LIM Kinase 1 (LIMK1) (NM_001204426) Human Untagged Clone

CAT#: SC331586

LIMK1 (untagged) - Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LIMK1
• Synonyms: LIMK; LIMK-1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001204426, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGTTGGCTTCAGCCCCAAGAAGACGCCGCTTCCTCCAGAGGGCTAAGTGTTGTGACTGCAGTGCCT
  CCCTGTCGCACCAGTACTATGAGAAGGATGGGCAGCTCTTCTGCAAGAAGGACTACTGGGCCCGCTATGG
  CGAGTCCTGCCATGGGTGCTCTGAGCAAATCACCAAGGGACTGGTTATGGTGGCTGGGGAGCTGAAGTAC
  CACCCCGAGTGTTTCATCTGCCTCACGTGTGGGACCTTTATCGGTGACGGGGACACCTACACGCTGGTGG
  AGCACTCCAAGCTGTACTGCGGGCACTGCTACTACCAGACTGTGGTGACCCCCGTCATCGAGCAGATCCT
  GCCTGACTCCCCTGGCTCCCACCTGCCCCACACCGTCACCCTGGTGTCCATCCCAGCCTCATCTCATGGC
  AAGCGTGGACTTTCAGTCTCCATTGACCCCCCGCACGGCCCACCGGGCTGTGGCACCGAGCACTCACACA
  CCGTCCGCGTCCAGGGAGTGGATCCGGGCTGCATGAGCCCAGATGTGAAGAATTCCATCCACGTCGGAGA
  CCGGATCTTGGAAATCAATGGCACGCCCATCCGAAATGTGCCCCTGGACGAGATTGACCTGCTGATTCAG
  GAAACCAGCCGCCTGCTCCAGCTGACCCTCGAGCATGACCCTCACGATACACTGGGCCACGGGCTGGGGC
  CTGAGACCAGCCCCCTGAGCTCTCCGGCTTATACTCCCAGCGGGGAGGCGGGCAGCTCTGCCCGGCAGAA
  ACCTGTCTTGAGGAGCTGCAGCATCGACAGGTCTCCGGGCGCTGGCTCACTGGGCTCCCCGGCCTCCCAG
  CGCAAGGACCTGGGTCGCTCTGAGTCCCTCCGCGTAGTCTGCCGGCCACACCGCATCTTCCGGCCGTCGG
  ACCTCATCCACGGGGAGGTGCTGGGCAAGGGCTGCTTCGGCCAGGCTATCAAGGTGACACACCGTGAGAC
  AGGTGAGGTGATGGTGATGAAGGAGCTGATCCGGTTCGACGAGGAGACCCAGAGGACGTTCCTCAAGGAG
  GTGAAGGTCATGCGATGCCTGGAACACCCCAACGTGCTCAAGTTCATCGGGGTGCTCTACAAGGACAAGA
  GGCTCAACTTCATCACTGAGTACATCAAGGGCGGCACGCTCCGGGGCATCATCAAGAGCATGGACAGCCA
  GTACCCATGGAGCCAGAGAGTGAGCTTTGCCAAGGACATCGCATCAGGGATGGCCTACCTCCACTCCATG
  AACATCATCCACCGAGACCTCAACTCCCACAACTGCCTGGTCCGCGAGAACAAGAATGTGGTGGTGGCTG
  ACTTCGGGCTGGCGCGTCTCATGGTGGACGAGAAGACTCAGCCTGAGGGCCTGCGGAGCCTCAAGAAGCC
  AGACCGCAAGAAGCGCTACACCGTGGTGGGCAACCCCTACTGGATGGCACCTGAGATGATCAACGGCCGC
  AGCTATGATGAGAAGGTGGATGTGTTCTCCTTTGGGATCGTCCTGTGCGAGATCATCGGGCGGGTGAACG
  CAGACCCTGACTACCTGCCCCGCACCATGGACTTTGGCCTCAACGTGCGAGGATTCCTGGACCGCTACTG
  CCCCCCAAACTGCCCCCCGAGCTTCTTCCCCATCACCGTGCGCTGTTGCGATCTGGACCCCGAGAAGAGG
  CCATCCTTTGTGAAGCTGGAACACTGGCTGGAGACCCTCCGCATGCACCTGGCCGGCCACCTGCCACTGG
  GCCCACAGCTGGAGCAGCTGGACAGAGGTTTCTGGGAGACCTACCGGCGCGGCGAGAGCGGACTGCCTGC
  CCACCCTGAGGTCCCCGACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001204426
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204426.1, NP_001191355.1
• RefSeq Size: 3176 bp
• RefSeq ORF: 1842 bp
• Locus ID: 3984
• Cytogenetics: 7q11.23
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• Gene Summary: 'There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (2) lacks multiple exons at the 5' end and uses a different start codon, compared to variant 1. This variant encodes an isoform (2) with a shorter and distinct N-terminus that lacks one of two LIM zinc-binding domains, compared to isoform 1.