PDE1B (NM_001288768) Human Untagged Clone

CAT#: SC335865

PDE1B (untagged) - Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE1B
• Synonyms: HEL-S-79p; PDE1B1; PDES1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001288768, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCCGGAGAACATACACCTCTGTGGGCCCCACTTACTCTACTGCGGTTCTCAACTGTCTCAAGAACC
  TGGATCTCTGGTGCTTTGATGTCTTTTCCTTGAACCAGGCAGCAGATGACCATGCCCTGAGGACCATTGT
  TTTTGAGTTGCTGACTCGGCATAACCTCATCAGCCGCTTCAAGATTCCCACTGTGTTTTTGATGAGTTTC
  CTGGATGCCTTGGAGACAGGCTATGGGAAGTACAAGAATCCTTACCACAACCAGATCCACGCAGCCGATG
  TTACCCAGACAGTCCATTGCTTCTTGCTCCGCACAGGGATGGTGCACTGCCTGTCGGAGATTGAGCTCCT
  GGCCATCATCTTTGCTGCAGCTATCCATGATTATGAGCACACGGGCACTACCAACAGCTTCCACATCCAG
  ACCAAGTCAGAATGTGCCATCGTGTACAATGATCGTTCAGTGCTGGAGAATCACCACATCAGCTCTGTTT
  TCCGATTGATGCAGGATGATGAGATGAACATTTTCATCAACCTCACCAAGGATGAGTTTGTAGAACTCCG
  AGCCCTGGTCATTGAGATGGTGTTGGCCACAGACATGTCCTGCCATTTCCAGCAAGTGAAGACCATGAAG
  ACAGCCTTGCAACAGCTGGAGAGGATTGACAAGCCCAAGGCCCTGTCTCTACTGCTCCATGCTGCTGACA
  TCAGCCACCCAACCAAGCAGTGGTTGGTCCACAGCCGTTGGACCAAGGCCCTCATGGAGGAATTCTTCCG
  TCAGGGTGACAAGGAGGCAGAGTTGGGCCTGCCCTTTTCTCCACTCTGTGACCGCACTTCCACTCTAGTG
  GCACAGTCTCAGATAGGGTTCATCGACTTCATTGTGGAGCCCACATTCTCTGTGCTGACTGACGTGGCAG
  AGAAGAGTGTTCAGCCCCTGGCGGATGAGGACTCCAAGTCTAAAAACCAGCCCAGCTTTCAGTGGCGCCA
  GCCCTCTCTGGATGTGGAAGTGGGAGACCCCAACCCTGATGTGGTCAGCTTTCGTTCCACCTGGGTCAAG
  CGCATTCAGGAGAATAAGCAGAAATGGAAGGAACGGGCAGCAAGTGGCATCACCAACCAGATGTCCATTG
  ACGAGCTGTCCCCCTGTGAAGAAGAGGCCCCCCCATCCCCTGCCGAAGATGAACACAACCAGAATGGGAA
  TCTGGATTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001288768
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288768.1, NP_001275697.1
• RefSeq Size: 3525 bp
• RefSeq ORF: 1200 bp
• Locus ID: 5153
• Cytogenetics: 12q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (3) differs in its 5' UTR and uses a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Variants 3 and 5 both encode the same isoform (3).