGCCCAGCTCTGCCCGACGGCACCATCTTGGCCCTACCTTGAAGCCCACCTTGGCTACACG
  CACAGCCCAACCAGACCATACCCAGGCTCCAACGAAGCAGGAAGCAGGTGGTGGCTTTGTGCTGCCCCCG
  CCTGGCACTGACCTTGTGTTTTTACGGGAAGGCGCAAGCAGTCCTGTGCAGGTGCCGGGCCCTGTTGCAG
  CTTCCACAGAAGCCCTGTTGCAGGAGGCCCAGTGCTCTGGCCTGAGTTGGGTTGTGGCCTTACCCCAGGT
  GAAGCAAGAGAAGGCGGATACCCAGGACGAGTGGACACCAGGCACAGCTGTCCTGACTTCTCCCGTATTG
  GTGCCTGGCTGCCCTAGCAAGGCAGTAGACCCAGGCCTGCCTTCTGTGAAGCAAGAGCCACCTGACCCAG
  AGGAGGACAAGGAGGAGAACAAGGATGATTCTGCCTCCAAATTGGCCCCAGAGGAAGAGGCAGGAGGGGC
  TGGCACACCCGTGATCACGGAGATTTTCAGCCTGGGTGGAACCCGCTTCCGAGATACAGCAGTCTGGTTG
  CCAAGGTCCAAAGACCTTAAAAAACCTGGAGCTAGAAAGCAGTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001204140
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204140.1, NP_001191069.1
• RefSeq Size: 2628 bp
• RefSeq ORF: 1725 bp
• Locus ID: 4152
• Cytogenetics: 18q21.1
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (9) is the same as variant 7, except lacks an in-frame coding exon, resulting in an isoform (9, also known as RFT-B) that is missing an internal protein segment compared to isoform 7. Unlike isoform 7, this isoform fails to bind FGF-2 promoter and repress FGF-2 gene expression (PMID:10187827).