BRF1 (NM_001242788) Human Untagged Clone

CAT#: SC331858

BRF1 (untagged) - Homo sapiens BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit (BRF1), transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BRF1
• Synonyms: BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p; TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001242788, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACGGGCCGCGTGTGCCGCGGTTGCGGCGGCACGGACATCGAGCTGGACGCGGCGCGCGGGGACGCGG
  TGTGCACCGCCTGCGGCTCAGTGCTGGAGGACAACATCATCGTGTCCGAGGTGCAGTTCGTGGAGAGCAG
  CGGCGGCGGCTCCTCGGCCGTGGGCCAGTTCGTGTCCCTGGACGGGAGGCGCCACATCCACCACCTGGGG
  AACCAGCTGCAGCTGAACCAGCACTGCCTGGACACCGCCTTCAACTTCTTCAAGATGGCCGTGAGCAGGC
  ACCTGACCCGCGGCCGGAAGATGGCCCACGTGATTGCTGCCTGCCTCTACCTGGTCTGCCGTACGGAGGG
  CACGCCGCACATGCTCCTGGACCTCAGCGACCTGCTCCAGGTGAATGTGTACGTGCTTGGAAAGACGTTT
  CTTCTCTTGGCAAGAGAGCTCTGCATCAATGCGCCGGCCATAGACCCGTGCCTGTATATTCCACGCTTTG
  CGCACCTGCTGGAATTCGGGGAGAAGAACCACGAGGTGTCCATGACTGCCCTGAGGCTCCTACAGAGGAT
  GAAGCGGGACTGGATGCACACAGGCCGGCGCCCCTCGGGCCTCTGCGGAGCAGCGCTCCTGGTTGCAGCC
  AGAATGCATGACTTCAGGAGGACTGTGAAGGAGGTCATCAGTGTGGTCAAAGTGTGTGAGTCCACGCTGC
  GGAAGAGGCTCACGGAATTTGAAGACACCCCCACCAGTCAGTTGACCATTGATGAGTTCATGAAGATCGA
  CCTGGAGGAGGAGTGCGACCCCCCCTCGTACACAGCTGGGCAGAGGAAGCTGCGGATGAAGCAGCTTGAA
  CAAGTCCTGTCAAAAAAACTGGAGGAGGTTGAAGGTGAAATATCCAGTTACCAGGATGCAATTGAGATTG
  AACTAGAAAACAGCCGGCCAAAGGCCAAGGGGGGCCTGGCCAGCCTGGCAAAAGATGGCTCCACCGAGGA
  CACCGCGTCCAGCTTGTGTGGCGAGGAGGACACAGAGGACGAGGAGCTGGAAGCCGCGGCCAGCCACCTG
  AACAAAGACTTATACCGGGAGCTCCTTGGTGGTGCCCCCGGCAGCTCGGAAGCAGCAGGAAGCCCCGAGT
  GGGGCGGCAGACCTCCGGCCCTGGGGTCCCTGCTGGACCCCCTCCCCACTGCAGCCAGCCTGGGCATCTC
  AGACTCCATCCGCGAATGCATCTCCTCTCAGAGCAGCGACCCCAAAGATGCTTCAGGAGACGGTGAGCTG
  GACCTCAGTGGCATTGATGACCTGGAGATTGACAGGTACATCCTGAATGAGTCGGAAGCCCGCGTGAAGG
  CCGAGCTGTGGATGAGGGAGAACGCCGAGTACCTGCGGGAACAGAGGGAAAAAGAAGCAAGAATAGCGAA
  AGAGAAGGAGCTCGGCATCTACAAGGAACACAAGCCCAAGAAGTCTTGCAAGCGACGGGAGCCAATTCAG
  GCCAGTACCGCCAGGGAGGCCATCGAGAAGATGCTGGAGCAGAAGAAGATCTCCAGCAAGATCAATTATA
  GCGTGCTCCGGGGCCTCAGCAGCGCCGGCGGGGGCAGTCCGCACAGGGAGGATGCACAGCCCGAGCATAG
  CGCCAGTGCCAGGAAGCTGTCACGAAGGAGGACGCCGGCCAGCAGAAGTGGGGCTGACCCTGTGACCAGT
  GTGGGGAAAAGGTTGAGGCCTCTGGTGTCTACGCAGCCAGCAAAGAAGGTGGCCACGGGAGAGGCTTTGC
  TCCCAAGCTCTCCCACCCTCGGAGCTGAGCCTGCCAGGCCCCAGGCGGTGCTGGTGGAGAGCGGGCCCGT
  GTCATACCACGCCGACGAGGAGGCTGACGAGGAGGAGCCTGACGAGGAGGACGGGGAGCCCTGCGTCAGT
  GCCCTGCAGATGATGGGCAGCAACGACTATGGCTGTGATGGCGATGAGGACGACGGCTACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001242788
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242788.1, NP_001229717.1
• RefSeq Size: 3596 bp
• RefSeq ORF: 1953 bp
• Locus ID: 2972
• Cytogenetics: 14q32.33
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011]'
  Transcript Variant: This variant (6) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (6) has the same N- and C-termini but is shorter compared to isoform 1.