5HT4 Receptor (HTR4) (NM_001286410) Human Untagged Clone

CAT#: SC335945

HTR4 (untagged) - Human 5-hydroxytryptamine (serotonin) receptor 4, G protein-coupled (HTR4), transcript variant c

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HTR4
• Synonyms: 5-HT4; 5-HT4R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001286410, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACAAACTTGATGCTAATGTGAGTTCTGAGGAGGGTTTCGGGTCAGTGGAGAAGGTGGTGCTGCTCA
  CGTTTCTCTCGACGGTTATCCTGATGGCCATCTTGGGGAACCTGCTGGTGATGGTGGCTGTGTGCTGGGA
  CAGGCAGCTCAGGAAAATAAAAACAAATTATTTCATTGTATCTCTTGCTTTTGCGGATCTGCTGGTTTCG
  GTGCTGGTGATGCCCTTTGGTGCCATTGAGCTGGTTCAAGACATCTGGATTTATGGGGAGGTGTTTTGTC
  TTGTTCGGACATCTCTGGACGTCCTGCTCACAACGGCATCGATTTTTCACCTGTGCTGCATTTCTCTGGA
  TAGGTATTACGCCATCTGCTGCCAGCCTTTGGTCTATAGGAACAAGATGACCCCTCTGCGCATCGCATTA
  ATGCTGGGAGGCTGCTGGGTCATCCCCACGTTTATTTCTTTTCTCCCTATAATGCAAGGCTGGAATAACA
  TTGGCATAATTGATTTGATAGAAAAGAGGAAGTTCAACCAGAACTCTAACTCTACGTACTGTGTCTTCAT
  GGTCAACAAGCCCTACGCCATCACCTGCTCTGTGGTGGCCTTCTACATCCCATTTCTCCTCATGGTGCTG
  GCCTATTACCGCATCTATGTCACAGCTAAGGAGCATGCCCATCAGATCCAGATGTTACAACGGGCAGGAG
  CCTCCTCCGAGAGCAGGCCTCAGTCGGCAGACCAGCATAGCACTCATCGCATGAGGACAGAGACCAAAGC
  AGCCAAGACCCTGTGCATCATCATGGGTTGCTTCTGCCTCTGCTGGGCACCATTCTTTGTCACCAATATT
  GTGGATCCTTTCATAGACTACACTGTCCCTGGGCAGGTGTGGACTGCTTTCCTCTGGCTCGGCTATATCA
  ATTCCGGGTTGAACCCTTTTCTCTACGCCTTCTTGAATAAGTCTTTTAGACGTGCCTTCCTCATCATCCT
  CTGCTGTGATGATGAGCGCTACCGAAGACCTTCCATTCTGGGCCAGACTGTCCCTTGTTCAACCACAACC
  ATTAATGGATCCACACATGTACTAAGTTCTGGAACTGAAACCGACAGGAAGAAACTTTGGAATAAGGAAG
  AGAAGATTGACCAAACCATCCAAATGCCAAAAAGAAAAAGAAAAAAAAAAGCCTCTCTGTCCTATGAAGA
  CTTAATTCTGTTGGGGAGAAAGTCATGTTTTAGGGAAGGAAAATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286410
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286410.1, NP_001273339.1
• RefSeq Size: 1283 bp
• RefSeq ORF: 1236 bp
• Locus ID: 3360
• Cytogenetics: 5q32
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010]'
  Transcript Variant: This variant (c) has a shorter 5' UTR and uses an alternate exon in the 3' UTR and coding region. The encoded isoform (c) is shorter and has a distinct C-terminus compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.